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OfflineLeimana
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Pan cyans fruiting invitro?!
    #5761757 - 06/17/06 08:40 PM (17 years, 7 months ago)

I made up some Pan cyan spawn and spawned it to about 2 gallons of horse poo/newspaper/cardboard mix. After about 5 days, it looked colonized, but circumstances required that I let it go for a while. It has been about 15 days since I spawned to the bulk substrate and put it into a spawn bag to colonize. I looked at it this morning, and I have mushrooms growing in the bag.

I did not think this would happen because my understanding of Pan cyans was that they needed lots of FAE, and would not fruit without a casing layer, neither of which they have had.

At this point, the fruits look a lot like Ps. cubensis, with longer than usual stems and small caps, all of which could be explained by the build up of CO2 in the bag, but the syringe that I used to create the spawn has twice in the past produced mushrooms that were unmistakeably Pan cyans. Needless to say, I am more than a little suprised and confused.

Any one ever seen this before? Can I open the bag, pick off the fruitbodies and primordia and crumble and case as I had planned?

Thanks,

Leimana


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OfflineSnaggletooth
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Re: Pan cyans fruiting invitro?! [Re: Leimana]
    #5761764 - 06/17/06 08:42 PM (17 years, 7 months ago)

Wheres the Pic!!!!


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Offlinecoda
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Re: Pan cyans fruiting invitro?! [Re: Snaggletooth]
    #5762352 - 06/17/06 11:17 PM (17 years, 7 months ago)

yah, pic is definitely needed in this situation :smile:

It's happened before, there is a slight chance you have a mislabeled syringe.


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

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i really am glad you came back to us instead of taking the other path. *hug*

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Don't fuck with the laughing jesus.


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OfflineLeimana
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Re: Pan cyans fruiting invitro?! [Re: coda]
    #5763109 - 06/18/06 02:56 AM (17 years, 7 months ago)

Lights! Camera! Pictures:

The bag:


The syringe:


I got the syringe from Ralph, and, as I mentioned earlier, this same syringe HAS produced mushrooms that were unmistakeably Pan cyans.

Thanks,
Leimana


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Offlineiamyour_messiah
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Re: Pan cyans fruiting invitro?! [Re: Leimana]
    #5763640 - 06/18/06 08:41 AM (17 years, 7 months ago)

Hmm I dunno too much about this, but I was sure Pan. cyanescens was a white stemmed white capped mushroom, possibly faintly yellow capped. The caps in that pic seem pretty brown so are you sure its really Pan. cyan? It is of couurse possible that the caps fade to a lighter color with time, something which I wouldnt known as I've never grown them before...

EDIT: sry, after posting I read the last thing you wrote about the syringe having produced what you are sure were Pan. cyan in the past. Nvm in that case. Also, I must admit that I never even read the original post to begin with. I just scrolled to the pics and posted. My apologies.


Edited by iamyour_messiah (06/18/06 08:45 AM)


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OfflineMLBjammer
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Re: Pan cyans fruiting invitro?! [Re: iamyour_messiah]
    #5763981 - 06/18/06 10:44 AM (17 years, 7 months ago)

From that pic, they don't look much like Pans to me, either, but a lot like Cubensis. Maybe some Pan growers will take a look at this thread.


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OfflineEquilibriuM
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Re: Pan cyans fruiting invitro?! [Re: MLBjammer]
    #5763993 - 06/18/06 10:50 AM (17 years, 7 months ago)

strange


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InvisibleUnderNose
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Re: Pan cyans fruiting invitro?! [Re: EquilibriuM]
    #5764004 - 06/18/06 10:54 AM (17 years, 7 months ago)

They look Cubie to me to


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Offlinecoda
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Re: Pan cyans fruiting invitro?! [Re: UnderNose]
    #5764193 - 06/18/06 12:26 PM (17 years, 7 months ago)

those look like cubensis to me. If you take a look at the largber pin in the first photo i see what appears to be a veil. Cyan's don't have veils.


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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Offlinejohnjohnandjamal
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Re: Pan cyans fruiting invitro?! [Re: coda]
    #5764240 - 06/18/06 12:44 PM (17 years, 7 months ago)

Do you also grow cubes? Maybe you got a cubensis contamination.


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OfflineWorkmanV
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Re: Pan cyans fruiting invitro?! [Re: johnjohnandjamal]
    #5764503 - 06/18/06 02:15 PM (17 years, 7 months ago)

I agree, cubensis contamination. It happens all the time. It is almost as if pan cyan colonized manure is a selective media for cubensis.


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InvisiblePsychoslut
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Re: Pan cyans fruiting invitro?! [Re: Workman]
    #5764658 - 06/18/06 02:49 PM (17 years, 7 months ago)

A guy couldnt ask for a better contam  :smirk:.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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OfflineLeimana
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Loc: By the Tum-Tum tree, in u...
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Re: Pan cyans fruiting invitro?! [Re: Workman]
    #5765998 - 06/18/06 11:17 PM (17 years, 7 months ago)

Quote:

Workman (!) said:
I agree, cubensis contamination. It happens all the time. It is almost as if pan cyan colonized manure is a selective media for cubensis.




Shit! That's possible? Time to make a either a flowhood, or a glove box big enough to work with 2-3 gallons of substrate.


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OfflineLeimana
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Re: Pan cyans fruiting invitro?! [Re: Psychoslut]
    #5766024 - 06/18/06 11:22 PM (17 years, 7 months ago)

Quote:

Psychoslut said:
A guy couldnt ask for a better contam  :smirk:.




Yeah, ok, it IS better than my usual bumper crop of trich.


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InvisibleSatisfy
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Registered: 08/24/09
Posts: 231
Re: Pan cyans fruiting invitro?! [Re: Leimana]
    #10960953 - 08/29/09 12:04 PM (14 years, 5 months ago)

Quote:

Leimana said:
Quote:

Psychoslut said:
A guy couldnt ask for a better contam  :smirk:.




Yeah, ok, it IS better than my usual bumper crop of trich.




Bahahah.  ^^


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Invisiblecyb3rtr0n
searching for truth
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Registered: 05/06/09
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Re: Pan cyans fruiting invitro?! [Re: Satisfy]
    #10960966 - 08/29/09 12:11 PM (14 years, 5 months ago)

I've heard of delayed reactions to a joke which you have to think about.

but never a 3 year delayed reaction.


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OfflineThyrax
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Registered: 01/03/08
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Re: Pan cyans fruiting invitro?! [Re: cyb3rtr0n]
    #10960989 - 08/29/09 12:13 PM (14 years, 5 months ago)

what can you do to prevent cubensis spore to contam pan myc ?


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