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OfflineBigBrassBed
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Registered: 06/26/06
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Last seen: 12 years, 4 months
Microbiologist here!
    #5794113 - 06/26/06 11:50 PM (14 years, 3 months ago)

Hi there. You can call me brass. I'm a microbiologist here in the USA. My personal field of experience is in bacteria however, as that's what our company mainly deals with. I recently became interested in growing some mushrooms, and came across this site. I have access to both an autoclave as well as a laminar flow hood. I'll be doing all work after hours, obviously.

I'm figuring that my best course of action is to use 16 ounce nalgene plastic fungal culture pots. Have any of you had bad results with plasticware? I figure that i can drill a few larger holes into the lid of the pot, inoculate, and then use .2 micron filters to cover the holes, allowing for better gas exchange. Anyone forsee a problem with this setup?

I'll be using mixture of commercially available Malt Extract Broth base and PDA agar as my food source. These are very nutrient dense, and one of my concerns is with osmotic balance. Have any of you had issues of this sort?

Also, seeing as how this is my first post, I'd like to just say hello to my fellow shroomers. May your pots be plentiful.


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InvisibleTomandjerry58
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Registered: 01/27/03
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Re: Microbiologist here! [Re: BigBrassBed]
    #5794146 - 06/26/06 11:59 PM (14 years, 3 months ago)

hello...welcome to the shroomery ....good luck


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Offlinesplifner180
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Re: Microbiologist here! [Re: BigBrassBed]
    #5794149 - 06/27/06 12:00 AM (14 years, 3 months ago)

Hi Brass.

I can't answer any of your questions 'cept maybe mention RTV lids. Self-sealing, durable, cheap.

Welcome to the Shroomery. There are a lot of good people here.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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InvisibleOmnicracker
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Re: Microbiologist here! [Re: splifner180]
    #5794205 - 06/27/06 12:17 AM (14 years, 3 months ago)

so youre making a liquid culture, right? you can use almost any complex carb as a food medium for an LC. a ratio of 1.5-2tablespoons per liter of water is typical. but thats just step one, what do you have planned for substrate?


--------------------




xxx..Learn Something..xxx


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Invisibleliamtheloser
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Re: Microbiologist here! [Re: Omnicracker]
    #5794217 - 06/27/06 12:21 AM (14 years, 3 months ago)

welcome to the shroomery... there's a lot of nice people here, as splif said... but then there's the jerks like me...

just playin, have fun with your new hobby, i hope you share some of your knowledge of bacteria with us!


--------------------


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OfflineBigBrassBed
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Re: Microbiologist here! [Re: liamtheloser]
    #5794235 - 06/27/06 12:28 AM (14 years, 3 months ago)

I'll start with a little hint on managing contamination.

Keeping your media's Ph lower, around 5.5 or so, will prevent several typical bacterial contaminants from taking root. This can be done by adding dilute acid to the water for your substrate.

Thanks for the welcome everyone!


Edited by BigBrassBed (06/27/06 12:30 AM)


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OfflineSnaggletooth
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Re: Microbiologist here! [Re: BigBrassBed]
    #5794236 - 06/27/06 12:28 AM (14 years, 3 months ago)

Hey and welcome to this little community :grin:

You sound like you have a good start, and a good head on them shoulders

I doubt that most here can not answer that for ya man, but there are a few that certainly can, just might take some times until they chime in.

Starting with agar, is a great way to start, I have not yet played with it, mainly cause of no flowhood, yet :grin:

The only suggestion that I can give at this point, is to document what you can, Pics will certainly help and share it here.


Anyway man, hope you have a good grow  :thumbup:


--------------------


Atheist Chat


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InvisibleRoadkillM
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Re: Microbiologist here! [Re: BigBrassBed]
    #5794265 - 06/27/06 12:39 AM (14 years, 3 months ago)

Welcome to the Shroomery!~

I hope that you enjoy your stay!~

:smile:


I have never used any nalgene plastic...is it autoclaveable?

But I have used other types of autoclaveable plastics.
They work fine.

I'd suggest making an injection port into the lids to inoculate with.
If you have access to any vacutainers...use the cap for your injection port.
like this..

then you could use the micron filter instead of the bandaid that you see in my picture.

I use PDYA and MEA agar for culture work.
I like to switch back and forth between them when working with cultures.


If you need any help...

just ask...or do some reading.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisiblecreamcorn
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Re: Microbiologist here! [Re: BigBrassBed]
    #5794271 - 06/27/06 12:40 AM (14 years, 3 months ago)

Quote:

BigBrassBed said:
I'll start with a little hint on managing contamination.

Keeping your media's Ph lower, around 5.5 or so, will prevent several typical bacterial contaminants from taking root. This can be done by adding dilute acid to the water for your substrate.

Thanks for the welcome everyone!




yeah but low pH encourages mold growth, which is another major enemy in this hobby, and is far from ideal for fruiting. there are many types of bacteria that grow over a wide range, and a lower pH might prevent some but encourages others. i did some pH "torture tests" of my own a ways back to see what exactly mycelium would tolerate, and found that it can tolerate a rather large range, and as a side effect found bacterial infections to be consistently much more prevelant with lower pH substrates... neutral pH is a better bet all-around, and buffering to be slightly above neutral is common practice in mycology because mycelium excretes acidic metabolites.


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InvisibleRoadkillM
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Re: Microbiologist here! [Re: BigBrassBed]
    #5794274 - 06/27/06 12:41 AM (14 years, 3 months ago)

Quote:

BigBrassBed said:

I'll start with a little hint on managing contamination.

Keeping your media's Ph lower, around 5.5 or so, will prevent several typical bacterial contaminants from taking root. This can be done by adding dilute acid to the water for your substrate.

Thanks for the welcome everyone!





And check out our Contamination forum...

I'm sure you will enjoy that one!~

:smile:


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleOmnicracker
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Re: Microbiologist here! [Re: Roadkill]
    #5794324 - 06/27/06 12:57 AM (14 years, 3 months ago)

im still not clear what youre trying to do. if youre working with agar, why not use an agar dish? im sure you access to those. let us know more about what your are doing if you really want help.


--------------------




xxx..Learn Something..xxx


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OfflineSnaggletooth
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Re: Microbiologist here! [Re: BigBrassBed]
    #5794334 - 06/27/06 01:00 AM (14 years, 3 months ago)

oh yeah you can check out this link, not home made but,

https://www1.fishersci.com/Coupon?cid=1328&gid=88815&details=Y


--------------------


Atheist Chat


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Offlinemakaveli8x8
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Re: Microbiologist here! [Re: Snaggletooth]
    #5794840 - 06/27/06 05:01 AM (14 years, 3 months ago)

hmm, wounder if he is more interested in the edible feild ?


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.


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OfflineBigBrassBed
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Re: Microbiologist here! [Re: makaveli8x8]
    #5794923 - 06/27/06 06:44 AM (14 years, 3 months ago)

I'm not more interested in the edible field. I'm using agar in a very small amount to stiffen the media. If you use agar and vermiculite together, you get a very aerated, fluffy consistency. It will improve gas exchange.

I'm also making a myc. culture to inoculate my substrate. Seeing as how I have access to the aseptic techniques neccessary for such a thing, it makes more sense to me. I can easily store my culture at work without any fear.


Edited by BigBrassBed (06/27/06 06:47 AM)


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Offlinemakaveli8x8
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Re: Microbiologist here! [Re: BigBrassBed]
    #5794927 - 06/27/06 06:53 AM (14 years, 3 months ago)

well i personally think you should start out with edibles then. because doin that stuff at work, well there could be hidden cameras anywhere
anything unexpected could happen,


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.


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OfflineBigBrassBed
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Re: Microbiologist here! [Re: makaveli8x8]
    #5794934 - 06/27/06 07:13 AM (14 years, 3 months ago)

You've been doing this too long if you think there's hidden cameras in every reputable place of employment.


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Offlinemakaveli8x8
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Re: Microbiologist here! [Re: BigBrassBed]
    #5795033 - 06/27/06 09:16 AM (14 years, 3 months ago)

they sell them the size of a quarter, and its legal for bisness to tap your computers.

hidden cameras in bis. is a good idea to catch theifs that seem to be everywhere....highly highly possible.

with all these viruses being used by terror....another reason for them.


--------------------
We were sent to hell for eternity :hellfire: Ø:omgawesome:h®
We play on earth to pass the time :foreheadslap:

Over-population the root of all Evil-brings the Elites Closer to the gates.


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OfflineTheFish
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Re: Microbiologist here! [Re: makaveli8x8]
    #5795362 - 06/27/06 12:13 PM (14 years, 3 months ago)

Welcome to the boards. I envy your access to such a wonderful environment to work in. I might remodel my bathroom just to keep innoculating and spawning more fun.  :smirk:


--------------------
"Comes a time when the blind man takes your hand,
Says, 'Don't you see?'
Gotta make it somehow on the dreams you still believe.
Don't give it up.
You got an empty cup only love can fill."

Jerry Garcia


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Offlinecoda
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Re: Microbiologist here! [Re: TheFish]
    #5795913 - 06/27/06 03:41 PM (14 years, 3 months ago)

welcome to the shroomery!

A lot of people would love to gain access to the type of lab equipment you have.  Since you're already versed in microbiology cultivating mushrooms should be a snap for you. :smile:


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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InvisibleJim
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Re: Microbiologist here! [Re: Roadkill]
    #5795922 - 06/27/06 03:46 PM (14 years, 3 months ago)

those lexan nalgene bottles can take anything you throw at them....


--------------------
Use the Fucking Reply To Feature You Lazy Pieces of Shit!

afoaf said:
Jim, if you were in my city, I would let you fuck my wife.


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