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InvisibleGnuBobo
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Stropharia mycelium--changing colors to "winey"
    #5800131 - 06/28/06 05:14 PM (17 years, 7 months ago)

I had some old jars of stropharia that had been colonized for about 2 weeks before I spawned them to wood.  Before this, I noticed some odd discolorations (purplish) in the jars.  Now, as the individual grains are growing out and colonizing the wood, some of the tubs have mycelium that is looking purple. 

    Is this normal?  Or is it a sign of poor health in the mycelium? 

    Thanks.  :laugh:


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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Offlinemattymonkey
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Re: Stropharia mycelium--changing colors to "winey" [Re: GnuBobo]
    #5818963 - 07/04/06 06:32 AM (17 years, 6 months ago)

Ive never seen that in any of the stropharia Ive grown, and thats now getting up to four strains in culture..

Heres some tips Ive figured out how to expand this mycelium very easily..

I had two half-gallon jars of grain that contaminated at 99%, darn! Didnt want to throw them away so I experimented... I had some sawdust that had been sitting for close to a year.. I simply wet the sawdust and layered it, about 2" of moistened sawdust, the .5" of grain(I may have taken out any contam'd kernels I saw), then another 2" of sawdust. I didnt pasteurize the sawdust or anything simply wet it. This clonized on my front porch with temps ranging from 45-65, colonization was complete in about a month.

With this success I attempted further experiments. I had another two jars of grain that did not contaminate. I also placed these in a plastic bin, one of those 58quart bins from sterilite, this time I threw-mixed instead of layering. This also grew out 100%, much quicker, though the temps on my porch started to get warmer also. I have since taken this bin and placed it into four more bins of moistened sawdust, none of this sawdust was pasteurized at all. Its growing in these four bins just fine now. I have pictures of all this but not on this comp, and don't have the internet at home. But if you are interested I can see what I can do getting the pics here.

Good luck with this amazing species..


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"listening for the secret.. searching for the sound.."


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OfflineHotnuts
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Re: Stropharia mycelium--changing colors to "winey" [Re: mattymonkey]
    #5819642 - 07/04/06 11:36 AM (17 years, 6 months ago)

I've seen my Golden Oyster spawn do the exact same thing before. I'm not sure if it's a bacteria of some sort or a drying effect of the mycelium. Proly bacteria. All of the isolates I prepare on agar do the same thing. Regardless, it's never been harmful in cultivation attempts.


Edited by hotnutz (07/06/06 11:20 AM)


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InvisibleGnuBobo
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Re: Stropharia mycelium--changing colors to "winey" [Re: Hotnuts]
    #5821694 - 07/04/06 10:36 PM (17 years, 6 months ago)

Thanks, both of you.  The mycelium seems to be shedding this purple hue and getting white as it colonizes the wood I spawned it to.  I did pasteurize the wood shavings I used, but if I do in fact spawn these to a larger wood pile (at the best I'd have around 20 gallons of colonized wood shavings), I don't think I'll pasteurize that substrate--just too big. 

So, do most people grow stropharia out indoors, or move to outdoor patches?  And if these fruitbodies are large, do they require extra depth in the substrate if you've got them in a container? 

Thanks.  :laugh:


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!


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