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fahtster
Now With 33%More Faht


Registered: 06/17/06
Posts: 9,270
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-Fahtty Mycelium syringe tek- 4
#5781218 - 06/22/06 05:45 PM (17 years, 7 months ago) |
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Edit: I've changed the title of this thread and taken out the word "isolation". It was disputed by a couple members and since the time of making this thread, have agreed that this is NOT an isolation technique. Isolation is done by multiple transfers on agar. I have since written up a tek for cloning (which is still not entirely guaranteed to be an isolate) that makes this tek less useful if you are desiring something closer to an isolate. Which is here: My oven bag clone tek With this being said, the one drop tek is still useful in the sense that it reduces the amount of colonies one is working with by only using one drop instead of multiple cc's of inoculate. It's still a great tek for the beginner and does have it's advantages. 
theres a slight change in this tek... i use the lids in this post now... super safe lids you'll see a HUUUUUUGE difference in the dropping of contam rates when you switch to silicone inoculation ports over tape... it's really the difference from a good grower to a great one.
heres how i prepare my corn...
First, put your corn in the pc without your jar rack (the plate the pc comes with to keep the jars off the bottom of the pc.) and cover with at least three inches of water. you can probably go a little less once you get the hang of how much the dry kernels will absorb. on average the kernel will expand 3 times it's size. Now pc at 15 psi for 1 hour. after an hour, turn off the pc and left the pressure adjust back to zero... once the safety nob drops, you can unscrew the lid. be very careful.. the pc will still be very hot. don't let the steam get ya in the face. Now pour the corn into a strainer. I rinse off the corn with hot water first. the kernels will have lots of starch on them.. if you rinse with cold water first the starch will coegulate making it a bitch to get off. rinsing is a very important part of the process... you want to get the kernels as free from starch as possible. once you've rinsed with hot water... rinse with cold. this will cool the kernels down making them easier to handle and also 'tighten' the kernel up making it not so mooshy. once rinsed, i let the corn sit for a few minutes to drain. Next i lay down a piece of paper towel and lay the corn on top of that in a single kernel layer. then i place another piece of paper towel on top of the corn layer and roll my hands lightly over the corn. this will wipe off excess water and starch from the corn before i place it in the 'faht jars'. next the new faht jars go into the pc again for another 45 mins. allow the pc to cool this time before opening. make sure you shake the jars once they are out of the pc to redistribute the starchy water that will indefinately end up poolin on the bottom of the jar and thats it.
heres the tek... -Fahtty Isolation/Mycelium syringe tek- 1. Overview - This is a tek that I use to isolate substrains and make mycelium syringes at the same time without the use of a glovebox or flowhood. Although it is required that you are experienced with grain substrates. This can be done in a few minutes while making you spawn grain jars/bags what have you. First take a clean 1/2 pint jar and fill the bottom with a 1/2 inch or so of hydrated grain substrate. (Note- I used dry popcorn kernels just for demonstration purposes only... you are going to use hydrated grain). Here are a couple examples..

Now cover with two layers of tyvek and lid band (this is what i use, you can use polyfil or any number of ways to seal the jar) and PC normal, 15 psi for 45 min...
When this is done and cool remove from PC and clean the center of the tyvek with rubbing alcohol....
Now poke thru the center of the tyvek with a spore syringe (sterile of course) and ONLY PUT ONE DROP IN THE MIDDLE... I have found that this is VERY hard to do.. lol but it is ESSENTIAL for this tek to work....
Now DO NOT SHAKE, DISTURB, OR EVEN MOVE the jar... if you can, inoculate the jar in the exact place you are going to let it colonize. The reason that you don't want the jar to be disturbed is that you want the spores to germinate as close together as you can to allow ONE dominate substrain to 'eat' the rest. Don't even look at the jar for five days. This how you get a substrain. Now you are going to let this substrain colonize the little bit of grain on the bottom of the 1/2 pint. Remember to tape up the tyvek 95% or your grain will dry out. When finished it should look something like this..
Now it's time to make the syringes you are going to use to innoc. your actual substrate. I usually make three to four syringes per 'faht jar'. I'll demonstrate how to make three. Take a clean, empty, 1/2 pint jar and put 25 ml. of water in it. This is the water that is going to fill up your three syringes that are going to innoc. your substrate. cover with two layers of tyvek, a band, and foil over the whole deal and set in pc. Now fill one syringe with distilled water and put in a 1/2 pint jar, needle first filled 3/4 of the way with distilled water. (basically you'll have a syringe sitting in a jar filled with water.) Now set this into the pc as well. what this does is keeps the syringe full of water.. I don't know about you, but opening a pc to find an empty syringe when you wanted to find a full one sucks. this happens cuz the pressure in the cooker forces the water out of the syringe... then the pc cools down and the pressure equals out, but if the needle is submerged in water, it will suck up the water instead of air. Now take your other two syringes and wrap them up in foil. place those in the pc also. pc at 15 psi for 30 min. Once the pc has cooled, remove the jar of water and three syringes (one full of water and the other two wrapped in foil) Now take your colonized 1/2 pint iso. and break it up in the jar by hitting the jar against your hand, It shouldn't take much...
Now clean off an injection spot on the tyvek with Rubbing alcohol...
Now take your one syringe full of water and flame the needle. then inject the water into the jar...
Now remove the needle and quickly place a piece of tape over the needle hole in the tyvek...
Now kind of gently shake the jar to get the mycelium to come off the kernels and into the water from your plain water syringe. This acts kind of like a organic eberbach container. :-) ...

Once completed, clean off another injection spot with R.A. and take your now empty plain water syringe and poke thru the tyvek (you are going to want to do this as close to the edge of the jar lid as possible)..

push the needle up against the side of the jar. tilt the jar so you get as much water by the syringe as you can and suck up the mycelium ladened water... when done your syringe should look something like this....
As you can see the syringe is now only half full. This is because some of the water 'stuck' to the corn kernels.. this is ok.. as you can also see, there is so much mycelium in the syringe that it has a blue tint to it and you can't see thru it. :-) Now this is where that jar of water and the other two syringes come in. take the syringe with the myc. laiden water in it and inject that into the jar with the water for the other two syringes. just squirt that first syringe in there and suck up a full syringe of the newly mixed water.. this is why you put in 25 ml.. 20 ml is for two syringes and the 5 ml. is for the other half of the myc. laiden syringe. clean off two more injection spots for the other two syringes and you have three fully stocked myc. syringes. be sure to cover any holes in the water jar until all syringes have been filled as not to contaminate your myc. water. this tek works great for multiple reasons... you can use it to get a substrain.. or to get mycelium syringes. you can also use this tek for cloning.. just drop in a piece of mushy from the inside of a stem that was dipped in h2o2 for a couple seconds. and there you go! (works best if done in a glovebox cuz the jar must be opened) you can also use this tek to store substrains.. mycelium can be dried and reanimated with water. just take off the tape on the tyvek (minus the one that is covering your innoc. point) that you use to keep the grain hydrated and let dry out.. thats it. I've had very little problems with this tek.
I've seen that Doc has a similar tek here with brf and water.. it's a nice tek. the only thing i see that this has up on that is that you have to remove the lid to get at the brf agar. you can also regrow the faht jars after you use them the first time just by taking off a bit of tape on the lid and letting them dry out for a couple days. then replace the tape and they should grow back fine. 
fahtster
Edited by fahtster (10/11/19 06:38 PM)
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Mezcal
Registered: 08/11/05
Posts: 1,980
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Re: -Fahtty Isolation/Mycelium syringe tek- [Re: fahtster]
#5781272 - 06/22/06 06:03 PM (17 years, 7 months ago) |
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Looks solid- well done!
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ackmess
Stranger
Registered: 06/09/06
Posts: 56
Last seen: 14 years, 7 months
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Re: -Fahtty Isolation/Mycelium syringe tek- [Re: Mezcal]
#5781284 - 06/22/06 06:07 PM (17 years, 7 months ago) |
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great tek.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: -Fahtty Mycelium syringe tek- [Re: fahtster]
#21282305 - 02/16/15 11:34 AM (8 years, 11 months ago) |
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Yup. Thought I should bump this since I started one.
Very nice Fahtster!!
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: -Fahtty Mycelium syringe tek- [Re: blackdust]
#21283112 - 02/16/15 02:08 PM (8 years, 11 months ago) |
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Cool, I was wondering how you did the drying. How long does dry mycelium last? Also, about how long is the recovery?
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: -Fahtty Mycelium syringe tek- [Re: fahtster]
#21283309 - 02/16/15 02:46 PM (8 years, 11 months ago) |
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The plastic syringe won't melt? Don't they come pre sterilized in a sealed package Anyways?
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: -Fahtty Mycelium syringe tek- [Re: Stipe-n Cap]
#21283346 - 02/16/15 02:52 PM (8 years, 11 months ago) |
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You can but autoclavable syringes, making them reusable.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: -Fahtty Mycelium syringe tek- [Re: Grey]
#21283373 - 02/16/15 02:58 PM (8 years, 11 months ago) |
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So the sterile packaged syringes are pc'able?
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: -Fahtty Mycelium syringe tek- [Re: Grey]
#21283379 - 02/16/15 02:59 PM (8 years, 11 months ago) |
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Quote:
ingclassy said: Cool, I was wondering how you did the drying. How long does dry mycelium last? Also, about how long is the recovery?[/quote
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: -Fahtty Mycelium syringe tek- [Re: d0urd3n]
#21283396 - 02/16/15 03:02 PM (8 years, 11 months ago) |
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i thought faht blessed the boards...but nice bump
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: -Fahtty Mycelium syringe tek- [Re: cronicr]
#21283453 - 02/16/15 03:14 PM (8 years, 11 months ago) |
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Oh damn, 8 years.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: -Fahtty Mycelium syringe tek- [Re: cronicr]
#21283459 - 02/16/15 03:15 PM (8 years, 11 months ago) |
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anyone reading take the date into consideration.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: -Fahtty Mycelium syringe tek- [Re: cronicr]
#21283468 - 02/16/15 03:16 PM (8 years, 11 months ago) |
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Blackdust bumped it,
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: -Fahtty Mycelium syringe tek- [Re: taGyo]
#21283474 - 02/16/15 03:17 PM (8 years, 11 months ago) |
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fahts still around so i have no issue with that
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: -Fahtty Mycelium syringe tek- [Re: d0urd3n]
#21283487 - 02/16/15 03:19 PM (8 years, 11 months ago) |
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Thank you d0urd3n, still curious how long dried mycelium can last.
Quote:
d0urd3n said:
Quote:
ingclassy said: Cool, I was wondering how you did the drying. How long does dry mycelium last? Also, about how long is the recovery?[/quote
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: -Fahtty Mycelium syringe tek- [Re: Grey]
#21283516 - 02/16/15 03:26 PM (8 years, 11 months ago) |
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Its basically just GLC its still seems relevant.
I am curious about the dried mycelium part though. I have heard people mention it but I would like to see a tek about it or hear more.
Edited by d0urd3n (02/16/15 03:26 PM)
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: -Fahtty Mycelium syringe tek- [Re: d0urd3n]
#21283649 - 02/16/15 03:55 PM (8 years, 11 months ago) |
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haha... I wish I could have seen my own look on my face when I saw this thread in my list lol. Yeah, as far as GLC goes, I'd direct you to the first link in my sig. I think some people still use the one drop thing on agar etc so I guess that's still slightly viable. Most will say to go to agar instead of GLC due to high bacteria in large jars of grain... which is probably a good idea, but being that the amount of grain per jar that I was PC'ing for the amount of time was so small, I was pretty damn confident that my masters were clean. I was also really confident in my lid design... that's the real key to drying the grains out... you have to be able to do it fast before the myc kicks into fruiting mode. I was more confident in my master jars that I was ever doing a G2G in a SAB. Now if I had a flow hood, THEN, yeah, I would have been doing agar for sure, but I didn't have one, so I improvised. Worked very well for me, but I'm weird so who knows. lol
The longest I had a dried jar that I used without problems was a little over a year and that was without adding water every few months... you could probably go longer if you rehydrate it and dry it back out again every-once-in-a-while to keep it from going bone dry.
faht
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: -Fahtty Mycelium syringe tek- [Re: fahtster]
#21283660 - 02/16/15 03:56 PM (8 years, 11 months ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: -Fahtty Mycelium syringe tek- [Re: cronicr]
#21283665 - 02/16/15 03:57 PM (8 years, 11 months ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: -Fahtty Mycelium syringe tek- [Re: fahtster]
#21283744 - 02/16/15 04:10 PM (8 years, 11 months ago) |
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Quote:
fahtster said: haha... I wish I could have seen my own look on my face when I saw this thread in my list lol. Yeah, as far as GLC goes, I'd direct you to the first link in my sig. I think some people still use the one drop thing on agar etc so I guess that's still slightly viable. Most will say to go to agar instead of GLC due to high bacteria in large jars of grain... which is probably a good idea, but being that the amount of grain per jar that I was PC'ing for the amount of time was so small, I was pretty damn confident that my masters were clean. I was also really confident in my lid design... that's the real key to drying the grains out... you have to be able to do it fast before the myc kicks into fruiting mode. I was more confident in my master jars that I was ever doing a G2G in a SAB. Now if I had a flow hood, THEN, yeah, I would have been doing agar for sure, but I didn't have one, so I improvised. Worked very well for me, but I'm weird so who knows. lol
The longest I had a dried jar that I used without problems was a little over a year and that was without adding water every few months... you could probably go longer if you rehydrate it and dry it back out again every-once-in-a-while to keep it from going bone dry.
faht
Yeah Dude, I plan on making clones this way. I have been playing with popcorn all week. I like the PC for hydrating the grain too.
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