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Offlinekranked
it'smushtastic!!

Registered: 08/31/04
Posts: 106
Last seen: 16 years, 5 months
Partial LC success
    #5799303 - 06/28/06 12:58 PM (17 years, 7 months ago)

I prepared 6 half pint jars (250ml I believe) with one cup of water, or just under a cup, and added a tablespoon of honey to three and a tablespoon of Karo to the other three. I inoculated them in this fashion, and incubated at 82-85F. (Sterilized them before hand).

Honey - b+ 2cc
Honey - GT 2cc
Honey - Dukah Beach 2cc

Karo - b+ 2cc
Karo - GT 2cc
Karo - Dukah Beach 2cc

Both jars containing b+ had explosive growth within the first 12 hours and by the 24 hour mark had noticeably slowed down. They haven't grown anymore since, this was 5 days ago. The total level of growth wasn't even comparable to the average level I've seen around here. I considered the fact that the nutrient levels may have been depleted completely, but that doesn't make sense. None of the other jars even got going. They are still sitting in the incubator taking up space.

Taking this information, I prepared a 500mL jar with 2 tablespoons of honey and 4cc of b+. This was 3 days ago, and yet again, nothing, not even a wisp of mycelium. I can see the clusters of spores floating around in there.

So I ask, what do you think the problem might be? I can't figure it out and it's becoming extremely frustrating.


--------------------
"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945


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Invisiblecappa
Nerd
Registered: 02/12/06
Posts: 854
Re: Partial LC success [Re: kranked]
    #5801838 - 06/29/06 02:03 AM (17 years, 7 months ago)

No one is replying so here ya go.

Sugar/water ratio, gas exchange and temperature would be my first guesses.

4% sugar to 96% water, recheck your calculations and measurements.

LC's don't need incubation temps, although ~82 seems to work just fine. Dark indoor closets seem to work well for many. Anywhere indoors EXCEPT in direct sunlight or on your stove. Direct sunlight will destroy your mushroom DNA. Also, your LC could end up hotter than a fresh fucked fox in a forest fire.

LCs WILL grow with no gas exchange, up to a point, and then they stall. The gas exchange is a neccessity.


--------------------
Their are 10 types of people. Those that understand binary, and those who don't.
~Cappa.


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Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Partial LC success [Re: cappa]
    #5801922 - 06/29/06 02:26 AM (17 years, 7 months ago)

> Direct sunlight will destroy your mushroom DNA.

The glass that the jar is made from will stop the UV. Direct sunlight is probably fine, although certainly not beneficial.


-FF


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