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OfflineEvilGir
Im the on coming storm

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Genetic Shroom enginerring
    #578536 - 03/14/02 09:23 AM (19 years, 7 months ago)

By now shouldnt it be possible to take the dna out of a shroom that makes up psilocybe and re-sequence it in to another faste/easier growing none- psilocybe shroom or even better a fast growing plant. This would surly enable a new type of species of psychedelic plants/fungi to be created, it would be surly better than growing ears on mice.


--------------------
Fighting the man the best way I can.


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Offlinelycopodium
newbie

Registered: 03/13/02
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Re: Genetic Shroom enginerring [Re: EvilGir]
    #578546 - 03/14/02 09:42 AM (19 years, 7 months ago)

this is theoretically possible, and supposedly there is some thesis paper floating around about it although i haven't been able to read it myself to verify it's existence, let alone accuracy, but would require a tremendous amount of labor and someone with a high level of knowledge and access to a broad range of molecular bio/biochemistry equipment, for the most part, people qualified to do this type of research don't have the desire to, the time to, or wouldn't want to risk their own research/grant money/job to do illegal research.
that being said, here's what needs to be done:
the complete biosynthetic pathway for the indogenous production of psilocybin and psilocin is not yet known. this needs to be mapped and the enzymes responsible for the conversions must be cloned into expression vectors.
possibly, once all of the enzymes are known and cloned into vectors with a wide range of different antibiotic resistances, these clones could be transformed into the target cell and the expression of the enzymes could be verified via western blot, and you could do some type of analysis for the alkaloid contents and see whether they were produced through this transient expression of the biosynthetic pathway.
eventually, you would want to create a stable expression of these enzymes in a system that would be geared toward the production of alkaloids, this could mean heavily modified bacteria, transgenic tobacco, expression target to fruits like bananas (a while back there was a false rumor that a research scientist had made a transgenic orange tree that produced thc in its fruit) that is under strict control.
enough to get you started thinking?


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Offlinelycopodium
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Re: Genetic Shroom enginerring [Re: lycopodium]
    #578549 - 03/14/02 09:45 AM (19 years, 7 months ago)

i dub this research entheogenetics, you can do a search for this word but you won't find much. get rid of the double post, ask a mod to delete it.


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Offlinelycopodium
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Re: Genetic Shroom enginerring [Re: lycopodium]
    #578630 - 03/14/02 11:54 AM (19 years, 7 months ago)

here's the link to the paper, i can't download/decompress it.
if anyone can please do so and either post it in this forum or email it to me as an attachment if it's too large

http://meltingpot.fortunecity.com/gregory/1042/index.html



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OfflineSuntzu
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Re: Genetic Shroom enginerring [Re: lycopodium]
    #578637 - 03/14/02 12:03 PM (19 years, 7 months ago)

Yeah, I'd really like to see that paper; If s/he were able to get a psilo-gene into a bacterium....Very interesting for those interested in producing LOTS of alk's....overnight growth + extraction could be the equivalent of several flushes!


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OfflineskaMariaPastora
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Re: Genetic Shroom enginerring [Re: lycopodium]
    #578644 - 03/14/02 12:12 PM (19 years, 7 months ago)

If you could clone the genes responsible for psilocybin production, why would you even want to put them back into the mushroom genome? You could just insert them into bacteria and just have them produce psilocybin on a petri dish. After a few days you can just extract all the alkaloids from the bacteria colonies, and put them in a gelcap or something.

Although I'd much prefer the natural mushroom variety. The other way seems to be tampering with nature a little too much.


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OfflineSuntzu
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Re: Genetic Shroom enginerring [Re: skaMariaPastora]
    #578671 - 03/14/02 12:45 PM (19 years, 7 months ago)

This is of no practical interest to me, but even a step furthur. . .use a bacterial colony with the insert to inoculate several liters of appropriate growth broth. In a day or two at 37 degrees it would be completely turbid, depending on the promotor/transcriptional efficiency, you could have something very worthwhile to extract w/acid-alcohol.


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OfflineAnnoA
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Re: Genetic Shroom enginerring [Re: lycopodium]
    #579158 - 03/14/02 11:27 PM (19 years, 7 months ago)

You can download the self extracting file here:

http://www.fungifun.org/tampanensis_diploma.exe

It?s 2.45 Mb.


Edited by Anno (03/15/02 08:54 AM)


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Offlinelycopodium
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Re: Genetic Shroom enginerring [Re: lycopodium]
    #579670 - 03/15/02 02:45 PM (19 years, 7 months ago)

posted on poppies.org, pertaining to topic at hand



Reprinted from the Calgary Herald by Robin Summerfield

Peter Facchini is neither a drug lord nor an addict -- but a quick glance at his resume might suggest otherwise.

As the keeper of a small garden of opium poppies in an unmarked basement room at the University of Calgary, the 38-year-old Facchini has a small stash that might make a junkie's eyes glaze over.

Under 16-hour-a-day bright lights, 80 per cent humidity and 26 C temperatures of a growth chamber -- it looks like a big, green refrigerator but produces the opposite effect -- Facchini tends to his crop of the morphine-producing plants.

But as a plant cell biologist, biochemist, geneticist, molecular biologist and phytochemist, the researcher is intently focused on his plants and the potential they have in the legal world. His groundbreaking work has won international notice and national financial support.

"I'd like to see the Prairies covered by opium poppies," said a slightly hesitant Facchini. "Well, maybe not covered, but this could be a new crop alternative."

Facchini wants to isolate the opium poppy's enzyme-encoded genes, understand the 18 or so metabolic stages from beginning to codeine and then on to morphine, and then alter that process. In the end, he may wind up giving opium poppies a better reputation.

"It is very valuable as a legitimate medicinal plant, but it is also used for the illegal production of heroin, so by blocking the synthesis of the molecule at certain stages, it could be developed into a new weapon in the war on drugs," said Facchini.

"Now you would have a variety that is not only more valuable commercially -- because you're avoiding a chemical step -- but you also have a variety that avoids the possibility of converting morphine into heroin.

"In the form of codeine, it would be much more difficult to produce heroin."

Facchini's research could allow scientists to customize the pathway, possibly producing plants with more morphine. Or by stopping the process at codeine, the opium poppy could even become a Wild Rose Country crop, he suggested.

The metabolic engineering Facchini proposes may help de-stigmatize opium poppies as a crop for drug lords. Instead, it could deliver the poppies into the legal hands of Alberta farmers, provided the right, genetically altered variety are used, our laws have been amended and the public's anti-opium plant stance has changed.

Extensive competition for commodity crops such as wheat around the world have put Alberta farmers behind the eight-ball. Countries such as Argentina and China can produce crops far cheaper than farmers in Canada, he said.

With two per cent of all prescriptions filled in North America containing either morphine or codeine, a thriving market is there for the taking.

And as the largest per capita consumers of codeine in the world, Facchini said Canadians are a homegrown customer base for our farmers.

Canada gets most of its supplies of morphine and codeine from Australia and France, where opium poppies are grown legally.

Facchini's research has garnered international attention. He recently returned from France, where he is working with other researchers.

In November, the federal government awarded him a Canada Research Chair worth $1 million over the next 10 years. With that money, Facchini's salary will be covered and other money can be used to upgrade his large lab on the third floor of the biological sciences building at the university.

Six research students work with Facchini, who hopes to add about two more senior researchers in the next year.

The soft-spoken academic is quick to note he has a permit to grow the plants from the federal government.

He began studying the plant 10 years ago during his post-doctoral research at the University of Montreal. Facchini's supervisor handed him a jar full of the opium poppy seeds and told him to clone one of its genes, which had never been done.

He accomplished the task, becoming the first person to clone the gene involved with morphine biosynthesis, and there was no turning back.

Today, there is only one other lab in the world trying to understand the alkaloid synthesis of the opium plant.

"It's good to be known in a research area that's controversial," said Facchini. "Well, not exactly controversial, but it grabs people's attention."


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Offlinelycopodium
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Re: Genetic Shroom enginerring [Re: lycopodium]
    #579673 - 03/15/02 02:48 PM (19 years, 7 months ago)

still can't extract the file, even with that link anno
stoopid mac.
have you read the paper? anyway you could post it or send it to me?


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Invisiblemycofile
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Re: Genetic Shroom enginerring [Re: EvilGir]
    #580054 - 03/15/02 10:39 PM (19 years, 7 months ago)

In reply to:

By now shouldnt it be possible to take the dna out of a shroom that makes up psilocybe and re-sequence it in to another faste/easier growing none- psilocybe shroom




What's an easier to grow shroom than psilocybe cubensis? What fruits on such a wide variety of substrates, can be cased or caked, is so resilient against contams and grows so fast? I think mother nature did the entheogenetics for us and gave us cubensis!


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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OfflineEvilGir
Im the on coming storm

Registered: 11/26/01
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Re: Genetic Shroom enginerring [Re: mycofile]
    #582009 - 03/18/02 07:18 AM (19 years, 7 months ago)

The generla idea was that genetics could be used to create new strains of fungi / plants or modify existing strains to find a more simple way to obtain psilocybe. As there has not been any revenlutionary step forward since the PF method.

I think there is alway room for improvments through methods or genetics and although the PF growing methods are good I tend to find them very time consuming.
Even though learing how to do this would probable take well over 10 years +.

Also If some one was to understand how mushrooms produce psilocybe then over time it would be possible to geneticly alter fungi / plants to produce new or other substances though there own naturaly producing percursors.
An example of this would be altering the dna of morning glory plant to produce lsa into lsd or you could simple create a super shroom containing a stupid amount of psilocybe


--------------------
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Offlinelycopodium
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Re: Genetic Shroom enginerring [Re: EvilGir]
    #582187 - 03/18/02 12:02 PM (19 years, 7 months ago)

has anyone been able to extrqct/read that paper yet? if so please post details/text or email me a copy, veyr interested but can't get my mac to accept it


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Invisibleexplosiveoxygen
Prophet of TGMM

Registered: 07/10/09
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Re: Genetic Shroom enginerring [Re: EvilGir]
    #10658541 - 07/11/09 04:31 PM (12 years, 3 months ago)

If I were knowledgeable and equipped enough to do what this thread entails, I would be finding ways of producing food rather than something to free your mind.

Think about it guys.


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The Great Mycelium (TGMM) is more than you and me, we are all part of One.


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OfflineLennyk
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Re: Genetic Shroom enginerring [Re: explosiveoxygen]
    #10663481 - 07/12/09 04:29 PM (12 years, 3 months ago)

Quote:

explosiveoxygen said:
If I were knowledgeable and equipped enough to do what this thread entails, I would be finding ways of producing food rather than something to free your mind.

Think about it guys.





Welcome to the shroomery young thread necromancer :awesome:


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Mush Extract! (You can even use Vinegar!)
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Offlinebillycorgan55
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Re: Genetic Shroom enginerring [Re: EvilGir]
    #10715238 - 07/21/09 03:21 PM (12 years, 3 months ago)

i have been wondering exactly this for years...I am majoring on Genetics and i would like to become a Genetic Engineer. And perhaps, with time, this would become more fact than fiction.


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OM MADNE PADME OBIWAN LUKE HUM....


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Invisibleanonjon
Partially Right

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Re: Genetic Shroom enginerring [Re: billycorgan55]
    #10785852 - 08/02/09 03:36 PM (12 years, 2 months ago)

Hypothetically an amateur could tinker with bacteria to be introduced to the substrate that could survive pasteurization. Bacteria can exchange dna much easier than fungi.

Kinda pointless I guess tho, if one wanted better shrooms,one would work with a better species. Odds of ever accomplishing anything thru amateur experimentation seem pretty close to nil. Maybe someday someone will engineer a pan thats as durable as a cube.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


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OfflinePprPlns
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Re: Genetic Shroom enginerring [Re: EvilGir]
    #10845756 - 08/12/09 03:03 PM (12 years, 2 months ago)

This is a fun topic. Here's some links about messing with dna of bacteria  and fungus. Also, I have no knowledge of any bacteria that can create alkaloids. Usually (im no expert though) DNA has different parts, you can rearrange pieces of a portion of DNA but you can't create a piece (with the current technology). So unless bacteria have a dormant ability to produce an alkaloid hidden in their genome, no bacteria could be reprogrammed to produce psilocin. But like I said, I don't know anything about bacteria.
http://apbio.wdfiles.com/local--files/forum:thread/LabReport.pdf
http://www.fgsc.net/fgn44/weiland.html
http://www.biotech.iastate.edu/lab_protocols/DNA_Extraction_Bacteria.html


Edited by PprPlns (08/12/09 03:11 PM)


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Offlinekydelic
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Re: Genetic Shroom enginerring [Re: PprPlns]
    #10859237 - 08/14/09 03:16 PM (12 years, 2 months ago)

Another possible use would be to enable legal plants to produce the psilocybin and psilocin for a perfectly unnoticeable grow.


--------------------
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OfflineFNFAL
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Re: Genetic Shroom engineering [Re: kydelic]
    #10875439 - 08/17/09 01:52 PM (12 years, 2 months ago)

I am currently studying/working in the field of plant biotechnology. It is entirely possible to induce the uptake of foreign DNA into cells (protoplasm's) of plant tissue. Right now we have tomatoes that have the DNA of deep sea cod spliced into them which infers resistance to cold into the tomatoes. There is golden rice which has foreign DNA which contains 5x the amount of vitamin E as normal rice. The partial DNA from pathogenic bacteria has been placed in the genome of bananas, therefore those that eat them in third world countries can get their immunizations from merely eating a banana. Corn,potatoes, and other vegetables, (bt)has had natural insecticides derived from bacteria placed in its genome, thus conferring resistance to insects such as the corn borer. The list goes on and on, the work being done with genetically modified organisms truly is amazing.

In my experience when a particular chemical such as insulin is needed usually the DNA which codes for this is placed in bacteria. The bacteria can then reproduce and produce the needed compound in rates which far exceed that of plants or fungi. Huge tanks with nutrient broth (analogous to LC for fungi) is used, the chemicals are extracted in large amounts and isolated. Insulin is produced this way as are many other chemicals. Researchers have placed the genes necessary for production of silk from spiders in an attempt to extract this chemical as it is like 10x as strong as steel and like a quarter of the weight. If one were motivated to produce psilocybin I would think using a bacterium as the vector to do so would be the most efficient.

To get the DNA from one organism to another is not that difficult. I have done this with plant protoplasm tissue using basic enzymes to break down the cell walls of the plant cells then using other basic chemicals to induce re-uptake of foreign DNA. There are many ways of doing this, one can even use biolistics where the DNA is carried on small particles of gold. This is literally shot into the donor cells.

Good luck


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InvisibleRonPaulVerm
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Re: Genetic Shroom enginerring [Re: billycorgan55]
    #10889000 - 08/19/09 01:13 PM (12 years, 2 months ago)

Quote:

billycorgan55 said:
i have been wondering exactly this for years...I am majoring on Genetics and i would like to become a Genetic Engineer.




Are you trying to play God or patent life?

As far as i'm concerned genetic engineers are ruining this earth.
No offense, maybe you can enlighten me


--------------------
Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech,

Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have


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Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms 
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Invisibleguitarpah
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Re: Genetic Shroom enginerring [Re: RonPaulVerm]
    #10890056 - 08/19/09 04:27 PM (12 years, 2 months ago)

Quote:

RonPaulVerm said:
Quote:

billycorgan55 said:
i have been wondering exactly this for years...I am majoring on Genetics and i would like to become a Genetic Engineer.




Are you trying to play God or patent life?

As far as i'm concerned genetic engineers are ruining this earth.
No offense, maybe you can enlighten me



GATTICA, GATTICA!  :psycrankey:


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Invisibleanonjon
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Re: Genetic Shroom enginerring [Re: RonPaulVerm]
    #10890379 - 08/19/09 05:17 PM (12 years, 2 months ago)

I hope you're a vegetarian and ride your bicycle everywhere you go.

Are you trying to patent hypocrisy?


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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Offlinekydelic
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Re: Genetic Shroom enginerring [Re: anonjon]
    #10891853 - 08/19/09 08:30 PM (12 years, 2 months ago)

Quote:

anonjon said:
I hope you're a vegetarian and ride your bicycle everywhere you go.

Are you trying to patent hypocrisy?




And farms his own veggies, since nearly every consumed plant is GM nowadays.

I will be the first to say, though, that Monsanto is evil evil evil


--------------------
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InvisibleBlimeyGrimey
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Re: Genetic Shroom enginerring [Re: RonPaulVerm]
    #10892116 - 08/19/09 09:13 PM (12 years, 2 months ago)

Quote:

RonPaulVerm said:
Are you trying to play God or patent life?

As far as i'm concerned genetic engineers are ruining this earth.
No offense, maybe you can enlighten me




Genetic engineers are simply trying to fix God's mistakes, such as genetic diseases and the lack of food in places with arid conditions and poor soil.

I hope you don't take antibiotics when you have an infection because then you'd be playing God and trying to deviate his master plan of making you sick.


Quote:

billycorgan55 said:
i have been wondering exactly this for years...I am majoring on Genetics and i would like to become a Genetic Engineer.




God speed to you brother!

I'm majoring in Molecular Biology and minoring in Applied Biotechnology, so I understand your interest.


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Offlineapoonanor
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Re: Genetic Shroom enginerring [Re: BlimeyGrimey]
    #10895870 - 08/20/09 09:33 AM (12 years, 2 months ago)

Genetic engineers are simply trying to fix God's mistakes, such as genetic diseases and the lack of food in places with arid conditions and poor soil.

this isn't entirely true. most breeds of food crops face what's refered to as genetic depression, not from genetic defects inherent to the species but more because of continual inbreeding at the hands of man. especially in third world countries where not much is known about genetics or crop management in general. i will agree that GM has saved certain species from otherwise certain doom but it wasn't really a flawed plant that caused the problem. i'm on the side that genetic engineering and modification is essential in some fields of science, it also has to be closely monitored in other fields (for obvious reasons). i also think genetics is one of the most interesting fields in science today fwiw.


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Invisiblefastfred
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Re: Genetic Shroom engineering [Re: apoonanor]
    #10896302 - 08/20/09 11:35 AM (12 years, 2 months ago)

RonPaulVerm is trolling.  Nobody is actually that ignorant and backwards.

Please let this 7 year old thread die already.  It's been superseded by about a dozen better and more recent ones on the subject.


-FF


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InvisibleRonPaulVerm
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Re: Genetic Shroom engineering [Re: fastfred]
    #10899085 - 08/20/09 07:17 PM (12 years, 2 months ago)

I'm ain't trying to troll but I do advocate living naturally. The boundaries of that I do not know.
Blimey, I don't know what you mean about fixing God's mistakes in case you haven't noticed all of our innovation bring about new problems. And God's master plan? but I accept your views and not your slander :wink:

-I hate the idea of antibiotics for the most part considering there are other ways to get rid of or prevent infection.

-Don't know why vegetarianism is associated with eating holistically from nature. What about hunting deer? Squirrel? Animal hides, tendons, furs, bones are useful!

What I am trying to do (and don't mean to push on anyone else) is live with what nature has given us and not modify genetic make-ups of plants to the extent of human convenience. Yes people will die, be more responsible, and tougher. And maybe for once not centered around MONEY! :money:

HOWEVER, it is interesting using GM crops to fix a place that WE fucked up in the first place. Fucking it up in a way of throwing nature off balance. because somehow (and I'm trying to make sense of this) we noticed our ability to do that. We've reached this point though and can't run back.

This is turning into a PUB discussion.
peace


--------------------
Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech,

Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have


Primitive Skills                                     

Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms 
...I DO advocate learning about
Law Enforcement Against Prohibition


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InvisibleRonPaulVerm
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Re: Genetic Shroom engineering [Re: RonPaulVerm]
    #10899093 - 08/20/09 07:19 PM (12 years, 2 months ago)

If you want to see a documentary about Monsanto...



Surprisingly unbias! Very well done!


--------------------
Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech,

Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have


Primitive Skills                                     

Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms 
...I DO advocate learning about
Law Enforcement Against Prohibition


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Invisibleguitarpah
Left shroomery forever
Male

Registered: 10/13/03
Posts: 168
Re: Genetic Shroom engineering [Re: FNFAL]
    #10901832 - 08/21/09 02:16 AM (12 years, 2 months ago)

Quote:

apoonanor said:
Genetic engineers are simply trying to fix God's mistakes, such as genetic diseases and the lack of food in places with arid conditions and poor soil.




God doesn't make mistakes(cuz he's a myth) , evolution does.  Engineer the fuck out of it, just be careful.


--------------------
Can't deal with the stubborn and the ignorant.  Good bye to those who were cool.


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OfflineAgent 47
Male

Registered: 03/31/08
Posts: 631
Loc: Western PA Flag
Last seen: 2 years, 11 months
Re: Genetic Shroom engineering [Re: guitarpah]
    #10948888 - 08/27/09 04:56 PM (12 years, 1 month ago)

someone could become very wealthy with an genetic modification to assist people with the problem of cluster headaches


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Genetic Shroom engineering [Re: Agent 47]
    #10949296 - 08/27/09 06:05 PM (12 years, 1 month ago)

or grow tomatoes that produce thc...


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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Offlineylibniquah
Innerspace Explorer
Male


Registered: 05/05/09
Posts: 236
Loc: GA (unfortunately!)
Last seen: 3 months, 4 days
Re: Genetic Shroom engineering [Re: Agent 47]
    #10949924 - 08/27/09 07:43 PM (12 years, 1 month ago)

rtofl@ god's mistakes.... being perfect is the only thing that makes god god.... if god isnt perfect.... then he's just some schmuck having a wet dream while asleep at night in a parallel universe.

Quote:

Agent 47 said:
someone could become very wealthy with an genetic modification to assist people with the problem of cluster headaches




Word.
I myself suffer from Cluster Headaches, and I wouldn't wish this on anyone. People don't understand just how severe they are.


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Offlinebillycorgan55
Stranger

Registered: 11/14/08
Posts: 537
Last seen: 9 years, 5 months
Re: Genetic Shroom engineering [Re: ylibniquah]
    #10950372 - 08/27/09 09:07 PM (12 years, 1 month ago)

i saw a documentary on people with cluster headaches. They say it is so

severe that it has caused a numerous amount of people to actually kill

themselves so they could escape the pain. I myself suffur from migrain

headaches but every day i thank evolution that I dont get cluster

headaches


--------------------
OM MADNE PADME OBIWAN LUKE HUM....


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OfflineAgent 47
Male

Registered: 03/31/08
Posts: 631
Loc: Western PA Flag
Last seen: 2 years, 11 months
Re: Genetic Shroom engineering [Re: ylibniquah]
    #10951422 - 08/27/09 11:36 PM (12 years, 1 month ago)

Quote:

ylibniquah said:
Word.
I myself suffer from Cluster Headaches, and I wouldn't wish this on anyone. People don't understand just how severe they are.




I don't know first hand the pain, but a good friend of mine suffers from them and it's obvious the agony it causes.

Quote:

billycorgan55 said:
i saw a documentary on people with cluster headaches. They say it is so

severe that it has caused a numerous amount of people to actually kill

themselves so they could escape the pain. I myself suffur from migrain

headaches but every day i thank evolution that I dont get cluster

headaches




Yup, they are sometimes referred to as "Suicide Headaches"


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OfflineAlien Hybrid
Registered: 09/02/09
Posts: 1
Last seen: 8 years, 7 months
Re: Genetic Shroom engineering [Re: FNFAL]
    #10983344 - 09/02/09 12:54 AM (12 years, 1 month ago)

Just a thought! Would it be possible to extract all the goodies from the mushroom and add them into a hydroponic nutrient solution for growing cannabis? I was reading an article about adding flavored extracts to the nutrient solution to get a desired flavor and I started thinking, what else could be added?


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Offlinelsatrap
Mush maker
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Registered: 08/07/05
Posts: 1,156
Loc: S.E USA
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Re: Genetic Shroom engineering [Re: Alien Hybrid]
    #10984765 - 09/02/09 08:39 AM (12 years, 1 month ago)

I to suffer from clusters. I've been on so many different meds in the past it was insane. I finally tried using indole ring hallucenegens and have stopped my Cluster cycles for a few months at a time.. I have to re- dose every once in a while. So I must say I'm so thankful for shrooms.It would be nice if there was a med that was made from them.


--------------------
At this moment you should be with us, feeling like we do.


               


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Offlineapoonanor
Time & Space Traveler


Registered: 04/20/09
Posts: 380
Last seen: 7 years, 5 months
Re: Genetic Shroom engineering [Re: lsatrap]
    #10984831 - 09/02/09 09:07 AM (12 years, 1 month ago)

I was reading an article about adding flavored extracts to the nutrient solution to get a desired flavor and I started thinking, what else could be added?

having extensive knowledge in this area, i can easily say, (though it's a little off topic) adding flavoring to cannabis nutrients will NOT add to the flavor of the finished product.


Edited by apoonanor (09/03/09 03:59 PM)


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OfflineShroomFrog
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Registered: 09/03/09
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Re: Genetic Shroom engineering [Re: apoonanor]
    #10990591 - 09/03/09 03:57 AM (12 years, 1 month ago)

If someone was to engineer an organism to utilize the psilocybin pathway.  Once the appropriate genes are isolated, they could be inserted into a plasmid with a promoter for something like lactose or other sugar like mannitol.  The native promoter might even be sufficient depending on how it's activated.  I admit I know little concerning the full mechanics of this pathway. 

The plasmid if small enough could be used to transform bacteria and induced and later extracted.  Or for more ease of production (or if the pathway cannot be fit into a single plasmid), a yeast artificial chromosome could be developed with similar promoters and insert into Saccharomyces cerevisiae which wouldn't need extraction.

Plasmid and YAC's are easier to develop than isolating the metabolic pathways anyway.


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Offlinememory_nirvana
nomad
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Registered: 08/03/09
Posts: 288
Loc: oklahoma
Last seen: 8 years, 8 months
Re: Genetic Shroom engineering [Re: FNFAL]
    #11038501 - 09/11/09 04:42 AM (12 years, 1 month ago)

this is all i have been able to find on the subject

The Original "Super Mushroom," Agaricus blazei

For decades, anthropologists and medical researchers puzzled over the fact that the people of Piedade, a small mountain town in the humid rainforest of Brazil, lived to extraordinary age, many well into their 100s.

They were almost never bothered by serious illnesses like heart disease, arthritis, high blood pressure, diabetes, cancer, hepatitis, or Alzheimer's.

For years, scientists attributed this incredible health and longevity to some powerful genetic defense, passed from one generation to the next. Was it

An anti-cancer, longer-life gene? No. . .something even better!

But in the 1960s, a Japanese scientist by the name of Furumoto Takatoshi discovered the true secret, a rare strain of a certain mushroom native to the local mountains of the Piedade area. It turned out that the locals used this "sun mushroom" or "mushroom of life," as they called it (known botanically as Agaricus blazei Murill fungus) to make a popular medicinal tea. It was also a regular ingredient in their cooking!

Furumoto's research established an unequivocal connection between the mushroom and the longer life and extraordinary good health of the people who consumed it.

Since then, dozens upon dozens of scientific studies have analyzed the complex organic compounds that account for the powerful, anti-aging, perfect health, longer-life properties of this particular, very rare strain of mushroom and confirmed that Agaricus blazei is indeed the source of longevity and extraordinary good health enjoyed by the people of Piedade.

Therefore, it was not "good genes," but rather the miracle mushroom that protected these people against heart attack, stroke, diabetes, arthritis, high blood pressure, cancer, premature aging and most of the other illnesses that plague the rest of the world.

That is good news, because, while you can not do anything about your genetic makeup, you can benefit from the longer-life nutrients of this amazing, miracle mushroom, Agaricus blazei. In fact, many already have, including ex-President Ronald Reagan who, according to the media, used it to treat and cure his skin cancer.

No wonder the miracle mushroom Agaricus blazei was heralded as the long-awaited solution to cancer.

Only there were TWO big problems!

Unfortunately, this particular strain of the mushroom grows only in the unique soil and climate conditions found around the village of Piedade, and repeated attempts at commercial cultivation failed. Although the original cultures that Furumoto sent to Japan have been painstakingly maintained and isolated to guard their genetic purity, for years, the only, very limited source was the rainforest of Piedade.

And that area, like so much of the Brazilian rainforest, has become urbanized and industrialized since the mushroom's discovery, some 40-60 years ago. Nowadays, you would be hard pressed to find the "super mushroom" growing wild.

A near tragedy!

Of course, the original discovery that this amazing, miracle mushroom provided powerful anti-cancer, immune-boosting protection created huge interest in the scientific community.

For years, front-line research laboratories and world-class bio-chemical researchers have been studying the many different strains of the Agaricus blazei mushrooms found throughout the world.

While none has been identified with a polysaccharide profile that exactly matches the Piedade Agaricus blazei, herbalists working in the U.S. recently discovered that a related Agaricus strain does contain most of the active bio-nutrients that make the Brazilian mushroom so powerful.

That was especially good news, because this newly researched, still-un-named strain of Agaricus mushroom contains more than a few powerful polysaccharide nutrients that are uniquely its own. Not only do these nutrients possess powerful better-health properties, they complement those of the original Brazilian strain.

The best news of all is that geneticists have now succeeded in crossing genetically pure cultures descended from the original Piedade mushroom taken to Japan, with the newly researched complementary strain, to create a new, hybrid Agaricus mushroom that is even more powerful than either of its "parents!"

Science introduces the new Hybrid Mushroom . . . the most powerful super-mushroom of all!

Laboratory analysis and exhaustive clinical tests prove that the unique combination of some 85 nutrients found only in this miracle hybrid mushroom, including the powerful, complex polysaccharide, â-D-Glucan can:

Destroy a variety of deadly cancer cells (without side effects)
Guard against deadly heart disease and crippling stroke
Slash your risk of ever developing diabetes
Control high blood pressure
Inhibit or reverse arthritis
Protect against Alzheimer's
Boost immune function to guard against and a host of other illnesses
Correct digestive and weight problems
Boost energy
Protect against liver and kidney disease
Protect against hepatitis
Ease the pain and stiffness of arthritic joints
Promote natural, more-restful sleep
Help you look and feel 20 years younger.
While this hybrid strain has been analyzed by biochemists and nutritional researchers, its potency has been appreciated for many years by the few who have been acquainted with it:

The Iron Chef of Japan only serves Kobe beef that has been fed with this mushroom.
The Royal Family of Saudi Arabia feeds this mushroom to their champion thoroughbreds.
The most powerful, patented, secret-to-eternal-health superfood in the world!

The amazing, SUPER hybrid mushroom combines the same, historical, extensively studied nutrients found only in the heirloom strain of the Agaricus blazei mushroom (first studied by Takatoshi Furumoto more than 40 years ago). With the complementary nutrients in newly researched strain to provide the astonishing, immune boosting ... cholesterol lowering ... better health. . .longer life benefits of both of these two miracle mushrooms in the breakthrough hybrid. There is simply nothing else in the world to equal it!

The DNA for the new hybrid mushroom is so valuable it is kept in two separate vaults in the United States and Europe to protect its chemical makeup.
The potency of the hybrid mushroom is protected by a strictly controlled, strictly organic, growing process that provides a consistent temperature, soil, watering, and quarantined environment
Every yield of the hybrid mushroom is compared to the original to be sure the DNA from the two components matches for efficacy and potency.
No other super-food comes close!

The hybrid mushroom extract contains large quantities of â-D-Glucan (Polysaccharide); clinically proven to act as an immunological activator that enhances the immune system.

The powerful nutrients in this patented supplement also stimulate natural killer cells, whose job is to defend the body by responding as soon as damaged (as in cancer) cells appear, while other immune cells gear up for action. Natural killer cells are best known for their capacity to kill tumor cells before they become established cancers.

Here is how this miracle formula works in your body to help you live a longer healthier life:

â-D-Glucan and other powerful nutrients enhance the activity of macrophage, an antibody cell that destroys or delays the proliferation of cancer cells.
It contains natural steroids, known for their anti-cancer effect.
It contains large amounts of non-digestive dietary fibers that absorb and discharge cancerous materials in your body.
It reduces the blood glucose (sugar) and guards against diabetes.
It acts to reduce blood pressure, cholesterol and arteriosclerosis.
It can also enhance the effect of Chemotherapy. The hybrid mushroom extract is all-natural and there are no known side effects from taking it.
Everybody can expect intensive improvement and prevention of serious illness without risk and fear through immunotherapy. Now you too can benefit from the same powerful longer life, disease stopping nutrients that, study after study, have shown account for the phenomenal long life and good health of the native people of Piedade, who for hundreds of years have made it a regular part of their diet.


--------------------
NOT ALL WHO WANDER ARE LOST



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Offlinelsatrap
Mush maker
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Registered: 08/07/05
Posts: 1,156
Loc: S.E USA
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Re: Genetic Shroom engineering [Re: memory_nirvana]
    #11039415 - 09/11/09 11:32 AM (12 years, 1 month ago)

Now you too can benefit from the same powerful longer life, disease stopping nutrients that, study after study, have shown account for the phenomenal long life and good health of the native people of Piedade, who for hundreds of years have made it a regular part of their diet.


  How can we get a hold of this mushroom? Is it available to the public?
I find this very interesting.


--------------------
At this moment you should be with us, feeling like we do.


               


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OfflineLucienis
Learning Permit
Registered: 08/16/09
Posts: 98
Last seen: 9 years, 4 months
Re: Genetic Shroom enginerring [Re: explosiveoxygen]
    #11065280 - 09/15/09 07:22 PM (12 years, 1 month ago)

Quote:

explosiveoxygen said:
If I were knowledgeable and equipped enough to do what this thread entails, I would be finding ways of producing food rather than something to free your mind.

Think about it guys.




There exists enough food in this world to feed every single living person a healthy and well balanced diet. It is not the scientist's fault that people are starving, go blame the politicians and businessmen.


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Offlinelsatrap
Mush maker
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Re: Genetic Shroom enginerring [Re: Lucienis]
    #11065297 - 09/15/09 07:26 PM (12 years, 1 month ago)

Quote:

Lucienis said:
Quote:

explosiveoxygen said:
If I were knowledgeable and equipped enough to do what this thread entails, I would be finding ways of producing food rather than something to free your mind.

Think about it guys.




There exists enough food in this world to feed every single living person a healthy and well balanced diet. It is not the scientist's fault that people are starving, go blame the politicians and businessmen.



  Well said


--------------------
At this moment you should be with us, feeling like we do.


               


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InvisibleRonPaulVerm
Piss Christ
Male

Registered: 07/21/08
Posts: 1,072
Re: Genetic Shroom enginerring [Re: Lucienis]
    #11066106 - 09/15/09 10:16 PM (12 years, 1 month ago)

Agreed
Quote:

Lucienis said:
Quote:

explosiveoxygen said:
If I were knowledgeable and equipped enough to do what this thread entails, I would be finding ways of producing food rather than something to free your mind.

Think about it guys.




There exists enough food in this world to feed every single living person a healthy and well balanced diet. It is not the scientist's fault that people are starving, go blame the politicians and businessmen.





AGREED!
However, if you watch that documentary i posted on last page about Monsanto you'll see (and I hope it strikes people) that:
The GMO Scientists (the very least in monsanto) are also in the FDA and the Judicial Branch!!!!!!!!!!!!!!!!!!
WTF!!!!!!

Conflict of interest anyone?

Justice Clarence Thomas, appointed under Bush Sr. was a big time attorney for Monsanto. Once in the Supreme court he signed off the document Monsanto needed to start patenting life...:mad2:

I just don't know how to stop it.....the conflict of interest between gov't and big business is the definition of Fascism I do believe


--------------------
Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech,

Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have


Primitive Skills                                     

Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms 
...I DO advocate learning about
Law Enforcement Against Prohibition


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Invisiblestedenko
Stranger
Male User Gallery
Registered: 10/13/08
Posts: 756
Re: Genetic Shroom enginerring [Re: RonPaulVerm]
    #11068304 - 09/16/09 07:22 AM (12 years, 1 month ago)

GMO people.

Is it too much to hope for they all get hit by a bus?

GMO is bad bad bad

Monsanto will own you all if you keep it up.


--------------------
Ratings are bullshit, opt out...
http://www.shroomery.org/forums/showflat.php/Number/10525736#10525736


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OfflineAgent 47
Male

Registered: 03/31/08
Posts: 631
Loc: Western PA Flag
Last seen: 2 years, 11 months
Re: Genetic Shroom enginerring [Re: RonPaulVerm]
    #11069478 - 09/16/09 01:13 PM (12 years, 1 month ago)

Quote:

RonPaulVerm said:
Agreed
Quote:

Lucienis said:
Quote:

explosiveoxygen said:
If I were knowledgeable and equipped enough to do what this thread entails, I would be finding ways of producing food rather than something to free your mind.

Think about it guys.




There exists enough food in this world to feed every single living person a healthy and well balanced diet. It is not the scientist's fault that people are starving, go blame the politicians and businessmen.





AGREED!
However, if you watch that documentary i posted on last page about Monsanto you'll see (and I hope it strikes people) that:
The GMO Scientists (the very least in monsanto) are also in the FDA and the Judicial Branch!!!!!!!!!!!!!!!!!!
WTF!!!!!!

Conflict of interest anyone?

Justice Clarence Thomas, appointed under Bush Sr. was a big time attorney for Monsanto. Once in the Supreme court he signed off the document Monsanto needed to start patenting life...:mad2:

I just don't know how to stop it.....the conflict of interest between gov't and big business is the definition of Fascism I do believe




Ah nice, a Ron Paul supporter. Your probably also aware of the bill going around trying go from the great idea to limit, or stop corporate support to government officials, to allowing, and supporting corporate sponsorship. Stephen Colbert covered it lightly last night, I was excited to see that even a comedic, sarcastic, journalist was able to comprehend the devastation brought upon by this terrible bill. I'm doubting that it will get passed, but you never know with the people in charge today.


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