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Invisiblellamabox
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: the man]
    #5824474 - 07/05/06 05:27 PM (17 years, 6 months ago)

I think Una had a really great thread on this subject...
Ccatus tissue culture


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OfflineSchwip
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Asante]
    #5824502 - 07/05/06 05:35 PM (17 years, 6 months ago)

Although i didnt read the entire post yet, this technique has proven to work very very well for both salvia and virola.

I think i saw some indepth 'teks' floating around on shaman austrilis or maybe the nook. But they are out there somewhere :smile:


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Invisiblellamabox
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Schwip]
    #5824538 - 07/05/06 05:41 PM (17 years, 6 months ago)

The one I just posted the link to is by Una from the nook.


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Invisiblemycogirl
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: llamabox]
    #5824715 - 07/05/06 06:20 PM (17 years, 6 months ago)

I got callus tissue first, and the hormone concentration I listed was for callus tissue actually, you need to decrease the NAA to a tenth of that to get shoots.

If you would like any further specific info, Please PM me.

Although, like I said, unless you absolutely can't propagate any other way, this is really not ideal.


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OfflinePsiloman
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Asante]
    #5846599 - 07/11/06 11:43 AM (17 years, 6 months ago)

My thread on Maxicrop :

http://www.shroomery.org/forums/showflat...rue#Post4167592

A thread i made on phytohormones mentioned here and alternative uses :

http://www.shroomery.org/forums/showflat...rue#Post4198829

Two ideas on tissue cultures :

1) One could use a tissue culture to save seedlings that are on the verge of dying.Did you ever have precious seedlings of rare species dying on you,with no way to save them? Cutting them up and transfering them on petri dishes to callous could give an alternative method of cultivating seedlings that are going to die.

2)How about tissue cultivation straight from seeds? One could take seed material and grow it on a petri dish,if the seeds prove too diffivult to germinate.The only prerequisite would be the seed to be "handleable" ,that is in a size that one could open and remove tissue.

Something else interesting with seeds : Do you know that the ploidy level in them is not uniform? All tissue is not 2n (that is 2 times the chromosomes half from pollen half from ovule) ,there is also 3n (triploid) tissue containing 2 times the maternal chromosomes plus one time the pollen chromosome (its because og how pollen fertilises the ovule).This could be also cultivated couldnt it? It would give sterile offsprings but there are tricks to turn them into fertise hexaploids.Even if thats not the target ,still a triploid could be interesting.

Have a look here : http://www.seedbiology.de/structure.asp#structure1


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Invisiblekarode13Facebook
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Psiloman]
    #5847432 - 07/11/06 03:28 PM (17 years, 6 months ago)

Here is photos of a new cultivar of Libertia sp.,grown by tissue culture in New Zealand.


Just posted these to give people an example,these plants are in the stage of being multiplied and the agar contains no root forming hormones.
I have no experience in micropropagation,but do occasionally deflask new plants.


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OfflineBaby_Hitler
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: mycogirl]
    #5848293 - 07/11/06 07:21 PM (17 years, 6 months ago)

Can you use peroxide in your media?


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OfflineFreeSporePrints
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Baby_Hitler]
    #8391511 - 05/12/08 02:20 AM (15 years, 8 months ago)

Hi mates,
i hope you can be interested to give a hand with our micropropagation project:

http://www.shroomery.org/forums/showflat.php?Cat=0&Number=8391485&page=0&vc=1&PHPSESSID=#Post8391485

Bye,
Fabio


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Offlineroyer
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Asante]
    #8395639 - 05/13/08 07:25 AM (15 years, 8 months ago)


please do not dig up old threads to plug a thread you have already started

thank you


Edited by royer9864 (05/13/08 07:30 AM)


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OfflineLuNaTiX
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Baby_Hitler]
    #8397038 - 05/13/08 02:37 PM (15 years, 8 months ago)

I was wondering if that would work too, I think its converted to water with time, I figure adding a little bit wouldnt hurt, maybe even decrease contamination rate.


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Offlinesalviabythe1000s
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Asante]
    #9953639 - 03/11/09 06:47 PM (14 years, 10 months ago)

its been done... see pic.



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Offlineethnoguy
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: salviabythe1000s]
    #9955121 - 03/11/09 09:52 PM (14 years, 10 months ago)

Oh no here comes a vendor plugging his store :rolleyes:. Whats going on around here?

EG


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Offlinesalviabythe1000s
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: ethnoguy]
    #9956185 - 03/12/09 01:06 AM (14 years, 10 months ago)

do you see me plugging? thou presumptuous one ...

by the way 1.0 mg 6-bap in 1/4 msg medium will give you L. williamsii buds like crazy!

for your growspace: try installing something called SHCS or sub terranean heating and cooling system. a great do it yourself low-cost hightechnology for keeping it cool in summer and warm in winter.

explained here: http://www.sunnyjohn.com/indexpages/shcs.htm


Edited by salviabythe1000s (03/12/09 01:21 AM)


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OfflineLegoulash
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: ethnoguy]
    #9956416 - 03/12/09 01:58 AM (14 years, 10 months ago)

Quote:

ethnoguy said:
Oh no here comes a vendor plugging his store :rolleyes:. Whats going on around here?

EG





What are you talking about, he posted a great picture that is directly related to the OPs topic. 

i have seen these liquid cultures a few times, mushmush.nl had a similar setup.

Do you grow any other plants with the same methods salvia?
What is your success rate with micro propagation ?


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Offlineethnoguy
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: Legoulash]
    #9956464 - 03/12/09 02:11 AM (14 years, 10 months ago)

Perhaps a little presumptuous. If thats the case sorry. The username, photo, and post count got me thinking. Just seen it at the growery.

EG


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Offlinesalviabythe1000s
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: ethnoguy]
    #9993656 - 03/18/09 07:16 AM (14 years, 10 months ago)

everything's cool EG!! did you check the shcs system?

yeah huge succes rate with salvia. it does very well in vitro, i do actually sell them so EG wasn't all wrong but its a modest buisness, (live salvia plants sell like shit) i do also multiply psychotria viridis and banisteriopsys caapi the 2 main ayahuasca trees. I used to sell to smart shops in the Netherlands a series of holy plants. No succes there though, people want instant gratification/satisfaction so prefer pills etc. I earn my living multiplying banana, ornamentals, and coldresistant tropical fruittrees (such as grow in the andes mountains) i started out micropropagating ethnobotanicals though. making extracts of mimosa hostilis is my hobby and god knows i love the dmt rush. So advice on micropropagation i would be willing to give, people interested in spreading salvia plants or whatever drop me a line.

greetings


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Offlineethnoguy
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: salviabythe1000s]
    #9993879 - 03/18/09 09:14 AM (14 years, 10 months ago)

lol. Damn, am I mod material or what?

EG


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Offlinetripp23
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: ethnoguy]
    #9997439 - 03/18/09 08:08 PM (14 years, 10 months ago)

woah.. wait... does this mean i can take a dried leaf and make it grow into an actual plant or does the leaf have to be still fresh???


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OfflineDr. uarewotueat
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Re: 1.000 cuttings from ONE LEAF ?! The Micropropagation project [Re: tripp23]
    #10019990 - 03/22/09 01:58 PM (14 years, 10 months ago)

the leaves need to be alive LOL...

those salvia tissue cultures are pretty common in europe, all my plants have been grown from one like that.



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