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Shop: MushCult - Live Mushroom Cultures Black morel (Morchella importuna) live culture on agar   Amazon ½ Pint Jars, Vermiculite

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OfflineOpin
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Registered: 03/12/02
Posts: 8
Loc: texass
Last seen: 16 years, 7 months
Sterilization and Growth
    #581725 - 03/17/02 10:58 PM (16 years, 9 months ago)

I have a vegetable steamer that i used to sterilize my jars... i set up a test with three jars to see which ones wouldn't get contaminated and it seems that none of them have so far but the one that only stayed for 30 mins had a small black dot i thought was contamination but it hasn't grown any and it has a bunch of white growth around it so i think the spot was just a piece of the vermiculite.... That jar has the most growth and the jars that stayed for 45 and 60 mins seem to have not much growth at all.... so my question is , is 30 mins a sufficient amount of time to sterlize a jar in a vegetable steamer, am i over drying at 45 and 60 mins, and how soon should i expect noticeable growth? It's been 4 full days now... Thanx for any help it's much appreciated!


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OfflineMushyMay
Brian Eno is mypersonal God FNORD

Registered: 02/19/02
Posts: 423
Loc: ACT, Australia
Last seen: 1 year, 1 month
Re: Sterilization and Growth [Re: Opin]
    #581767 - 03/17/02 11:49 PM (16 years, 9 months ago)

>> is 30 mins a sufficient amount of time to sterlize a jar in a vegetable steamer

I'd say no, a PC reaches 250?C and you need a full hour of that to sterilise a jar. A steamer would be unlikely to reach more than 100?C (without adding anything to the water.)

>>am i over drying at 45 and 60 mins

If you have lids on the jars (loosely) and foil over the top, they should not dry out.


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MushyMay is a fictional character, as a result any information provided by MushyMay is also fictitious.


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OfflineOpin
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Registered: 03/12/02
Posts: 8
Loc: texass
Last seen: 16 years, 7 months
Re: Sterilization and Growth [Re: MushyMay]
    #581804 - 03/18/02 12:30 AM (16 years, 9 months ago)

so maybe i should try an hour and half? 2 hrs? and if i dont end up getting any contamination should i just leave it at 30 mins?and how long do i wait before inoculating the jars? thanx for your reply


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Shop: MushCult - Live Mushroom Cultures Black morel (Morchella importuna) live culture on agar   Amazon ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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