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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Seeing green (pics included)
    #5811049 - 07/01/06 10:22 PM (17 years, 6 months ago)

The pictures below are of 4 pf-tek jars, about 5 days into incubation. They were inoculated with liquid culture, b+ specifically. Obviously, they are contaminated, and normally I wouldn't even bother posting these pictures even in the contamination forum. But, what's bothering me is the fashion in which they were contaminated. Each of the four jars displayed the exact same pattern of growth. Long stripes, similar to how mycelium liquid cultures colonize a jar. I thoroughly sterilized the syringe I used to extract and inject the liquid culture. Leading me to believe that airborne spores etc. contaminating my syringe were not the problem.

The only two liquid cultures that were successful out of a batch of six were the two b+ jars, one honey and one Karo. The growth was snow white and wispy, and very explosive. Although it barely lasted 24 hours. Was what I injected indeed mycelium? I don't see how it could have been anything else.

As you can see, my luck with liquid cultures is very hit and miss, and inoculating jars directly with spores has not provided me with any results. I can't narrow down my problem, and for a n00b who just wants to see some bloody white mold colonize a jar or two this is very frustrating. This was my third attempt at the pf-tek. The first two attempts didn't grow. This one contamed. Any ideas, suggestions?







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"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


Edited by kranked (07/01/06 10:27 PM)


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OfflineSnaggletooth
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Registered: 10/24/05
Posts: 6,109
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Re: Seeing green (pics included) [Re: kranked]
    #5811058 - 07/01/06 10:27 PM (17 years, 6 months ago)

That is a very good example of LC contam,

I use bags, so when it dose happen I dont see that.


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Atheist Chat


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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: Seeing green (pics included) [Re: kranked]
    #5811074 - 07/01/06 10:31 PM (17 years, 6 months ago)

I bet ur liquid culture was contamed.
U get any successful jars from it?


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InvisibleAtheist
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Registered: 01/24/06
Posts: 13,705
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Re: Seeing green (pics included) [Re: SubGen1us]
    #5811156 - 07/01/06 11:05 PM (17 years, 6 months ago)

throw that shit out


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Offlinemikeownow
Humungus fungus

Registered: 09/01/05
Posts: 2,856
Loc: WA,USA
Last seen: 17 years, 3 months
Re: Seeing green (pics included) [Re: Atheist]
    #5811189 - 07/01/06 11:25 PM (17 years, 6 months ago)

wow u got the mean green real real bad.


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Re: Seeing green (pics included) [Re: SubGen1us]
    #5811245 - 07/01/06 11:52 PM (17 years, 6 months ago)

No they all turned out the same. Out of my six LC jars, only two grew, the b+. I made another two more b+ LC's on two separate occasions and they also failed to grow. I can't figure it out and am getting to the end of my rope here.


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Re: Seeing green (pics included) [Re: kranked]
    #5811253 - 07/01/06 11:55 PM (17 years, 6 months ago)

I'm going to sterilize the living shit out of a syringe and inoculate a fresh jar with the same LC to see if I can narrow down the source of the contam.

I still don't get it though, the growth in the LC looked exactly like mycelium. Snow white, wispy, it's fucking weird.


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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OfflineSnaggletooth
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Re: Seeing green (pics included) [Re: kranked]
    #5811287 - 07/02/06 12:07 AM (17 years, 6 months ago)

so dose the mycelium of the mean green, I think


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Atheist Chat


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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Re: Seeing green (pics included) [Re: Snaggletooth]
    #5811299 - 07/02/06 12:15 AM (17 years, 6 months ago)

Quote:

Snaggletooth said:
so dose the mycelium of the mean green, I think




Well, that is just fucking spectacular. I'm going crazy over why my LC's are failing.


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"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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OfflineTheFish
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Registered: 10/07/03
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Re: Seeing green (pics included) [Re: kranked]
    #5811304 - 07/02/06 12:17 AM (17 years, 6 months ago)

Try malt, dextrose, and a pressure cooker.


--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."

Jerry Garcia


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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Re: Seeing green (pics included) [Re: TheFish]
    #5811310 - 07/02/06 12:21 AM (17 years, 6 months ago)

Quote:

TheFish said:
Try malt, dextrose, and a pressure cooker.




Thanks for the idea, I'll look into that. I am curious though, is the malt/dextrose mixture more nutrient dense? Different kind of nutrients?


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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OfflineJSshroom
dont be paranoid, just aware

Registered: 06/16/05
Posts: 825
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Re: Seeing green (pics included) [Re: kranked]
    #5811377 - 07/02/06 12:56 AM (17 years, 6 months ago)

cant say but it is said to be the best LC. mabey for clarity. Yes I have had the LC woes myself. A thick ball of mycelium that is bacterially contamed. Mean green is white at first then the spore discharge is green. You need to start over from spores. then make an lc and run 1 jar. then try it. youll be able to tell if you syringe is contamed. unless you have agar which would show you faster.


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First Grow No more carpet tek


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OfflineYefet2000
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Re: Seeing green (pics included) [Re: JSshroom]
    #5812785 - 07/02/06 03:42 PM (17 years, 6 months ago)

How did you make the LC ?
What ingredients ?
did you PC it?
I use Honey water and it worked almost every time.
do you use Polyfill/Tyvek ?
Does your spore syringe gives the same result ? it might be the cause of all the contam ?
BTW: you said "Although it barely lasted 24 hours" what lasted the growth in the LC or in the BRF jar ?
anyway good luck.


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Me fail english ? Thats unpossible...


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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Re: Seeing green (pics included) [Re: Yefet2000]
    #5813874 - 07/02/06 10:14 PM (17 years, 6 months ago)

Quote:

Yefet2000 said:
How did you make the LC ?
What ingredients ?
did you PC it?
I use Honey water and it worked almost every time.
do you use Polyfill/Tyvek ?
Does your spore syringe gives the same result ? it might be the cause of all the contam ?
BTW: you said "Although it barely lasted 24 hours" what lasted the growth in the LC or in the BRF jar ?
anyway good luck.




Pic of my LC a week after I used the myc in a pf-jar...



1 tbs honey (clear honey)
Steam sterilized in pot with lid.
No polyfil or tyvek, just a band-aid over the hole.
I'm testing the same syringe tonight, I hope thats not the problem, it could mean bad news for a shroomery sponsor.
The growth in the LC barely lasted 24 hours, and as shown above was mean green after all.


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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OfflineOatman2000
-=Outa Space=-
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Re: Seeing green (pics included) [Re: kranked]
    #5815214 - 07/03/06 09:29 AM (17 years, 6 months ago)

maybee it's your spore?


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Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Re: Seeing green (pics included) [Re: Oatman2000]
    #5820343 - 07/04/06 03:08 PM (17 years, 6 months ago)

Maybe, my new LC seems to be doing ok. I'll know in another day or two if the problem was the spores.


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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