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Offlinelocalground
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Registered: 05/16/06
Posts: 46
Last seen: 15 years, 2 months
should this work?
    #5764854 - 06/18/06 04:04 PM (17 years, 7 months ago)

ijust got some pre sterilized jars and inoculated them , i kept everything pretty sterile with alcohol, i rubbed the tops of the jars down with 91% alcohol and the needle tip. Should the holes at the top have been sealed after inoculation , i hear some people leave them open for air to come in. Do you think i'll get mycellium with the tops open? I used the brazilian strain by the way. I inoculated them yesterday and they are sitting in a cardboard box that i rubbed alcohol on the inside of. AS you might be able to tell ive never done this before.. let me know what you think. And how long should it take for mycellium to show up?


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OfflineSnaggletooth
Stranger in a Strange Land
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Registered: 10/24/05
Posts: 6,109
Loc: blinks stupidly
Last seen: 6 years, 8 months
Re: should this work? [Re: localground]
    #5764872 - 06/18/06 04:08 PM (17 years, 7 months ago)

if your doing the PF tek, there should be a dry layer of vermiculite on top, then you dont need to cover them up.


Never don PF but I think I might, but I would use this tape,


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Invisibledysphoria
lost soul

Folding@home Statistics
Registered: 06/04/03
Posts: 1,651
Re: should this work? [Re: localground]
    #5764886 - 06/18/06 04:11 PM (17 years, 7 months ago)

should take anywhere from 3 to 10 days
but do NOT post anything until about day 14-18 of no growth
sometimes, its just too early to tell..even if its really late for you
all things considered, sounds like you did ok
im sure you'll get some growth in a couple jars, probably not all

one of the most preferred methods is to cover the holes with micropore tape, aka surgical tape, more specifically the 'gentle paper' surgical tape, nexcare or comparable brand. i suppose cloth bandaids would work too. just dont do plastic...its solid plastic, with small holes punched in it every 1mm or so, which coincidentally, can mean your small hole is covered entirely by non-permeable plastic.

other than that, good job, and dont worry, before you know it...you'll see signs of growth, and then you're hooked.


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Invisibledysphoria
lost soul

Folding@home Statistics
Registered: 06/04/03
Posts: 1,651
Re: should this work? [Re: dysphoria]
    #5764895 - 06/18/06 04:13 PM (17 years, 7 months ago)

...and before i can even finish my post, theres snaggle, posting a picture of exactly what i describe =)


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