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76degrees
Apprentice

Registered: 06/08/06
Posts: 350
Loc: Middle Earth
Last seen: 17 years, 4 months
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Using Hydrogen Peroxide
#5795713 - 06/27/06 12:18 PM (17 years, 7 months ago) |
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Hi, I have heard hydrogen-peroxide mentioned when discussing liquid cultures. I was wondering if it can be cut into the water using pf tek to help reduce possibility of bacteria. I'm curious to know if and how people are using it in their cultivating practices.
-------------------- The world is changed. I feel it in the water. I feel it in the earth. I smell it in the air. Much that once was is lost, for none now live who remember it.
Edited by 76degrees (06/27/06 12:19 PM)
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Using Hydrogen Peroxide [Re: 76degrees]
#5796987 - 06/27/06 07:33 PM (17 years, 7 months ago) |
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IMHO if you're not having problems don't use peroxide. If you do have problems then it's already too late to start using peroxide. It takes the myc a couple days to recover from having peroxide being sprayed on it. You could try using lower concentrations, but then it won't be very effective. So in the end I think peroxide is pretty worthless, but I know there are plenty here who will disagree with me so YMMV. I suppose it has it's place, but it's no replacement for proper technique.
-FF
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Using Hydrogen Peroxide [Re: fastfred]
#5797068 - 06/27/06 07:53 PM (17 years, 7 months ago) |
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there's definitely no place for it in a colonizing pf tek jar. if you put it in before sterilization, it will degrade to just water and oxygen by the heat. and anything you try to add after sterilization just increases the chances you introduce contaminants... so its not a winning situation either way.
i do like a 1:10 solution (1 part 3% H2O2 to 10 parts H2O) when i dunk pf cakes. i do notice that cakes on 2nd or 3rd flushes "fizz" a lot when i place them in the water, yet interestingly they don't fizz when they're initially dunked after birth. so who knows, maybe having them exposed to the outside world for two weeks during a flush they've collected some nasties and the fizzing is the H2O2 killing it, or maybe its due to something else all together...
i use a 1:10 solution in my mister too... but i'm betting that doesn't do much... mainly because again H2O2 breaks down easy... and one of the ways to speed that up is by causing turbulence in the solution (ie, shaking). i'm wondering how much of it actually survives the nozzle on the spray bottle considering its already so dilute... but whatever, a 1L bottle is $1.50 and its peace of mind even if its a placebo. 
using it as a substitute for sterilizing substrate doesn't work very well or at all, been there done that! sure it keeps contams away those first few days, but it doesn't kill contam spores! it does the same thing to contams as it does to mycelium: slows them or prevents them from growing... until its broken down. then boom, its like you didn't do anything at all, and everything latent that was hanging out already comes back in full force.
other uses like putting it in your humidifier, PMP style fruiting chamber, TiT water, etc, are mostly pointless, because of that breakdown... unless you keep re-upping it every 2 days or so.
it is useful when applied as is at its 3% concentration to cobweb mold on casings, it will kill that on contact, but it definitely slows the mycelium down a few days until its broken down.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Using Hydrogen Peroxide [Re: creamcorn]
#5798039 - 06/28/06 12:35 AM (17 years, 7 months ago) |
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> it keeps contams away those first few days, but it doesn't kill contam spores!
Actually that's the only thing it's really good for. Peroxide IS an effective sporicide. But most fungi and some bacteria have a peroxidase enzyme which lets them live through peroxide.
-FF
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