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OfflineMrJingles
journeyman
Registered: 03/13/02
Posts: 53
Last seen: 15 years, 7 months
Four days to colonise!!
    #578241 - 03/13/02 11:45 PM (15 years, 9 months ago)

Hi everyone!
I'm quite a newbie at cultivation. I've tried many times to cultivate the mycelium, but have always had contamination probs, before I could get fruit. This is my latest attempt, and I need some advice as to what is occuring ....

Thailand-Koh Samui Cubensis spore print (Hawk's Eye)

THE COOKERY

I started on the Wednesday, by cooking.....
6 cups of Brown Rice (whole)+3 cups of water
In a flat, microwave proof, 20x30cms, rectangular tub, (airtight),
for aprox. 30 mins.

The rice cooked without stiring, and all contaminants were killed.

Taking the tub out after a few hours to cool down, I made sure the lid was clipped down firmly, BEFORE I took it from the microwave, and placed it in the fridge to aid cooling.

After another few hours I removed the tub.

Getting the Spore print ready first, I then removed the lid and placed it face down on a disinfected surface.

I then quickly scraped, what would have been, millions of tiny spores STRAIT ONTO the surface of the cooked rice.

I quickly put the lid back on AIRTIGHT, and placed it in a cupboard.



THE AMAZEMENT!!

I checked it every day.. Watching for spots(contaminents).

I couldn't believe it.

By the Saturday (day 3!!!), the mycelium had started to grow.

By the Sunday, the mycelium had taken over 30% of the surface (30% of 20x30cms!!)

Don't forget, thats THREE days since inoculation with DRY SPORES!!

Day five,(Monday) I noticed a slight discolouration...
It was a patch of green mould!!!!!
I raced down to the Chemist, and bought a bottle of H2O2, and injected the spot at different depths, hoping this would kill the bug.

Day Seven, the spot seems to have stoped growing and is on the retreat!! And the mycelium has just about taken over most of the surface..!! Today I will transfer it to the grow chamber..

Day Eight, (Thursday) The grow chamber seems to be working well, but I've noticed that the substrate, in the plastic tub, is obtaining a mushy consistance, (to my knowledge this is bad..) So I've placed the substrate (85% colonised) strait onto the perlite floor.
I hope to gain some benificial drainage from the porus stone, thus solving the mushy substrate problem.
I also sprinkled about 1/2 inch of moist perlite over the top. (wetting done with H2O2/water mix)
Cross the fingers. The green stuff has died, and even become all burnt looking, with no other spots appearing..

Day Ten, The Mycelium has recovered and is showing growth on the new casting. I'm starting the light and ventilation today also.

Day Fourteen, The mycelium is permeating the perlite, but no pins as yet. I'm guessing that the network is not established enough to produce pins yet. From my reading, I gather it's still only early days tho. I hope to see some sort of change soon. No contaminants yet!! I'm thinking my H2O2+H2O sloution, is doing it's job!

Can anyone speculate as to whether the dead contaminant would be responsible? Or whether I'm just to eager to see fruit bodies yet?

For the record, I live on the West Coast of Australia, and no heating has been needed as it's summer time here. (20 to 30degCel)


--------------------
Giggidy giggidy boom boom boom


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OfflineDrAbeLincoln
enthusiast

Registered: 08/31/01
Posts: 248
Last seen: 13 years, 7 months
Re: Four days to colonise!! [Re: MrJingles]
    #578295 - 03/14/02 01:14 AM (15 years, 9 months ago)

I dont think you've got mycelium...spores need at least 12 hours to rehydrate before being used as an innoculent...just follow the PF tek.....what you have going right now sounds like a perfect reciepe for disaster


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OfflineMrJingles
journeyman
Registered: 03/13/02
Posts: 53
Last seen: 15 years, 7 months
Re: Four days to colonise!! [Re: DrAbeLincoln]
    #578323 - 03/14/02 01:54 AM (15 years, 9 months ago)

I have indeed cultured Mycelium before, and have absolutely no doubt that it is indeed mycelium. It is white and very whispy, as a KS should be. It has permiated the light casing I have put on it.. I'm just waiting for knots.
But is indeed Mycelium.
:-)


--------------------
Giggidy giggidy boom boom boom


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OfflineDrAbeLincoln
enthusiast

Registered: 08/31/01
Posts: 248
Last seen: 13 years, 7 months
Re: Four days to colonise!! [Re: MrJingles]
    #578377 - 03/14/02 03:08 AM (15 years, 9 months ago)

I still say reciepe for disaster....squirting some h202 on green mold that is already present will do close to nothing...h202 is good for killing spores not pre existing molds..I'd reread the pf tek and start over using the pf-tek..


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InvisibleSumGuy
addict

Registered: 07/26/01
Posts: 556
Re: Four days to colonise!! [Re: MrJingles]
    #578529 - 03/14/02 09:07 AM (15 years, 9 months ago)

white and very whispy sounds like cobweb mold but that's just one guy's opinion .. i think that you're not going to get mushrooms that way and if you do i think you should post pictures here ... maybe post some anyway in the contamination forum


--------------------
-SumGuy :cool:


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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 11 months, 6 days
Re: Four days to colonise!! [Re: MrJingles]
    #578608 - 03/14/02 11:20 AM (15 years, 9 months ago)

I don't mean this to be a flame, but rather to help you figure out what went wrong...

> have always had contamination probs
> microwave proof rectangular tub for aprox. 30 mins
> The rice cooked without stiring, and all contaminants were killed.
> Day five, a patch of green mould!!!!!

You really need to use a pressure cooker to ensure that all contaminats are killed. The microwave method will kill off most forms of contamination, but there are still several out there that can sit in the microwave all day and wave back happily.

> Or whether I'm just to eager to see fruit bodies yet?

If you temps, humidity, moisture level, and air exchange are all good then you probably just need to wait a bit.


--------------------
Just another spore in the wind.


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Invisiblemrdasani
enthusiast
Registered: 10/19/01
Posts: 224
Re: Four days to colonise!! [Re: Seuss]
    #578706 - 03/14/02 01:14 PM (15 years, 9 months ago)

Those whole grains need to be pressure cooked. And it isn't nice for you to keeep checking it everyday to see if there is any growth by opening up the lid. Unless you do this in a glove box. But I can't believe that your actually trying this.

If it works, its pure luck. Thats the truth. Because it has so much exposure to natural air everytime u check ur mix. And spray h2o2 isn't gonna help. Its not like that'll just magically make the green mold dissapear. It might even spread some airborne spores of the greeny to other clear (uncolonized) parts of the bin.

Try looking at investing some supplies.


--------------------
"Come on you raver, you seer of visions,
come on you painter, you piper, you prisoner, and shine!" - Pink Floyd (Wish You Were Here 1975)

"Never underestimate the power of denial." -from American Beauty.


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OfflineMrJingles
journeyman
Registered: 03/13/02
Posts: 53
Last seen: 15 years, 7 months
Re: Four days to colonise!! [Re: mrdasani]
    #579311 - 03/15/02 02:40 AM (15 years, 9 months ago)

The Green has dissapeared and hasn't returned AT ALL. I have had that rice in the fruiting chamber for over two weeks now, and not bad odours have occured to this day, and through checking in the pictures forum, Im certain that the white fuzz is most deffinately KS mycelium. I have read that manual airing is fine, once the mycelium is colonised, so I have been doing just that.
Rather than the pessimistic pionts of view, I'd appreciate some constructive advice as how to get the mycelium to knot and fruit WITHIN a growth chamber.. putting it in the fridge isn't an option.
All your feedback is appreciated, but try and HELP me please....
thanx
I have just started a cake method also.. The microwave is great, as I'm using plastic containers with screw top lids. They produce considerable pressure on the substrate whilst cooking.. I will persevere.. :-)
I do not have access to a digital camera as yet, but I will be sure to post pics once I can.


--------------------
Giggidy giggidy boom boom boom


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OfflineMrJingles
journeyman
Registered: 03/13/02
Posts: 53
Last seen: 15 years, 7 months
Re: Four days to colonise!! [Re: MrJingles]
    #587763 - 03/24/02 02:27 AM (15 years, 8 months ago)

Day 21, after inoculation.......
Houston... we have pins........ WOOOO HOO!


--------------------
Giggidy giggidy boom boom boom


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Offlinefleshofgods
SpiritualVisionary
Registered: 03/13/03
Posts: 444
Loc: In a daze . . .
Last seen: 13 years, 6 months
Re: Four days to colonise!! [Re: MrJingles]
    #1436774 - 04/07/03 06:36 PM (14 years, 8 months ago)

Quote:

Day Eight, (Thursday) The grow chamber seems to be working well, but I've noticed that the substrate, in the plastic tub, is obtaining a mushy consistance, (to my knowledge this is bad..) So I've placed the substrate (85% colonised) strait onto the perlite floor.



if i had a soggy cake, i sure as hell wouldnt put it directly on my moist pearlite. It will just absorb even more water, not get rid of it.


--------------------
Holler!


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OfflineDim
The Embodimentof the Conceptof Fuck

Registered: 03/30/03
Posts: 34
Loc: some terrifying, cold, im...
Last seen: 14 years, 4 months
Re: Four days to colonise!! [Re: fleshofgods]
    #1437304 - 04/07/03 09:14 PM (14 years, 8 months ago)

dude, don't eat the mushrooms. If they had mould growing near them, and the mould is one that produced toxins, then the mushrooms will have absorbed them into their cell network. You have no way of knowing if the mould was benign or not. You could easily die from bad microbes, or go blind or something like that. Not to discourage you or anything, but you might wanna follow the pf tek more religiously.


--------------------
"Once a nation bases its security on an absolute weapon, such as the atom bomb, it becomes psychologically necessary to believe in an absolute enemy." - P.M.S. Blackett


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Invisiblegeorge castanzaM
Lord Of The Idiots!
 User Gallery Arcade Champion: BurgerTime, Space Invaders
Registered: 10/21/02
Posts: 7,459
Trusted Cultivator
Re: Four days to colonise!! [Re: Dim]
    #1437382 - 04/07/03 09:47 PM (14 years, 8 months ago)

the koh samui strain is amazingly fast.
kramer says that risking your health to get a buzz is your decision
athough he is not even that crazy or is he?

good luck!


--------------------



     
Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Offlinefleshofgods
SpiritualVisionary
Registered: 03/13/03
Posts: 444
Loc: In a daze . . .
Last seen: 13 years, 6 months
Re: Four days to colonise!! [Re: george castanza]
    #1440586 - 04/08/03 09:50 PM (14 years, 8 months ago)

Nice avatar DIM! A Clockwork Orange?


--------------------
Holler!


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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Four days to colonise!! [Re: fleshofgods]
    #1443555 - 04/09/03 05:21 PM (14 years, 8 months ago)

must be a fan of Stanley Kuberic....I have the movie....on Laser Disk from 1981...cant watch it now because nothing plays a 12inch CD...

P#1


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