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davesj1
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malt/dextrose sediment in LC with stir plate
#5810406 - 07/01/06 05:53 PM (17 years, 6 months ago) |
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If one is using a stir plate would it be beneficial to leave the sediment in the LC (from the malt/dextrose) since it will never be allowed to actually collect on the bottom? I would assume the sediment would just add to the nutrition of the LC.
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Snaggletooth
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Re: malt/dextrose sediment in LC with stir plate [Re: davesj1]
#5810418 - 07/01/06 05:57 PM (17 years, 6 months ago) |
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I dont like it, but dont know if it harmfull or not,
Though it does look like contams, that's way I go to length to filter it, with this there is no sediment.


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Omnicracker
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Re: malt/dextrose sediment in LC with stir plate [Re: davesj1]
#5810423 - 07/01/06 05:59 PM (17 years, 6 months ago) |
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i never remove the sediment, its fine, but that usually means that you are using too much malt/dext. i use 1tsp of each per pint.

also, stirplates arent kept on for 24 hours a day typically, they can be but it is unnessiccary.
Edited by Omnicracker (07/01/06 06:01 PM)
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Tippinthru
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Re: malt/dextrose sediment in LC with stir plate [Re: Omnicracker]
#5810566 - 07/01/06 06:43 PM (17 years, 6 months ago) |
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 Table spoon each light dry malt & dry dextrose, into a quart of boiling water & then filtered through 4 or 5 coffee filters into a 1/2 G jar, then PC'ed @ 15 psi 20 minutes. Day 5 today.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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KaptKid
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Re: malt/dextrose sediment in LC with stir plate [Re: Tippinthru]
#5810811 - 07/01/06 08:56 PM (17 years, 6 months ago) |
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I've done my 1st malt/dextrose LC. Nothing grew. So I will try again.
At least I'm learning.
-------------------- Child of the 60's, Tripping ever since.
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davesj1
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Re: malt/dextrose sediment in LC with stir plate [Re: Tippinthru]
#5810890 - 07/01/06 09:32 PM (17 years, 6 months ago) |
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Quote:
Tippinthru said:
 Table spoon each light dry malt & dry dextrose, into a quart of boiling water & then filtered through 4 or 5 coffee filters into a 1/2 G jar, then PC'ed @ 15 psi 20 minutes. Day 5 today.
Ive read that the idea amount was 1/2 tablespoon each per cup of water. Or 1 each per pint, which is more 1 each per quart.
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Omnicracker
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Re: malt/dextrose sediment in LC with stir plate [Re: davesj1]
#5810930 - 07/01/06 09:45 PM (17 years, 6 months ago) |
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on my last LC run, i went ahead and ran a few tests to figure out the proper amount. this is using light malt extract and dextrose without filtering:
16oz of water in a Quart jar.
Malt / Dext Results 1 Tbsp each - Way too thick, slow colonization, excess sediment 2 tsp each - Good colonization, some sediment 1 tsp each - Colonization equal to 2tsp, but no sediment. 1/2 tsp each - Healthy, but less thick, colonization.
i use 1 teaspoon of each in about 16oz of water. i see no need to filter with this amount. i get strong, sediment free LCs without wasting time filtering.
Edited by Omnicracker (07/01/06 10:35 PM)
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KaptKid
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Re: malt/dextrose sediment in LC with stir plate [Re: Omnicracker]
#5811016 - 07/01/06 10:14 PM (17 years, 6 months ago) |
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Quote:
Omnicracker said: on my last LC run, i went ahead and ran a few tests to figure out the proper amount. this is using light malt extract and dextrose without filtering:
16oz of water in a Quart jar.
Malt / Dext Results 1 Tbsp each - Way too thick, slow colonization, excess sediment 2 tsp each - Good colonization, some sediment 1 tsp each - Colonization equal to 2tsp, but no sediment. 1/2 tsp each - Healthy, but less thick, colonization.
so i use 1 heaping teaspoon of each in about 16oz of water. i see no need to filter with this amount. i get strong, sediment free LCs without wasting time filtering.
Thanks for sharing. I knew I was goig to have to start playing around with amounts. That saves a lot of time.
-------------------- Child of the 60's, Tripping ever since.
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Omnicracker
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Re: malt/dextrose sediment in LC with stir plate [Re: KaptKid]
#5811094 - 07/01/06 10:37 PM (17 years, 6 months ago) |
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i changed it slightly, try 1 LEVEL tsp / 16oz h2o.
i forgot that my LCs are actually closer to 19 ounces, so i use a heaping tsp. rock over london.
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Snaggletooth
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Re: malt/dextrose sediment in LC with stir plate [Re: Omnicracker]
#5811140 - 07/01/06 10:59 PM (17 years, 6 months ago) |
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I use 2 cups of water to,
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JSshroom
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Re: malt/dextrose sediment in LC with stir plate [Re: Snaggletooth]
#5811405 - 07/02/06 01:12 AM (17 years, 6 months ago) |
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i got the instructions from mycofile.
1 level teaspoon of each too 500-700ml of h20 in a qt jar.
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Omnicracker
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Re: malt/dextrose sediment in LC with stir plate [Re: JSshroom]
#5812094 - 07/02/06 10:54 AM (17 years, 6 months ago) |
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fastfred
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Re: malt/dextrose sediment in LC with stir plate [Re: Omnicracker]
#5815096 - 07/03/06 08:29 AM (17 years, 6 months ago) |
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3% is pretty standard. 2-4% works well. All these measurements are just confusing. Just remember 2-4%. That holds for karo, honey, malt, whatever.
For people adding dextrose to their malt media... Have you found that to be helpful?
-FF
Malt Extract Agar (3%)(MEA)(General Microbiology)(FDA M93) ---------------------------------------------------------- 30 g Malt extract 20 g Agar 1 L Distilled water
Boil to dissolve ingredients. Avoid overheating, which causes softening of agar and darkening of medium color. Autoclave 15 min at 121°C. Dispense 20-25 ml into sterile 15 x 100 mm petri dishes. Final pH, 5.5 ± 0.2.
This medium is recommended as a general maintenance medium.
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Oatman2000
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Re: malt/dextrose sediment in LC with stir plate [Re: fastfred]
#5815195 - 07/03/06 09:22 AM (17 years, 6 months ago) |
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yes... i find i like dextrose much better than adding
Malt + Karo or Malt + Honey.
i also have sediment, but give it a good swirl on the plate about once or twice a day and you'll be fine. I just made 4 different strains on 6/29 - MS inoculation.
Two of which are looking real good.
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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fastfred
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Re: malt/dextrose sediment in LC with stir plate [Re: Oatman2000]
#5815216 - 07/03/06 09:29 AM (17 years, 6 months ago) |
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You don't have to add anything to malt, it works fine on it's own. I was just wondering if people think it helps.
-FF
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Omnicracker
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Re: malt/dextrose sediment in LC with stir plate [Re: fastfred]
#5817922 - 07/03/06 10:07 PM (17 years, 6 months ago) |
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...probably not. thats just how we do it...
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