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Offlineacidbuba
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Registered: 01/31/06
Posts: 125
Last seen: 16 years, 6 months
Liquid Culture Failure !?!?
    #5799283 - 06/28/06 12:51 PM (17 years, 7 months ago)

i made a LC last week (after reading this tek http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5238137/an/0/page/0 ) with a 1/2 pint wide mouth canning jar. i have a PC so i just boiled some water for a while filled the jar with that added some honey and a small piece of a paper clip for a stirring rod. i then PC'd if for about 20-25 min. let it sit overnight then injected a half syringe of spore solution on accident. the reason that i used a half syringe (about 5cc) was because when i made the hole for the syringe needle in the lid i sealed it with silicone so when i inserted the syringe it caused a vacuum and sucked in the content of the syringe by itself. OK so after a week of it mixing at around 80 degrees nothing really happened the water still had a yellowish hue and there was minimal growth. was it because i used to much spore solution of that the jar was air tight? i have since made a second hole in the lid that is about a 1/2 inch big and silicone a piece of tyvek over it as a breather hole. i haven't started the the process of making the new LC until i get some feedback though and the syringe that i used was good i used it in a previous successful grow... thanks in advance...


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OfflineSnaggletooth
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Registered: 10/24/05
Posts: 6,109
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Last seen: 6 years, 8 months
Re: Liquid Culture Failure !?!? [Re: acidbuba]
    #5800036 - 06/28/06 04:43 PM (17 years, 7 months ago)

I dont think it was excess of spores,

Two things, the LC might have caramelize or

The mixture was off, which will inhibit growth,

Nor was it the lack of air, since the BB tek works, but does not get that thick, do to the lack of gas exchange.

I use this for lids,




Or try the BB tek,




Though Malt/dext is by far the best I have seen,



--------------------


Atheist Chat


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