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Amazon Shop: Agar, Brown Rice Flour, Laminar Flow Hood

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InvisibleSouthernGent
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Registered: 01/12/02
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a general question to all vendors
    #577623 - 03/13/02 06:47 AM (15 years, 4 months ago)

Have you guys checked out the KS syringes Josh is selling at the spore exchange? My question is this. Why is it you guys are selling clear syringes or ones with one clump in them when this guy can make syringes like that? I m not a spore vendor but if I was i d sell them that looked dark like Joshuas.


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OfflineAnnoA
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Re: a general question to all vendors [Re: SouthernGent]
    #577685 - 03/13/02 09:28 AM (15 years, 4 months ago)

I?m not a spore vendor anymore, but I think I can answer your queston.

It doesn?t matter if a syringe is purple or not.
If there are 100 000 spores in it(clear) then the syringe is equally good as whern there are 1 000 000(purple).

What matters much more is that the the syringe is contaminant free, fresh and that the strain has been tested and prooved to work good both as cakes and casings.

That?s what counts, not the color of the suspension.


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InvisibleSouthernGent
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Re: a general question to all vendors [Re: Anno]
    #577707 - 03/13/02 10:08 AM (15 years, 4 months ago)

Thanks Anno.


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InvisibleJoshua
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Re: a general question to all vendors [Re: Anno]
    #577860 - 03/13/02 02:08 PM (15 years, 4 months ago)

I agree with you regarding the sterility of a syringe. By ordering from a vendor your chances of getting a sterile syringe is much higher. I also agree w/ the other vendor advantages.

I prepared those syringes via the oven tek. I don't have access to expensive equipment such as a flow hood. The fruits were grown out from a casing of BRF cake substrate.

On the other hand I do believe that a higher density spore syringe has its advantages. I have seen cakes inoculated w/ heavy syringes go much faster than cakes w/ lighter ones. Also, to a certain degree, a heavy syringe has more genetic variability and the possibility of fuiting a good strain should be a bit higher.

I will do an experiment soon. I will inoculate a substrate w/ equal volumes of spore water from a light syringe and a heavy syringe. We will see if there are differences.

Joshua


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Offlinejonnyshaggs420
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Re: a general question to all vendors [Re: Joshua]
    #577941 - 03/13/02 04:34 PM (15 years, 4 months ago)

I still think its because vendors can make more money off of dilute syringes then dark ones. If they did sell dark syringes I personally would just dilute them upon arrival to spread them out more, and get more use out of them....using a dark syringe straight is just a waste of spores.


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Offlinekarlfinn
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Re: a general question to all vendors [Re: Joshua]
    #577975 - 03/13/02 05:20 PM (15 years, 4 months ago)

do you work any with h2o2 since no hood....??
found that working in a bag, wiped with straight h2o2
with just sterile loop to get spores to water for syng. fills.
I keep a damp h2o2 paper towel over my other tools.
so far so good...thanx for the heads up I will take some of your
liquid to the agars and watch and select...let you know haw the cantam count is.
also working with liquid teks. dextrose and malts...esp. for isolated myc. strains...
I check areas out with exposed agar plates for one minute...especially to see if my old hood is still working well enough....
keep up the great work and service to all.


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Offlinepleezr
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Re: a general question to all vendors [Re: jonnyshaggs420]
    #578040 - 03/13/02 07:13 PM (15 years, 4 months ago)

jonny, i dont think diluting a dark syringe is a very good idea. to risky


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InvisibleJoshua
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Re: a general question to all vendors [Re: karlfinn]
    #578223 - 03/13/02 11:14 PM (15 years, 4 months ago)

I use isopropyl alcohol to wipe down surfaces and tools. I pressure cook used syringes and the water that I use. I have had no contams using this proceedure. All of the contams I get (if you have seen some in my posts:sad:) are from the casings. I will do an agar viability test soon. I am out of pre-prepared plates right now. I will post the results in my original post.

Joshua 


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Edited by Joshua (03/14/02 05:15 AM)


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Amazon Shop: Agar, Brown Rice Flour, Laminar Flow Hood

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