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Offlinephlegmbae
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How do I resterilize syringes for reuse?
    #5752923 - 06/15/06 09:49 AM (17 years, 7 months ago)

I have scoured the Shroomery for an answer for the past 2 hours on this subject to no avail! So could someone tell me how I can safely PC my used syringes for reuse? I used to have the old PF pamphlet that explained it in detail, but I have since sent that old information to a friend on the other side of the US, whom I've lost contact with. I want to primarily use the old syringes to inoculate an LC, and reuse them for making more spore syringes. Any information would be greatly appreciated!  :mushroom2:


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I'm a hole in time, in a time that's full of holes


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InvisibleAtheist
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Registered: 01/24/06
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Re: How do I resterilize syringes for reuse? [Re: phlegmbae]
    #5752936 - 06/15/06 09:53 AM (17 years, 7 months ago)

how did you not find anything?

look at the bottom of this page

and learn the search engine


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OfflineUncleMike
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Re: How do I resterilize syringes for reuse? [Re: phlegmbae]
    #5752945 - 06/15/06 09:56 AM (17 years, 7 months ago)

1. remove the plunger

2. place in pressure cooker

3. put water in pressure cooker

4. place lid on pressure cooker and cook for 15 min. @ 15 psi

that should do it. :wink:

p.s. don't forget to wrap in tin foil to keep the syringe off the bottom of the cooker


Edited by UncleMike (06/15/06 10:01 AM)


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OfflineDragonChaser
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Re: How do I resterilize syringes for reuse? [Re: UncleMike]
    #5753278 - 06/15/06 11:33 AM (17 years, 7 months ago)

Well when do you put the plunger back in Mike?
Dragon


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InvisibleRoadkillM
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Re: How do I resterilize syringes for reuse? [Re: phlegmbae]
    #5753541 - 06/15/06 01:09 PM (17 years, 7 months ago)

Don't listen to Uncle mike he is stoned!~

:smirk:

You would wash out the syringe...

then fill the syringe with water...

wrap in alum foil and pressure cook!~

Leaving the plunger in the syringe!~

---

You can either pressure cook them or follow this method....

1. Fill a cooking pot halfway with tap or distilled water (use distilled water if your tap water contains higher levels of minerals and chemicals).

2. Boil the water in the pot on high for a minimum of ten minutes, this should be adequate to sterilize and cleans the water of all bacteria, viruses, and mold spores.

3. Take your empty syringe and fill it with the boiling water. Allow it to sit for two minutes with the hot water inside. (If your syringe was sterility packaged, skip steps 4 and 5).

4. Purge the hot water from the syringe into a sink, not back into the saucepan.

5. Repeat steps 3 and 4 two or three more times. the last time leave the hot water in the syringe.

6. Place the syringe in a cool draft-free place, preferable in a clean zip-lock bag

7. Allow it to cool for several hours before using.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinecrakkattak
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Re: How do I resterilize syringes for reuse? [Re: Roadkill]
    #5753549 - 06/15/06 01:12 PM (17 years, 7 months ago)

Damn and all this time I thought syringes could not be home sterilized


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J-to tha R-O-C Knomsayn!


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OfflineOatman2000
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Re: How do I resterilize syringes for reuse? [Re: crakkattak]
    #5753766 - 06/15/06 02:15 PM (17 years, 7 months ago)

oh yes!... this works great too...

i have also found out that usually only B-D syringes can be pc'd. They can withstand up to the temps.


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:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
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OfflineINFECTEDGOAT420
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Re: How do I resterilize syringes for reuse? [Re: Oatman2000]
    #5753781 - 06/15/06 02:20 PM (17 years, 7 months ago)

listen to road kill


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InvisibleRoadkillM
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Re: How do I resterilize syringes for reuse? [Re: Oatman2000]
    #5753786 - 06/15/06 02:22 PM (17 years, 7 months ago)

Oatman brings up a good point...

not all brands of syringes are autoclaveable.

so test one of your syringes in the pressure cooker...
before melting down a bunch of them by making a mistake!~

:smile:



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinephlegmbae
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Re: How do I resterilize syringes for reuse? [Re: Roadkill]
    #5767977 - 06/19/06 01:11 PM (17 years, 7 months ago)

Thanks for the tips guys! Now I can pressurize LC jars safely!


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I'm a hole in time, in a time that's full of holes


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OfflineRogerRabbitM
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Re: How do I resterilize syringes for reuse? [Re: phlegmbae]
    #5768650 - 06/19/06 04:16 PM (17 years, 7 months ago)

I also have always just boiled syringes. There's no need to PC anything that doesn't harbor endospores, which plastic does not.
RR


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Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisibledurban_poison
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Registered: 09/19/01
Posts: 2,417
Re: How do I resterilize syringes for reuse? [Re: phlegmbae]
    #5768842 - 06/19/06 05:02 PM (17 years, 7 months ago)

i would soak mine in isopropyl for a little bit sometimes longer. then i let them dry disassembled in front of my flowhood while i do extra work. once they are dry i put them inside a spawn bag. this is for sterilizing 20+. i autoclave them for 40 min approx 15 min at 15psi. 40 min because my cooker takes a little to heat up. i open my sterilser in front of my flow hood and then let any moisture evaporate. then i seal with impulse sealer. throw on a shelf and it will stay sterile foever (or so it seems).


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OfflineMrMolotov
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Re: How do I resterilize syringes for reuse? [Re: durban_poison]
    #5768849 - 06/19/06 05:05 PM (17 years, 7 months ago)

i just fill them with water place the plunger side down on the PC basket and just PC for a hour. i usually toss them in with my jars that are loaded with grain for the sterilization


--------------------


OI OI OI


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InvisibleMasFina
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Re: How do I resterilize syringes for reuse? [Re: MrMolotov]
    #5769052 - 06/19/06 06:05 PM (17 years, 7 months ago)

Monoject Syringes can be PCed too.


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PM me if you are interested in buying 140ml syringes. $6 each + $7 shipping


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Offlinejohnuk
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Re: How do I resterilize syringes for reuse? [Re: MasFina]
    #5769111 - 06/19/06 06:17 PM (17 years, 7 months ago)

Got me a sweeeeeet glass syringe, although a gas syringe would be cooler. Can be sterilised to ~ 200C I think.




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InvisibleBlutjager
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Registered: 06/11/06
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Re: How do I resterilize syringes for reuse? [Re: johnuk]
    #5834784 - 07/08/06 11:33 AM (17 years, 6 months ago)

I need to buy some of those glass syringes


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Offlinedot_com_unist
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Re: How do I resterilize syringes for reuse? [Re: Blutjager]
    #5835224 - 07/08/06 01:44 PM (17 years, 6 months ago)

if you look in the PF TEK there is a part in there about preparing a spore syringe. I believe it also tells you how to sterilize it there.


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