Can contaminated spore water be saved? - Mushroom Cultivation

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mesq
Psychonaut

Registered: 02/24/02
Posts: 284
Last seen: 9 years, 6 months

Can contaminated spore water be saved?

#578324 - 03/13/02 11:56 PM (23 years, 20 days ago)

Hey I think this is my first post...ermmm hi to all the guys and gals i have talked to on #shroomery

I received some spore water from a friend and he says that he tested it shortly after sending it and that it is contaminated.... I trust him cos well he should know.. he made it, so is there any way i can keep the spores and kill any contams>??

at the moment the jars of spore water are sitting in my crisper waiting for something to be done....even if it is to bin it..

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Anno
Experimenter

Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 18 days, 22 hours

Re: Can contaminated spore water be saved?

[Re: mesq]
#578339 - 03/14/02 12:07 AM (23 years, 20 days ago)

>so is there any way i can keep the spores and kill any contams
Thre isn?t anything easily feasable you could do with it.

--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

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MAIA
World-BridgerKartikeya (DftS)

Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 2 months, 9 days

Re: Can contaminated spore water be saved?

[Re: mesq]
#578503 - 03/14/02 06:04 AM (23 years, 20 days ago)

I think it's not worth it.
Anyway, you could give agar a shot and then make several transfers until you got healthy isolated growth, got to have some experience though.

Peace,
MAIA

--------------------
Spiritual being, living a human experience ... The Shroomery Mandala

Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire

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Seuss
Error: divide byzero

Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 1 month, 6 days

Re: Can contaminated spore water be saved?

[Re: mesq]
#578636 - 03/14/02 10:03 AM (23 years, 19 days ago)

I spent a lot of time (several months) trying to salvage a bad print to test out my skills. It can be done but is very very difficult and not worth the time or expense. I would make an agar plate and transfer the contaminated spores. Once I got some mycelium+contamination growth going, I would transfer the cleanest looking area to a new agar plate. I have access to a very nice jacketed incubator and could tweak the environment (co2 and rh) to try and help the cubensis myclium while making it harder for the contamination to grow. I also had access to a nice microscope to view the plates and verify the existance of contamination. I had lots and lots of failure before I finally isolated clean mycelium. The amount of agar I went though would have easily paid for a new spore print... My conclusion was although fun, not worth the time or money.

--------------------
Just another spore in the wind.

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Karen
Pooh-Bah

Registered: 01/02/00
Posts: 1,677
Last seen: 7 years, 10 months

Re: Can contaminated spore water be saved?

[Re: mesq]
#579214 - 03/14/02 10:48 PM (23 years, 19 days ago)

If your spore water is contaminated it couldn't hurt to squirt it outside if you live in the right climate. It probably won't do anything but some people have had some luck with it.

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rungi
journeymana

Registered: 07/11/01
Posts: 212
Last seen: 16 years, 2 months

Re: Can contaminated spore water be saved?

[Re: Karen]
#579703 - 03/15/02 01:24 PM (23 years, 18 days ago)

i read somewhere you can suspend the spores in water and then filter the water first with a micron filter just a a little smallle and than use a filter just a little larger than the spore size.

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