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rackemwillie
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Registered: 06/29/06
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Last seen: 16 years, 9 months
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What should I do?(pics added)
#5814992 - 07/03/06 06:58 AM (17 years, 6 months ago) |
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My neighbor and only mycology buddy left town for college and gave me a tub of colonizing GT. Only problem is it wont colonize. This was his first tub grow and from what he told me he made a few mistakes.
Its been incubating in his garage anywhere from 85 at night and prolly much higher temps in the morning/afternoon in this hot florida weather. He did not use enough spawn He packed down the hpoo/straw a little too hard IMO. (he told me he pressed down on it with his hands using his body weight )
for the past 4 days the myc has grown a little on the fuzzy side but it hasnt spread. theres about 15-20 spots on the top I can see where little puff balls of myc are....but nothing has changed in 7 days!
should i mix the hpoo/straw up a little bit since its so tightly packed? give it a few more days?
any advice would be great cause im really stumped on this one. i already put the tub in my closet. temps in the room are in the mid 70's. also there is only 2 holes on the smaller side with polyfill.
Edited by rackemwillie (07/03/06 07:37 AM)
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UnderNose
all out of bubble gum


Registered: 03/04/06
Posts: 1,612
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what dose it smell like Are you sure its mycelium it could be Cobweb mold do you have pics can you explain it better
Look here for some pics see if its the same stuff http://www.shroomery.org/index.php/par/23418
-------------------- LAGM 2.022  
Edited by UnderNose (07/03/06 07:56 AM)
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rackemwillie
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Re: What should I do? [Re: UnderNose]
#5815021 - 07/03/06 07:18 AM (17 years, 6 months ago) |
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def not cobweb. first time i ever saw cobweb mold i knew it instantly. the spawn was BRF..the areas where the myc are is the actual spawn so I have seen no growth. the areas where myc are look healthy to me. i will get a picture up asap. no batteries so im gona rip some outa someone TV controller :-p
Edited by rackemwillie (07/03/06 08:12 AM)
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rackemwillie
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Re: What should I do? [Re: UnderNose]
#5815036 - 07/03/06 07:36 AM (17 years, 6 months ago) |
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oh yeah and the smell. since i got the hpoo for him it smells just like the stuff i had when it was colonizing.



Edited by rackemwillie (07/03/06 07:40 AM)
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UnderNose
all out of bubble gum


Registered: 03/04/06
Posts: 1,612
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yea that looks pretty tight. the myc would be having a hard time.. how moist is the substrate?
Loosening it up with your hands could introduce contams. especially to a uncolonized substrate. Maybe some kind of tool that has been sterilized. Like a fork but bigger. Do some grid work, stab lift, stab lift , to aerate the substrate a little. don't break it up to much But be careful make sure the air around you is clean, still and all that.
Edited by UnderNose (07/03/06 07:44 AM)
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rackemwillie
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Re: What should I do? [Re: UnderNose]
#5815051 - 07/03/06 07:44 AM (17 years, 6 months ago) |
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well my buddy opened it once since he had it. ive checked on it about 3 times. this is mikes hpoo/straw pre pasteurized stuff. I would say its about the same moisture as when i took my stuff out of the bag.... if anything a little less.
my concerns were about the low spawn ratio but wouldnt that mean prone to contam. no contams yet.
also how tight the hpoo is. should i mix it up a little? if so how? should i transplant to another container?
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UnderNose
all out of bubble gum


Registered: 03/04/06
Posts: 1,612
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low spawn means slow, slow means contams have more of a chance but thats not bad just takes a little longer
You could cut it in half put half in another box and break them up but it's risky
I would wait a little more and see what happens.
Have you heard of using coffee grounds, speeds up colonization.
Edited by UnderNose (07/03/06 08:17 AM)
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rackemwillie
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Last seen: 16 years, 9 months
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Re: What should I do? [Re: UnderNose]
#5815063 - 07/03/06 08:00 AM (17 years, 6 months ago) |
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well ive heard about coffee grounds being used in mycology but i dont know anything about it. if i dont hear from anyone who really knows what to do for sure... i'll just wait a couple more days and if i dont see any progress im gona operate.
Edited by rackemwillie (07/03/06 08:11 AM)
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UnderNose
all out of bubble gum


Registered: 03/04/06
Posts: 1,612
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Re: What should I do? [Re: UnderNose]
#5815073 - 07/03/06 08:12 AM (17 years, 6 months ago) |
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Quote:
UnderNose said: Maybe some kind of tool that has been sterilized. Like a fork but bigger. Do some grid work, stab lift, stab lift , to aerate the substrate a little. don't break it up to much But be careful make sure the air around you is clean, still and all that.
If you can be 95 percent sterile you could break it up with your hands loosen and mix it up. I do this to my spawn bags to speed up colonization but they are in sealed bags this is risky with a mono tub in open air
-------------------- LAGM 2.022  
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EquilibriuM
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Registered: 07/17/05
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I would give it time. Looks alright. be patient...
-------------------- HELP!!!!!!!!!
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rackemwillie
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Re: What should I do? [Re: UnderNose]
#5815076 - 07/03/06 08:18 AM (17 years, 6 months ago) |
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im pretty clean when doing anything b/c everything i do is open air. but ive never tried doing anything like this 
im pretty sure the myc is just having a tough time cause everything is so tight in there. i just wana be able to salvage this. or some of it. im hoping ill see some growth soon. ill either mix it up or cut it into sections. im gona ask a couple people would they would recommend doing even though im pretty sure ill get the same answers.
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rackemwillie
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Quote:
EquilibriuM said: I would give it time. Looks alright. be patient...
yeah ill check on it in 24-48 hours. its just been like that for so long.
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RogerRabbit
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One should never pack down a horse manure substrate. The only thing we pack down is straw. Manure should be left loose and fluffy. I doubt it will be able to colonize like that, and there's a good chance it will go south due to contamination in the center where no air can reach.
I'd suggest getting a room as clean as you can, then spray with oust or ten percent bleach to further clean the air. Use latex gloves or a clean spoon and stir the entire contents to mix and fluff it up. It wouldn't hurt at all to have a bit of moistened vermiculite to mix in as well, but the important thing is to get the substrate loose and aerated so the mycelium can penetrate. Good luck. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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EquilibriuM
dream stalker

Registered: 07/17/05
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Quote:
RogerRabbit said: One should never pack down a horse manure substrate. The only thing we pack down is straw. Manure should be left loose and fluffy. I doubt it will be able to colonize like that, and there's a good chance it will go south due to contamination in the center where no air can reach.
I'd suggest getting a room as clean as you can, then spray with oust or ten percent bleach to further clean the air. Use latex gloves or a clean spoon and stir the entire contents to mix and fluff it up. It wouldn't hurt at all to have a bit of moistened vermiculite to mix in as well, but the important thing is to get the substrate loose and aerated so the mycelium can penetrate. Good luck. RR
I like the moist vermiculite idea.
Also, in TMC he talks about commercial farmers using a press to pack down their substrate... any comment?
-------------------- HELP!!!!!!!!!
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rackemwillie
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Last seen: 16 years, 9 months
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im gona mix it around now. hope everything goes well.
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SpookerShroom
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Registered: 06/12/06
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Also you mentioned that you wern't sure of the max. temp the tub was subjected to.....I know that my garage gets up to 110F somtimes and im in a much cooler place then FL....if the myc was subjected to over 105-110 for more than a few hours it could all be dead...esp. with a bulk like that where it is self-heating as it is.
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rackemwillie
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Last seen: 16 years, 9 months
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yeah i was thinking about this. its a lil after noon and i put a temp meeter in the garage. im gona check on that now.
would it be possible to put these into seperate containers. that way maybe some will survive and some wont? instead of risking all my eggs in one basket kinda thing.
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rackemwillie
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garage wasnt over 90.
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Psychoslut
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Registered: 12/10/02
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Quote:
EquilibriuM said:
Quote:
RogerRabbit said: One should never pack down a horse manure substrate. The only thing we pack down is straw. Manure should be left loose and fluffy. I doubt it will be able to colonize like that, and there's a good chance it will go south due to contamination in the center where no air can reach.
I'd suggest getting a room as clean as you can, then spray with oust or ten percent bleach to further clean the air. Use latex gloves or a clean spoon and stir the entire contents to mix and fluff it up. It wouldn't hurt at all to have a bit of moistened vermiculite to mix in as well, but the important thing is to get the substrate loose and aerated so the mycelium can penetrate. Good luck. RR
I like the moist vermiculite idea.
Also, in TMC he talks about commercial farmers using a press to pack down their substrate... any comment?
Thats for button mushrooms. They use compost that has allot of straw in it so the mycelium can still proliferate through it. The denser the colonized material the more mushrooms it grows but it first has to colonize. If you add 25-50% moist chopped straw to your horse manure you can get away with compacting it.
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