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shroomseng
Stranger

Registered: 01/20/06
Posts: 201
Last seen: 7 years, 6 months
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400 Dollars and a Few Questions
#5755485 - 06/15/06 10:08 PM (17 years, 7 months ago) |
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Appx How long will complete colonization of a spawn bag take for Cubensis if I inject with only 10cc? The guide on this site recommends 140, but I believe spores come in 10... I don't remember but I know it was nowhere near that. Any advice on this is appreciated!
What should I invest in?
I already have the following:
Pressure Cooker Heating Cables (Is this an advisable way to maintain temperatures? Humidifier
I am planning to purchase:
A thermometer/ humidity meter Perhaps a glovebox??? Or glove bags. Are these recommendable? Or necessary or even helpful for spawn bag???
Or I can set up a room into being my sterile room.. where can I obtain er.. Big plastic sheets...? Does anyone have any experience on how much to spend or what to use for this? Assuming I'm going to take over a kitchen or bathroom or walk in closet.
After my bags are done I am just going to slice them open, and case, maybe use one to attempt en uber bulk.
Well ya.. The fun part of this thread now
Advise me what to spend my money on gear wise
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HippieChick
Chicks can do it too!


Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 13 days
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Re: 400 Dollars and a Few Questions [Re: shroomseng]
#5755493 - 06/15/06 10:11 PM (17 years, 7 months ago) |
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Invest $40 or so in setting up a 4 tier green house 
Make a LC from your syringe.
Peace,Love,Happiness and Harmony Hippie Chick
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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EquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
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Re: 400 Dollars and a Few Questions [Re: HippieChick]
#5755500 - 06/15/06 10:13 PM (17 years, 7 months ago) |
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yes, 140CC is supposed to be liquid culture. You can easily make 400CC Liquid Culture with 1 CC spore solution
-------------------- HELP!!!!!!!!!
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shroomseng
Stranger

Registered: 01/20/06
Posts: 201
Last seen: 7 years, 6 months
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Re: 400 Dollars and a Few Questions [Re: EquilibriuM]
#5755502 - 06/15/06 10:15 PM (17 years, 7 months ago) |
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Alright I will do that And that
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: 400 Dollars and a Few Questions [Re: shroomseng]
#5755804 - 06/15/06 11:48 PM (17 years, 7 months ago) |
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There are several sizes of spawn bags, but none that require more than ten cc to inoculate. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: 400 Dollars and a Few Questions [Re: shroomseng]
#5755877 - 06/16/06 12:25 AM (17 years, 7 months ago) |
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Quote:
shroomseng said: Appx How long will complete colonization of a spawn bag take for Cubensis if I inject with only 10cc?
I inoculate a 2kg bag with 1-2cc of spore solution, it's just not neccissary to use any more than that, the same follows with liquid cultures
Quote:
I am planning to purchase: Perhaps a glovebox??? Or glove bags.
building a glove box or flow hood is a good idea, they are some of the more useful tools in a lab, if doing bags they arent required at this point so you may want to look through the FAQ and see what other techniques are available before you spend all the cash
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EquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
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Re: 400 Dollars and a Few Questions [Re: Prisoner#1]
#5757269 - 06/16/06 01:11 PM (17 years, 7 months ago) |
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Quote:
Prisoner#1 said:
I inoculate a 2kg bag with 1-2cc of spore solution, it's just not neccissary to use any more than that, the same follows with liquid cultures
Not true. 2CC LC to spawn bag would take forever. while 140CC takes about 7 days.
-------------------- HELP!!!!!!!!!
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