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OfflineLilDon697
street chemist
Registered: 02/03/02
Posts: 267
Loc: Jerzy
Last seen: 9 years, 9 months
Heres my plan!!!! Any help will be appreciated!
    #575926 - 03/11/02 02:18 PM (22 years, 23 days ago)

Ok . .i have tried once and failed with all 12 jars . . . .little green monster!!!

I think it had alot to do with my sterility!
Heres my plan!

Day one- I am goin to buy organin brown rice and use a coffe grinder at the store to grind it. Then I will prepare 12 jars maybe more of brf and verm. From there i will cap like normal (tighten lids and put tape over holes and cover with aluminum) then i will have a low boil for about an hour! Then let everything kool.

Day two- I am goin to take my sterilezed and place them in a room . .spray some of my 50/50 bleach n water in the room followed by some lysol.

I can get syringes in any store around me . . i wanna order them but should my mom open my mail for the first time it would look really odd! I am goin to have to stick with prints.

I will take a needle (sewing needle) Sterilize it with alcohol and then flame it. Scrape of some of my print and close the cap . .and then put my filter over it!

Then i will place in a rubbermaid container on top of a heating pad!

How does this sound?


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A Pimps only as good as his hoes!!!

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OfflineBeppoMarx
old hand
Registered: 09/01/01
Posts: 1,126
Last seen: 19 years, 4 days
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #575933 - 03/11/02 02:22 PM (22 years, 23 days ago)

sounds perfect. just dont let anything other than psilocybe mushroom spores in that jar when you have it open and youll be fine.


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Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
BUCKETS BRIGADE left hand man!!!

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OfflineLilDon697
street chemist
Registered: 02/03/02
Posts: 267
Loc: Jerzy
Last seen: 9 years, 9 months
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #576147 - 03/11/02 06:57 PM (22 years, 23 days ago)

well i ground my br before and 2morrow i will prepare my substrate. . . .
i will like i said boil for one hour!!!
How much water and verm and br should i use (im doing 12 jars) . .how many cups of each considering im boiling and can i put honey in the water?

I will let my substrate sit for a day and if i see no contams i will proceed to innoculate my jars! Im going to use a sewing needle to scrape the print off!

I hope this works!!!!!!!!!


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A Pimps only as good as his hoes!!!

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Offlinefresh357
addict

Registered: 09/02/01
Posts: 493
Last seen: 21 years, 11 months
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #576150 - 03/11/02 07:02 PM (22 years, 23 days ago)

innoculate over the oven.

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OfflineLSD_4me
addict
Registered: 02/09/02
Posts: 416
Last seen: 21 years, 10 months
Re: Heres my plan!!!! Any help will be appreciated! [Re: fresh357]
    #576227 - 03/11/02 08:50 PM (22 years, 23 days ago)

you can get syringes search for feed stores, there like 75 cents a piece man...

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Offline2shrewmen
deep seadwellers

Registered: 03/03/02
Posts: 77
Loc: Bikini Bottom
Last seen: 16 years, 2 months
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #576543 - 03/12/02 03:50 AM (22 years, 22 days ago)

According to MMGG, the recipe is 2/3 c. vermiculite, 1/4 c. brf, 1/4 c. water. If you grind your own flour, which we did, add 1&1/2 tablespoons water. To further protect from contams, spread verm & brf on cookie sheets and bake for 20 mins. at 300 F. We did this and have had success so far (Just started birthing cakes 2 days ago). Also, why not start slow and learn? We ordered two syringes, but only inoculated six jars. That way if we f up, we have more to try again. Unless you have unlimited resources of course.shrewman1

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OfflineAnnoA
Experimenter
 User Gallery

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Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 12 hours
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #576549 - 03/12/02 04:15 AM (22 years, 22 days ago)


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Offlinefrogsheath
Stranger
Registered: 02/18/02
Posts: 915
Loc: Chicago, Illinois U.S.A.
Last seen: 8 years, 9 months
Re: Heres my plan!!!! Any help will be appreciated [Re: LilDon697]
    #576554 - 03/12/02 04:34 AM (22 years, 22 days ago)

LilDon,
You don't want to tighten the lids BEFORE sterilizing.
According to the PF tek you leave 'em loose while sterilizing
so the steam permeates the substrate, then tighten AFTER
they cool down you tighten. Correct me if I'm wrong but that's what
I did. I'm a newbie too.

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Invisiblemrdasani
enthusiast
Registered: 10/19/01
Posts: 224
Re: Heres my plan!!!! Any help will be appreciated [Re: frogsheath]
    #576558 - 03/12/02 04:39 AM (22 years, 22 days ago)

You dont need to tighten the lids after. Its okay to leave them loose.


--------------------
"Come on you raver, you seer of visions,
come on you painter, you piper, you prisoner, and shine!" - Pink Floyd (Wish You Were Here 1975)

"Never underestimate the power of denial." -from American Beauty.

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Heres my plan!!!! Any help will be appreciated [Re: mrdasani]
    #576586 - 03/12/02 06:25 AM (22 years, 22 days ago)

From the PF Tek, Chapter 1:"After the jars have cooled,
tighten the lids and store them in a cool draft free place until
ready to inoculate them"( www.fanaticus.com ):peace:


Free Spore Ring

 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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Invisiblepsyphon
mneumatic device

Folding@home Statistics
Registered: 11/27/01
Posts: 565
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #576644 - 03/12/02 08:26 AM (22 years, 22 days ago)

If you can get a pressure cooker that will greatly help with sterility.  If you have to boil, do it at higher temps with a tight fitting lid.  If you can use a syringe do so, its much easier, then you can just innoculate through the holes in the lids.  And to be even more sterile, bring your oven up to about 225 - 250deg, open the door and innoculate on the oven door, the hot air pouring out should be nice and clean and keep your innoculations clean.  Also you can wipe your needle between jars with rubbing alcohol.  As for the lid tightness, I'd say leave them loose during sterilization with tape over the holes, tighten as soon as you take them out of the water, innoculate by pulling back the tape and replacing and then loosen the lids as the jars start to grow.

Hope this helps :smile: 


--------------------
"The real voyage of discovery consists not in seeking new landscapes but in having new eyes."
- Marcel Proust

I wish you all ceaselessly flowing moments of happiness.

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OfflineLilDon697
street chemist
Registered: 02/03/02
Posts: 267
Loc: Jerzy
Last seen: 9 years, 9 months
Re: Heres my plan!!!! Any help will be appreciated! [Re: psyphon]
    #576681 - 03/12/02 09:13 AM (22 years, 22 days ago)

thx . .i was only gunna tighten the lids cause i have the two piece lids but w/e . .ill leave em loose and asnd then tighten.

thx for all your help . .im about to start makin my substrate now . .thx again!


--------------------
A Pimps only as good as his hoes!!!

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Offlinezodiac312
journeyman

Registered: 02/11/02
Posts: 58
Last seen: 21 years, 8 months
Re: Heres my plan!!!! Any help will be appreciated [Re: WakingUpLate]
    #576765 - 03/12/02 10:41 AM (22 years, 22 days ago)

i read on one of the beginner teks to leave the jars tight but leave the tape off of the nail holes. it said that steam getting into the jars would screw up the water ratio. why would you bother to use foil to cover the lids if you wanted water\steam to get into your jars? could somebody please clarify this for me? a friend of mine did some jars with the lids tight and the tape holes uncovered, only with foil crumpled over the top. thanks in advance.


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*Everything in this post is fictional and should be treated as such*

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Heres my plan!!!! Any help will be appreciated [Re: zodiac312]
    #576896 - 03/12/02 01:01 PM (22 years, 22 days ago)

From PF Tek, Chapter 1 :"When steaming or pressure canning is performed, the jars must be protected from water dripping down from the underside of the pot lid caused by heavy condensation and drip off during boiling. This water can get into the jars by entering under the jar lids that aren?t tight and soaking the substrate - throwing off the formula and setting up failure. To prevent this, wrap some tin foil around the cap to ward off the water. The tin foil can be removed after steaming (with the tape guarding the needle holes - or the tin foil can be left on until it is inoculation time."  ( www.fanaticus.com/sub-prep.htm ):peace:


Free Spore Ring

 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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OfflineLilDon697
street chemist
Registered: 02/03/02
Posts: 267
Loc: Jerzy
Last seen: 9 years, 9 months
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #576986 - 03/12/02 02:22 PM (22 years, 22 days ago)

well i just sterilized my jars . .i had a question tho . .i had left the lids loose and put tin foil around the jar . .i took my jars out of the pot . .it likes like the brown rice was kinda cooked . . .(its fluffy) . .its that ok? . .its not everywhere like thatr but some i guess didnt get ground up well enough and ghot fluffy . . .thats alright . . .right?

also for my incubator i have a rubbermaid container . .i put a heating pad on the bottom and a towel over it . . . .i plan to just put the filters on my jars and then place them in it . . . will that be alright?


--------------------
A Pimps only as good as his hoes!!!

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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #577023 - 03/12/02 03:06 PM (22 years, 22 days ago)

Dude, unless I'm reading this wrong, you've already steralized it, and haven't put the filters on yet?
I hope, for your sake, that's not the case.:peace:



Free Spore Ring

 


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

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OfflineLilDon697
street chemist
Registered: 02/03/02
Posts: 267
Loc: Jerzy
Last seen: 9 years, 9 months
Re: Heres my plan!!!! Any help will be appreciated! [Re: WakingUpLate]
    #577105 - 03/12/02 04:38 PM (22 years, 22 days ago)

no u read it right . .i was innoculating from a spore print . . .i had tin foil over the jars as i sterilized. I then proceeded to innoc the jars and then put a coffee filter over each jars . . .isnt that right? (not to mention i put the lids onm before the filter and have two holesin lid for air exchange!


--------------------
A Pimps only as good as his hoes!!!

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OfflineLilDon697
street chemist
Registered: 02/03/02
Posts: 267
Loc: Jerzy
Last seen: 9 years, 9 months
Re: Heres my plan!!!! Any help will be appreciated! [Re: LilDon697]
    #577111 - 03/12/02 04:43 PM (22 years, 22 days ago)

oh and they are siting in my incubator right now which isnt very fancy . . .just a rubbermaid container with (green) with a heating pad on the bottom and small towel over the heating pad on which my jars are resting. The lid is on also . .how much is good tho? Should i have the heaitng pad on high medium or low? . . . it isnt a very strong heating pad and the towel absorbs alot of the heat too!


--------------------
A Pimps only as good as his hoes!!!

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Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   MagicBag.co Certified Organic All-In-One Grow Bags   Bridgetown Botanicals CBD Concentrates


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