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Offlineshroomseng
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Registered: 01/20/06
Posts: 201
Last seen: 7 years, 6 months
400 Dollars and a Few Questions
    #5755485 - 06/15/06 10:08 PM (17 years, 7 months ago)

Appx How long will complete colonization of a spawn bag take for Cubensis if I inject with only 10cc? The guide on this site recommends 140, but I believe spores come in 10... I don't remember but I know it was nowhere near that. :blush: Any advice on this is appreciated!

What should I invest in?


I already have the following:


Pressure Cooker
Heating Cables (Is this an advisable way to maintain temperatures?
Humidifier

I am planning to purchase:

A thermometer/ humidity meter
Perhaps a glovebox??? Or glove bags. Are these recommendable? Or necessary or even helpful for spawn bag???

Or I can set up a room into being my sterile room.. where can I obtain  er.. Big plastic sheets...? Does anyone have any experience on how much to spend or what to use for this? Assuming I'm going to take over a kitchen or bathroom or walk in closet.

After my bags are done I am just going to slice them open, and case, maybe use one to attempt en uber bulk.

Well ya.. The fun part of this thread now

Advise me what to spend my money on gear wise :smile:


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
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Re: 400 Dollars and a Few Questions [Re: shroomseng]
    #5755493 - 06/15/06 10:11 PM (17 years, 7 months ago)

Invest $40 or so in setting up a 4 tier green house :thumbup:

Make a LC from your syringe.

Peace,Love,Happiness and Harmony
:heart: Hippie Chick  :mushroom2:


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OfflineEquilibriuM
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Registered: 07/17/05
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Re: 400 Dollars and a Few Questions [Re: HippieChick]
    #5755500 - 06/15/06 10:13 PM (17 years, 7 months ago)

yes, 140CC is supposed to be liquid culture. You can easily make 400CC Liquid Culture with 1 CC spore solution


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Offlineshroomseng
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Re: 400 Dollars and a Few Questions [Re: EquilibriuM]
    #5755502 - 06/15/06 10:15 PM (17 years, 7 months ago)

Alright I will do that :smile: And that :smile:


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OfflineRogerRabbitM
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Re: 400 Dollars and a Few Questions [Re: shroomseng]
    #5755804 - 06/15/06 11:48 PM (17 years, 7 months ago)

There are several sizes of spawn bags, but none that require more than ten cc to inoculate.
RR


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InvisiblePrisoner#1
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Registered: 01/22/03
Posts: 193,665
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Re: 400 Dollars and a Few Questions [Re: shroomseng]
    #5755877 - 06/16/06 12:25 AM (17 years, 7 months ago)

Quote:

shroomseng said:
Appx How long will complete colonization of a spawn bag take for Cubensis if I inject with only 10cc?




I inoculate a 2kg bag with 1-2cc of spore solution, it's just not neccissary to
use any more than that, the same follows with liquid cultures



Quote:

I am planning to purchase:
Perhaps a glovebox??? Or glove bags.




building a glove box or flow hood is a good idea, they are some of the more
useful tools in a lab, if doing bags they arent required at this point so you
may want to look through the FAQ and see what other techniques are available
before you spend all the cash


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OfflineEquilibriuM
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Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
Re: 400 Dollars and a Few Questions [Re: Prisoner#1]
    #5757269 - 06/16/06 01:11 PM (17 years, 7 months ago)

Quote:

Prisoner#1 said:

I inoculate a 2kg bag with 1-2cc of spore solution, it's just not neccissary to
use any more than that, the same follows with liquid cultures






Not true. 2CC LC to spawn bag would take forever. while 140CC takes about 7 days.  :thumbup:


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