Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
liquid culture experiment
    #5800590 - 06/28/06 08:02 PM (17 years, 7 months ago)

would the sulfites in just a small amount of beer prevent the growth of mycellium in a liquid culture?

I figure, if i leave a half drank bottle of beer on the counter for a week, it'll sure as hell have mold in it, so why not? anyways, i've got several different "alternative" liquid cultures going at the moment, just wondered if the sulfites (preservatives) would slow things down or possibly stop them.


--------------------


Extras: Filter Print Post Top
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: liquid culture experiment [Re: liamtheloser]
    #5800599 - 06/28/06 08:07 PM (17 years, 7 months ago)

> would the sulfites in just a small amount of beer prevent the growth of mycellium in a liquid culture?

No, but the ethanol probably would.


-FF


Extras: Filter Print Post Top
Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
Re: liquid culture experiment [Re: fastfred]
    #5800630 - 06/28/06 08:14 PM (17 years, 7 months ago)

uhhhh, i sterlized it... therefore i'd assume the ethanol would have evaporated.


--------------------


Extras: Filter Print Post Top
Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: liquid culture experiment [Re: liamtheloser]
    #5801213 - 06/28/06 10:54 PM (17 years, 7 months ago)

I've read posts where people have added beer instead of brewers yeast.

You should be good to go as long as you are using it as an additive and not the whole enchilada.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


Extras: Filter Print Post Top
Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
Re: liquid culture experiment [Re: cappa]
    #5801220 - 06/28/06 10:58 PM (17 years, 7 months ago)

yeah, i just used approx 20 ml in a half pint LC... 20 ml pabst blue ribbon, 50 ml coffee, 1 tsp karo and the rest tap water! i know ethanol wont be a problem, since the flash point of ethanol is extremely lower than water. and i boiled it for an hour, plenty of time to sterlize it.

i'll let you guys know how it turns out.


--------------------


Extras: Filter Print Post Top
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: liquid culture experiment [Re: liamtheloser]
    #5801465 - 06/28/06 11:57 PM (17 years, 7 months ago)

> uhhhh, i sterlized it... therefore i'd assume the ethanol would have evaporated.

Back to Chem 101 for you! No matter how long you boil an ethanol/water mix you will not be able to get rid of the last 5% ethanol. Beer is only around 3-7% anyway so boiling or sterilizing it isn't really going to remove much, if any, of the ethanol.

Good luck with your experiment anyway. Maybe myc will tolerate 5% alcohol.


-FF


Extras: Filter Print Post Top
InvisibleOmnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
Trusted Cultivator
Re: liquid culture experiment [Re: fastfred]
    #5801502 - 06/29/06 12:12 AM (17 years, 7 months ago)

you added coffee to a LC? i must be slipping in my old age... whats the coffee about?


--------------------




xxx..Learn Something..xxx


Extras: Filter Print Post Top
Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
Re: liquid culture experiment [Re: Omnicracker]
    #5802336 - 06/29/06 07:33 AM (17 years, 7 months ago)

lol, omnicracker: read the name of the thread :P


--------------------


Extras: Filter Print Post Top
Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
Re: liquid culture experiment [Re: liamtheloser]
    #5802340 - 06/29/06 07:35 AM (17 years, 7 months ago)

and fastfred: i never took a chemistry class, but thanks for the luck!


--------------------


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
Trusted Cultivator
OG Cultivator
Re: liquid culture experiment [Re: liamtheloser]
    #5802366 - 06/29/06 07:55 AM (17 years, 7 months ago)

I've tried beer as an lc and also as an additive to grains. The alcohol inhibits growth, and doesn't boil out.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
OfflineSickShroomer
Retired Shroom Master
Male User Gallery

Registered: 05/25/06
Posts: 361
Loc: Midwest
Last seen: 3 years, 14 days
Re: liquid culture experiment [Re: RogerRabbit]
    #5802688 - 06/29/06 10:12 AM (17 years, 7 months ago)

Why don't you try one with Non-Alcoholic Beer? Sounds like a good idea no?


--------------------
Going to miss the PNW gatherings.

I'm not here trying to make anyone feel stupid or to make myself feel good by putting ppl down. I am just here to help fellow shroomerites in there grows. I do this to keep my own knowledge fresh, and to help everyone succeed!


Extras: Filter Print Post Top
Offlinesoulsizzle
nobody f**kswith The Jesus

Registered: 05/17/05
Posts: 632
Last seen: 12 years, 5 months
Re: liquid culture experiment [Re: liamtheloser]
    #5802982 - 06/29/06 11:55 AM (17 years, 7 months ago)

Quote:

liamtheloser said:
I figure, if i leave a half drank bottle of beer on the counter for a week, it'll sure as hell have mold in it...




for the record, mushrooms are a fungus, not a mold.


--------------------


Extras: Filter Print Post Top
Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
Re: liquid culture experiment [Re: soulsizzle]
    #5804520 - 06/29/06 08:13 PM (17 years, 7 months ago)

Quote:

What is Mold?

Molds are fungi that reproduce by releasing tiny spores into the air. Spores lucky enough to land on moist objects may begin to grow. There are thousands of different types of mold and we encounter many of them every day, inside and out.




http://tuberose.com/Mold_Fungus.html


i'm sorry, just had to own someone today, turns out you were the lucky victim..


--------------------


Extras: Filter Print Post Top
Offlinesoulsizzle
nobody f**kswith The Jesus

Registered: 05/17/05
Posts: 632
Last seen: 12 years, 5 months
Re: liquid culture experiment [Re: liamtheloser]
    #5805281 - 06/30/06 12:09 AM (17 years, 7 months ago)

Quote:

liamtheloser said:
i'm sorry, just had to own someone today, turns out you were the lucky victim..




i've been grossly misinformed  :frown:  While I down't like being owned  :grin:, I appreciate the info.


--------------------


Extras: Filter Print Post Top
InvisibleLateForTheFuture
Old Hand
I'm a teapot User Gallery

Registered: 02/24/03
Posts: 845
Re: liquid culture experiment [Re: RogerRabbit]
    #5805521 - 06/30/06 01:13 AM (17 years, 7 months ago)

Quote:

RogerRabbit said:
I've tried beer as an lc and also as an additive to grains.  The alcohol inhibits growth, and doesn't boil out.
RR




:smile: All that needs to be said.


Extras: Filter Print Post Top
Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: liquid culture experiment [Re: LateForTheFuture]
    #5808099 - 06/30/06 11:39 PM (17 years, 6 months ago)

RRoger that!  :smile: Couldn't help myself.

Yeah, I had read of people using it as an additive. Never tried it myself. The non alcoholic beer sounds interesting but.. Well now that I think about it, using beer doesn't sound good in theory at all.
*ASSUMPTION* Non-alcoholic beer is regular beer put through a chemical proccess to remove the alcohol.

Making beer is largely a proccess of turning more complex sugars into simpler sugars(very simplified here but stay with me). A byproduct of this transaction is CO2.
To learn alot about the chemical proccess of making beer see:
http://www.ceic.unsw.edu.au/research/special_features/Beer.pdf

An excellent article from a chemical engineering standpoint with a dash of understandable garbage thrown in here and there!  :smile:

It stands to reason that a high CO2 level and lack of complex sugars alone could 'starve and suffocate' myc. This is based on the assumption that myc enjoys O2 and complex carbohydrates. Then we add in the alcohol factor on top of that and we have some serious ass reaming of our beloved myc. We're talking bound, gagged, and poisoned.

But this is all conjecture. RR already said what we need to know. I just thought I'd throw a few words out there in hopes of sparking up more conversation about it.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


Extras: Filter Print Post Top
Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
Re: liquid culture experiment [Re: cappa]
    #5808243 - 07/01/06 12:12 AM (17 years, 6 months ago)

actually, it's not just beer... it's beer, water and karo... and it showed signs of growth in 24 hours... so it didn't slow it down all that much. although i'll have to say that the coffee karo water mix is doing a little better.


--------------------


Extras: Filter Print Post Top
Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: liquid culture experiment [Re: liamtheloser]
    #5808253 - 07/01/06 12:14 AM (17 years, 6 months ago)

Great! Keep us posted on the results please!


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


Extras: Filter Print Post Top
Invisibleliamtheloser
Advanced Idiot
Male User Gallery
Registered: 06/07/06
Posts: 1,453
Re: liquid culture experiment [Re: cappa]
    #5808264 - 07/01/06 12:18 AM (17 years, 6 months ago)

sure thing, chicken wing!


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Pan cyan in petri dish plus giant pan & liquid culture memberjockey 1,508 4 03/02/18 10:09 AM
by meltdowner
* Liquid Cultures to Bulk Substrate Prodijal_Son 17,751 7 05/05/20 03:40 AM
by poisoned
* 1st Liquid Culture, arrrgh! Seril 14,293 10 02/28/06 08:35 PM
by Limelight_Liver
* Liquid Culture injection problem Bloomer69 2,017 5 03/14/03 01:48 AM
by BikeCourier
* Easy Liquid Culture Container Tek SixTango 20,217 7 01/17/12 04:59 PM
by dubyastep
* liquid culture failure gray1 5,941 3 07/19/01 09:37 PM
by Azure
* Starting a simple liquid culture method Garret_Hill 9,113 3 03/11/02 09:49 AM
by YourHighness
* Liquid culture problems simplemachine 3,258 10 11/12/03 08:17 PM
by deanofmean

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,178 topic views. 24 members, 224 guests and 46 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.01 seconds on 14 queries.