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OfflineDal33tdude12
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Registered: 05/22/06
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Last seen: 17 years, 2 months
Sterilization Question!
    #5758900 - 06/16/06 09:54 PM (17 years, 7 months ago)

K so i have had about 4 or 5 jars contaminated and they ALL look like they were contaminated at the 4 holes in the lid where i innoc. All 4 points in all jars were the contaminated starting point. Im wondering if maybe me using my lil snap on butane torch wasnt cutting the sterilization? I figured it would work. I re torched the needle until it was glowing red after 3 jars, in a room where i sprayed bleach and lysol spray. From now on im going to make a glove box but i am wondering exactly what went wrong? Thanks all!


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Offlinefaceyneck
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Re: Sterilization Question! [Re: Dal33tdude12]
    #5758916 - 06/16/06 10:01 PM (17 years, 7 months ago)

Do you think the spore solution might be contaminated? Did you get it from a reliable source? How old is the solution?


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Invisibleniteowl
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Re: Sterilization Question! [Re: faceyneck]
    #5759678 - 06/17/06 02:48 AM (17 years, 7 months ago)

There are MANY reasons for contams.
A bad syringe is the first thing I would suspect.


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Offlinekilroy
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Re: Sterilization Question! [Re: niteowl]
    #5759706 - 06/17/06 03:19 AM (17 years, 7 months ago)

It does seem like a syringe if they all started at the innoc points. I would sugest triing an LC and see if that gets contam too or skip that part and get a hold of the vender to get a replacement. If you got it from a shroomery vender I am sure they will replace it. Even the best venders can send a contam syringe, no one is perfect and you know the old saying of shit happens. GL with your next one


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OfflineDal33tdude12
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Registered: 05/22/06
Posts: 52
Last seen: 17 years, 2 months
Re: Sterilization Question! [Re: kilroy]
    #5760453 - 06/17/06 12:06 PM (17 years, 7 months ago)

i got them from http://www.thehawkseye.com/ Are they reliable? Im not too sure that it is the syringe, well it could possible be i did 12 jars with no holes in the lids just opened them in a clean room and innoc, and 12 where i did have 4 holes and i didnt have any of the ones that had no holes contaminated. only the ones with holes in the lid.


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