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lightningman123
psilocybe boy


Registered: 06/28/06
Posts: 74
Loc: michigan
Last seen: 16 years, 7 months
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FROM MYCOTOPIA!!!! cloning using dried mushrooms....yes its possible
#5808187 - 06/30/06 11:59 PM (17 years, 7 months ago) |
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I've tried this with dried agar. 20 days ago, I cut a couple of wedges from a colonized cubensis plate and put them in an empty plastic petri dish, which was stored at room temperature. After laying there and drying for 19 days, I placed one of them on a fresh non-peroxidated PDYA plate. The wedge looked really dry to me and was very hard. To my surprise, as I did not expect this result, there is growth today, only 1.5 days after I put it there:

Prepare the agar a bit on the wet side to help hydrate the tissue. Also, be ready with fresh plates to transfer into, because all the contamination on the dried fruit will also begin to grow. You'll have to transfer healthy mycelia as soon as you see it to a new petri dish. It generally takes ten days to two weeks, sometimes longer, for the dried fruit to begin to grow again. Unfortunately the contamination doesn't need that long, so you have a bit of work to do to clean up the culture, but it will work. It's been done for many years in the commercial mushroom industry. It's the standard way they keep and trade isolated strains of edible mushrooms. They desiccant dry the fruitbodies, then powder them. They store the powder until ready for use, then sprinkle it on agar to regenerate the strain. It stops senescence in its tracks.
Try it without soaking in peroxide first. That only weakens the mushroom myc so it doesn't take off as fast. Watch for the first signs of myc growth, and immediately transfer that to a new petri dish. Do this a time or two and you'll have a clean specimen, even from years old dried fruits

Find a good fruiting strain, then just keep some desiccant dried fruitbodies in a jar. When you want more, just clone a piece of the dry fruit into agar, and you have an instant clone. It will never degenerate further. This should last for many, many years.
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Morelman
LC Master


Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: FROM MYCOTOPIA!!!! cloning using dried mushrooms....yes its possible *DELETED* [Re: lightningman123]
#5808443 - 07/01/06 12:57 AM (17 years, 7 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
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Re: FROM MYCOTOPIA!!!! cloning using dried mushrooms....yes its possible [Re: Morelman]
#5809138 - 07/01/06 07:55 AM (17 years, 7 months ago) |
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Next time, let it go more than 19 days so it's fully dormant and dried. Try it with tissue a year or more old. Normally, it will take two to three weeks or more for mushroom mycelium to begin to grow from totally dry tissue. Often, growth starts sooner than that, but is almost always mold. If you have growth from completely dry tissue within a day and a half, it's almost a guarantee of mold. You can quickly determine that under a small microscope. Mushroom mycelium will be straight, while most molds will have an appearance of a steel wool pad with kinky, twisted mycelial strands with lots of side branching. Good luck.
Iodine is a great bactericide. I dip dry tissue first in iodine for a minute or two, then wash it off with a peroxide dip for five full minutes, then take the tissue through two distilled water washes before placing on the antibiotic agar. This procedure works for about a 10% success rate. Usually one or two of the cultures will grow mold, one or two will grow bacteria, one will grow mushroom mycelium, and the rest just sit there doing nothing. One of the keys for successful dry tissue recovery is to inoculate as many petri dishes as you can afford in order to use the numbers to your advantage. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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lightningman123
psilocybe boy


Registered: 06/28/06
Posts: 74
Loc: michigan
Last seen: 16 years, 7 months
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Re: FROM MYCOTOPIA!!!! cloning using dried mushrooms....yes its possible [Re: RogerRabbit]
#5809294 - 07/01/06 10:12 AM (17 years, 7 months ago) |
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whats the antibiotic agar? is it different from the pdya formula?
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
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Re: FROM MYCOTOPIA!!!! cloning using dried mushrooms....yes its possible [Re: lightningman123]
#5809706 - 07/01/06 01:07 PM (17 years, 7 months ago) |
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Quote:
lightningman123 said: whats the antibiotic agar? is it different from the pdya formula?
I use MEA with gentamicin sulphate added as an anitibiotic. Bear in mind, an antibiotic won't stop molds. It only helps a bit with bacteria. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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lightningman123
psilocybe boy


Registered: 06/28/06
Posts: 74
Loc: michigan
Last seen: 16 years, 7 months
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Re: FROM MYCOTOPIA!!!! cloning using dried mushrooms....yes its possible [Re: RogerRabbit]
#5810914 - 07/01/06 09:42 PM (17 years, 7 months ago) |
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thanks
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