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Offlineacidbuba
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Registered: 01/31/06
Posts: 125
Last seen: 16 years, 6 months
home made syringe contam ????
    #5750036 - 06/14/06 03:32 PM (17 years, 7 months ago)

ok so i have 24 jars of sterilized rye and some home made syringes i made from prints that i had from my last grow. in an attempt to test the syringes i only shot up 4 jars 3 days ago as not to rune the entire batch. so far so good all 4 jars are showing some growth, and this leads me to my question. since these are the 1st syringes that i have ever made i was wondering on average how long would it take before i would notice any contamination? i practice sterile techniques at all time and on my last grow i had about 18 casings and no contams at all. i created the spore prints by placing the caps in there own sterilized 1/2 pint wide mouth jars containing a piece of foil to seal the print for later use. then the syringes were sterilized with filtered water and a few drops of jet dry. also a shot glass for every syringes and a butter knife to scrape the prints into the shot glasses were sterilized with the syringes. i use a pressure cooker for all sterilization for at least an hour (i think i PCd the syringes and other materials for 1.5 hrs). i then used my home made glove box to put it all together.


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Offlinesporey
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Registered: 05/09/06
Posts: 11
Last seen: 17 years, 7 months
Re: home made syringe contam ???? [Re: acidbuba]
    #5750058 - 06/14/06 03:37 PM (17 years, 7 months ago)

Sounds like you have a very good method for putting syringes together, i would be surprised if you get contams.

They usually take a week or 2 to show up in liquid, and are very easy to spot. I like to inoculate a jar of sterilized honeywater to test a syringe for contams.


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