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 phrozendata
Carpal Tunnel
Registered: 04/23/00
Posts: 5,015
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 spore printing / syringe making *DELETED* 
#580710 - 03/16/02 04:26 PM (22 years, 8 months ago) |
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Post deleted by phrozendata
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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley
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 Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 19 years, 2 months
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Re: spore printing / syringe making [Re: phrozendata]
#580992 - 03/16/02 11:41 PM (22 years, 8 months ago) |
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A syringe dosent have to be "dark" to have a ton of viable spores in it. Are you using a glovebox? - I''m a big fan off the "Oven Tek" istead of using a more expensive glovebox.
In seeing that you have jusr started making syringies, you might want to let the jars sit for about three days BEFORE inoculating. That way you can throw out any jars that are not sterlizied throughly and any future contanamation would likely be the result of a bad syringie. (this can aid in the isolation of any contanamation source - be it a bad jar or a bad syringie)
' hope for the best!
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>>Jammer>>
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juggalo
member
Registered: 09/06/01
Posts: 126
Last seen: 20 years, 4 months
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Re: spore printing / syringe making [Re: phrozendata]
#580995 - 03/16/02 11:48 PM (22 years, 8 months ago) |
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heh coincidentally i just made my first syringes last nite. I won a free eq. print and ordered some syringes and last nite i boiled water, sucked up 10 cc water into 2 syringes, let them cool, poked a hole in a bag and squirted the syringes into the bag and then washed my hands and brushed the spores into the bag. the prints were stickin to the index card and my finger so i stuck my finger in the water to get the spores off my finger. I was just about to put up a post askin if anyone thought the syringes were gonna be contaminated cuz i had to stick my finger in the water but i washed my hands before doin this. Here's a pic of the syringes, can someone tell me if they look ok?
Depending on your resolution, you can click on the pick for a bigger version. My resolution is 1280x1024 so its hard to pick a good size pic.
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BeppoMarx
old hand
Registered: 09/01/01
Posts: 1,126
Last seen: 19 years, 8 months
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Re: spore printing / syringe making [Re: juggalo]
#581192 - 03/17/02 08:23 AM (22 years, 8 months ago) |
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they LOOK ok; nice and dark. with that amount of spores you could have made at least 4 total syringes though.
no way to tell if yuor finger contaminated it; theres a good chance but no way to tell until inocculation. next time skip that step of putting your finger in spore solution. unless youve PC'ed your hands for 45min lol  .
*edit*
and phrozen if you are having trouble making syringes; ill help if you want. order some prints of your choice; ship them to me; and ill make the syringes and mail them to you.
of course i will take 1/4 of the print for myself but ill make you some viable syringes.
or just buy some syringes; i just wanted to acquire some new strains 
well whatever. just trying to be helpful
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Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
BUCKETS BRIGADE left hand man!!!
Edited by BeppoMarx (03/17/02 08:30 AM)
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Anonymous
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Re: spore printing / syringe making [Re: juggalo]
#581237 - 03/17/02 09:42 AM (22 years, 8 months ago) |
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I wouldn't have stuck my finger in the water, who knows where that finger has been!
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Swami
Eggshell Walker
Registered: 01/18/00
Posts: 15,413
Loc: In the hen house
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Re: spore printing / syringe making [Re: Jammer]
#581298 - 03/17/02 11:14 AM (22 years, 8 months ago) |
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I''m a big fan off the "Oven Tek" istead of using a more expensive glovebox.
Try the Swami Garbage Bag Tek.
I get a kitchen-sized trash bag and spray full of Lysol. After I let it sit for a few minutes to kill any competitors, I perform the operation inside of the bag. I find it more sterile than the oven tek as you are fairly well protected from air currents and dropping mold spores. I respray inside before making each syringe.
Have had no contams to date.
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The proof is in the pudding.
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pleezr
fuct
Registered: 12/22/01
Posts: 1,708
Loc: NW
Last seen: 19 years, 10 months
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Re: spore printing / syringe making [Re: juggalo]
#581348 - 03/17/02 12:22 PM (22 years, 8 months ago) |
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oh no juggalo! dont use your fingers. that is a no no! i can almost garentee those syringes are toast. dont use your finger to scrap spores off, and never stick your finger in the wwater. there are many other ways to make a syringe. next time use the needle to scrape.
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pleezr
"Tell me about the fucking golf shoes!!"
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juggalo
member
Registered: 09/06/01
Posts: 126
Last seen: 20 years, 4 months
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Re: spore printing / syringe making [Re: pleezr]
#581383 - 03/17/02 01:22 PM (22 years, 8 months ago) |
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pleezr i hope to god you're wrong cuz thats the only print i got 
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 19 years, 2 months
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 Re: spore printing / syringe making [Re: juggalo]
#581859 - 03/17/02 11:49 PM (22 years, 8 months ago) |
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' dosent look good dude. You best chance might be in the faith that those syringies are soooo dark that perhaps the spores will over-power any other molds. (you might want to keep the jars in the low to mid 70's, 'cuz in the 80's the containaments will most certainly kick the spores ass in 3 days or less- if the sringies are bad)
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>>Jammer>>
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nw2shroom
shroom deity
Registered: 02/18/02
Posts: 229
Loc: Tu hablas el Spanglish??
Last seen: 17 years, 6 months
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 Re: spore printing / syringe making [Re: phrozendata]
#581875 - 03/18/02 12:09 AM (22 years, 8 months ago) |
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this is off topic but youre opinions would be great.. dont any of you all use spore prints to innoculate? I just put cambos on rye grain and like in 2 days i had growth,, so just wondering if there is a reason everybody make syringes, or was i just lucky with the lst spawn and the spores were really god?
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 19 years, 2 months
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 Re: spore printing / syringe making [Re: nw2shroom]
#581975 - 03/18/02 03:37 AM (22 years, 8 months ago) |
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That has nevered worked out for my friends. Thats awesome that it worked for you on the "lst spawn".
Man, I would be interested in the entire process (in detail) as to how you did this so eaisly. Most that I have heard of that inoculate straight from prints use the petri-dish methods.
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>>Jammer>>
Edited by Jammer (03/18/02 06:52 AM)
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nw2shroom
shroom deity
Registered: 02/18/02
Posts: 229
Loc: Tu hablas el Spanglish??
Last seen: 17 years, 6 months
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Re: spore printing / syringe making [Re: Jammer]
#582392 - 03/18/02 01:44 PM (22 years, 8 months ago) |
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simple i let the jars cool in the PC overnight, moved them to my clean box and added dropped spores, and i was surprised because the spores were given to me , he always seems to have great prints, but he was out of supplies , he usually puts it on foil then i a baggie . he had no baggies so he used apaper nd then tape it shut .in the mail most of the spores stuckj to the tape and so i had like 10 percent of the print that was useable, any one else have luck using print directly? thanks laters
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BeppoMarx
old hand
Registered: 09/01/01
Posts: 1,126
Last seen: 19 years, 8 months
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Re: spore printing / syringe making [Re: nw2shroom]
#582431 - 03/18/02 02:33 PM (22 years, 8 months ago) |
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swami- awesome idea about teh garbage bag; bet it works wonders! and cheap and easy too
never tried dry print inocculation; 0% contams with making syringes though and its very easy. 1st attemt was from a syringe that i made and all germinated. as far as teh jars go they were lost eventually being my 1st time but the syringes have always been good!
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Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
BUCKETS BRIGADE left hand man!!!
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LSD_4me
addict
Registered: 02/09/02
Posts: 416
Last seen: 22 years, 7 months
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Re: spore printing / syringe making [Re: nw2shroom]
#582455 - 03/18/02 03:04 PM (22 years, 8 months ago) |
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im sure you could have success but why... syringes work nicely and you can make a lot from one print
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Roadkill
Retired Shroomery Mod
 
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: spore printing / syringe making [Re: Swami]
#582460 - 03/18/02 03:08 PM (22 years, 8 months ago) |
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Some of us have small children.
Be careful using garbage bags.
I know.... I'm just safety oriented....lolz
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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girlskater
***meow***
Registered: 12/18/01
Posts: 260
Loc: Here
Last seen: 21 years, 3 months
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 Re: spore printing / syringe making [Re: phrozendata]
#582465 - 03/18/02 03:11 PM (22 years, 8 months ago) |
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glass rod... chopstick... as long as it's CLEAN.
fingers are not the way to go.
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Baby_Hitler
Errorist
Registered: 03/06/02
Posts: 27,895
Loc: To the limit!
Last seen: 23 minutes, 14 seconds
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 Re: spore printing / syringe making [Re: phrozendata]
#582596 - 03/18/02 05:21 PM (22 years, 8 months ago) |
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Doesn't anybody use the jar printing method?
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Not emotionally invested in the well-being of Trump supporters.
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 19 years, 2 months
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Re: spore printing / syringe making [Re: Roadkill]
#582961 - 03/19/02 01:26 AM (22 years, 8 months ago) |
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I have seen a very simular tek with a 10 gallon fish tank on it's side and clear plastic wrap covering the "top" (would be fliped so the hands can enter hortizonaly)- Lysol was also used (like 1/2 a can worth)- I have seen both perfect results and disaterious results with this "Fish Tank Glove Box Tek"- In thereoy it's pretty much the same as the Bag Tek described here. - I have seen better results with the oven tek and a little Lysol or sprayed bleach/H2o areound the oven. - Also negative ionizers have a great rep, althou they are often grose-ly over priced on drug parapenaila web sites.
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>>Jammer>>
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WakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
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Re: spore printing / syringe making [Re: phrozendata]
#583007 - 03/19/02 03:04 AM (22 years, 8 months ago) |
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In reply to:
"They always seem sticky and the needle tip and/or razor blade never seems to work and they get stuck. "
Next time you make a print,after you remove the cap, give the spores a little while to dry before you seal up the foil. It's easy to tell how much of it is dry because the moist center of the print will be darker when you remove the cap. A dry print will flake off the foil much easier. Hope that helps.
Free Spore Ring
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The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)
Edited by WakingUpLate (03/19/02 03:08 AM)
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phrozendata
Carpal Tunnel
Registered: 04/23/00
Posts: 5,015
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Re: spore printing / syringe making [Re: WakingUpLate]
#583011 - 03/19/02 03:12 AM (22 years, 8 months ago) |
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WakingUpLate:
How should I let these spores dry? Should I replace the cup used as a cover while printing and wait 24hours?
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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 19 years, 2 months
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Re: spore printing / syringe making [Re: nw2shroom]
#583017 - 03/19/02 03:23 AM (22 years, 8 months ago) |
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nw2shroom said: "simple i let the jars cool in the PC overnight, moved them to my clean box and added dropped spores"
Jammer here:
Did you follow the PF Tek for the jar preps? Are you saying that you opened the sterlized jars in the glovbox and scraped the spores on top of the 1/4" vermiculite surface? Or did you not use any vermiculite for the upper most surface of the BRF jars?
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>>Jammer>>
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WakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
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Re: spore printing / syringe making [Re: phrozendata]
#583034 - 03/19/02 03:54 AM (22 years, 8 months ago) |
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Yeah man, just like you were printing except it won't take 24 hrs. more like 4.
I don't use jars though. I use the same trays that I use for casings (from walmart) with a clear plastic lid. I double the foil around the cap leaving about a 1/2" gap around three sides. I wrap a couple of papertowels wraped in foil in the oven at 300 deg for 20 min with my print foil and tray. Then I put the foil squares and the sterile paper towel(without the foil covering) into the tray and cover it. As soon as it cools, I add the caps. The dry paper towel will draw the moisture out of the cap causing it to drop a nice dark print in 24 hrs. Sometimes I bake another paper towel half way through and replace the moist one (using oven tek).
Works great so far. Let me know if you'd like to try a couple of my prints. Hope that helps.
Free Spore Ring
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The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)
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nw2shroom
shroom deity
Registered: 02/18/02
Posts: 229
Loc: Tu hablas el Spanglish??
Last seen: 17 years, 6 months
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Re: spore printing / syringe making [Re: Jammer]
#583041 - 03/19/02 04:09 AM (22 years, 8 months ago) |
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yeah i take the lids off, i put full gloves and a gerbil cage fan w/hepa in my box, after i have use anti-bac/microbe spray , its as clean as it is gonna get, no verm , just plain rye, rye for spawn, i m using lil-sac-o-shit tek from lil shop o spores , you should check it out...
ironically the first time i tried shrooms i used syringes i made, i thought i was sterile, i think it was a dirty print , but all the jars went bad, it is wierd, maybe it is were i live, but my contams dont have visual cues, i never see a mold or anything , just a bad smell and a receding line of good fungus, i live in southern new mexico,,, go figure
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
Posts: 3,998
Loc: (God's Country) - USA
Last seen: 19 years, 2 months
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 Re: spore printing / syringe making [Re: nw2shroom]
#583045 - 03/19/02 04:19 AM (22 years, 8 months ago) |
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Shit, I'm sorry! - I just realized that you said in a previous reply that the jars were of rye grain... (not brown rice flour)
hmm... very interesting (gears turning)
If this tek works the way you say, it could drasticly cut turn around times.
Thanks for the input, I greatly apreciate it.
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>>Jammer>>
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Under_net
enthusiast
Registered: 12/08/01
Posts: 228
Loc: kentucky
Last seen: 21 years, 10 months
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Re: spore printing / syringe making [Re: Jammer]
#583046 - 03/19/02 04:29 AM (22 years, 8 months ago) |
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Here is what I have found to work really well. And I have shiteloads of spore prints stocked up. Havent had any contams yet.
I buy those little 1/4 pint / 4oz. jars. I pressure cook them along with my syringes I am re-using along with anything else I may need to pressure cook.
After everyhting has cooled down. I take the pressure cooker to my work table, which has been thoroughly cleaned with some alcohol. I always wear gloves, haircover, and dust mask.
Then i simply go to my terr and pick the best looking caps, cut them off, pick them up with a needle and immediately drop them in the jars and seal.
Within 24 hours there is usually a very dark print there(Some people say 12, but you will get a lot more spores with 24 hours)
Then when you are ready and have boiled distilled water. Simply squirt it in the small jar, shake it up and bit, and draw the goodies out.
I have only had 1 contaminated jar out of 100 so far/ knock on wood.
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karlfinn
member
Registered: 03/10/02
Posts: 162
Loc: Wild, Wonderfu,l West Vir...
Last seen: 22 years, 4 months
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Re: spore printing / syringe making [Re: Swami]
#583050 - 03/19/02 04:38 AM (22 years, 8 months ago) |
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swami-I use the same garbage bag set up, glad to see,
but I spray with H2O2 50/50 cut with water...
hands too up to my forearms...got the idea from
the old photographer film change bag of a few years ago..
although I have a flow-hood, I am out in the open kitchen,
with clean tek and my peroxide...a few hundres jars to date with
NO contams...I do my agar and master transfers in the hood....
treat my liquid mycelia innoc. with 3 ml 3% H2O2 in 2 cups broth..
before using it to expand......offered as just a suggestion...
wow---wonder if mr. finger ever read about sterile technique...??
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karlfinn
member
Registered: 03/10/02
Posts: 162
Loc: Wild, Wonderfu,l West Vir...
Last seen: 22 years, 4 months
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Re: spore printing / syringe making [Re: phrozendata]
#583057 - 03/19/02 04:51 AM (22 years, 8 months ago) |
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"I never know if i'm sterile enough."..............not this time you weren't..
even though you washed your hands there were still about a zillion
bugs on them....and into the liquid....all may not be lost if you can get some mycelia going before the contams..
you can try isolating it to a liquid culture containing peroxide to kill the nasties, if any, maybe you really LUCKED,,
then innoc. some new grain jars, with a cleaned up culture....
wear a glove next time, use sterile toolz, don't breath, and keep your appendanges outta the recipe...
what were you thinkin'? and give swami bag tek a try....
you can put a very pro hood together for about $ 150. { 24 x 12 } w/ about a 500 cfm fan.
anyway...luck to ya. hey..where did you get Malabar spores ???? Reports.
I just have not heard of that one......they're everywhere.....everywhere...I tell you.....care......farmer.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: spore printing / syringe making [Re: phrozendata]
#583535 - 03/19/02 04:17 PM (22 years, 8 months ago) |
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Most people like my prints so....here is what i do.
I take Alum foil and wipe it with Alcohol.....put it in a Zip lock Container (the big long ones with the blue lids)...they work great.
Let the Alcohol dry (if the alcohol isn't dry it will mess up the print).
Spray the hell out of the kitchen with Lysol.....My kitchen is always clean....btw.
Go get my mushrooms....cut the caps off....place the caps on a sterile cutting board.
Open the Ziplock container lid and place the mushroom cap on the foil.
Close the lid.
Wait 24 hours.....check the print.
Usually they are done....Very dark prints.
I Lysol the room again.....Open the Container take the caps out.
Close the lid and let the print dry for a day or 2.
Then I Lysol the room again.....take the prints out.
Fold them.....Mark the Alum foil with Name of the Strain....Date....and my Name.
Then i put them in a Index card file.
I have never had a complaint about my prints...just compliments
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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