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kehoe
Stranger
Registered: 03/05/06
Posts: 19
Last seen: 15 years, 9 months
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inoculation
#5748907 - 06/14/06 08:24 AM (17 years, 7 months ago) |
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Hey,
Currently my jars are in my incubator growing mycelium, but my question is what did I do wrong because out of 8 jars, only 1 have mycelium grow on all four inoculation points. Like 5 out of the 8 jars only grew at three points. By now they are about 80% colonized, but I was just wondering what I can do next time to get it to work at all four points.
What I did was sterilize the needle for ~5 seconds or so until it turned red, waited a second without the flame, and then injected. Should I have shot some extra through the needle before I inoculated?
thanks
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bombardier420
Unrated. Now with more nudity!



Registered: 03/02/06
Posts: 70
Loc: somewhere outside Barstow...
Last seen: 13 years, 7 months
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Re: inoculation [Re: kehoe]
#5748952 - 06/14/06 08:56 AM (17 years, 7 months ago) |
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thats what i do. but, then again, with the jars i have going now (about 95% along), all four of them only started from one inoc point. just be glad you didn't have to wait that one out....lol
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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You probably heat killed the solution inside the needle and then didn't run enough solution through it to cool it down.
The needle doesn't need to get quite so hot. You can also wipe it with an alcohol wipe before injecting to cool it down.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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kehoe
Stranger
Registered: 03/05/06
Posts: 19
Last seen: 15 years, 9 months
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Re: inoculation [Re: fastfred]
#5749178 - 06/14/06 10:32 AM (17 years, 7 months ago) |
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Quote:
fastfred said: You probably heat killed the solution inside the needle and then didn't run enough solution through it to cool it down.
The needle doesn't need to get quite so hot. You can also wipe it with an alcohol wipe before injecting to cool it down.
-FF
i figured I killed it with the heat, thanks for the tip on the alcohol wipe
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: inoculation [Re: kehoe]
#5749204 - 06/14/06 10:50 AM (17 years, 7 months ago) |
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be sure to shake up your syringe real well at the beginning, and again every few jars so the spores are well distributed... especially with a light or clear syringe, there's still plenty of spores but you want to be sure some get out in every shot.
im a little doubtful that solution travelling quickly through a hot needle is going to heat the spores to a temperature that will instantly kill them, they need to be subjected to a bit of heat for a few minutes to kill them, the needle cools so quickly on its own let alone once you're squirting juice through it... but the alcohol wipe is a safe bet, and works fine on its own without flaming at all.
other than that it just happens sometimes, one missed inoculation point doesn't slow you down too much because the ones surrounding it as im sure you've observed agressively come colonizing around the corner anyway
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coda
Banjo Goiter


Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
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Re: inoculation [Re: creamcorn]
#5749289 - 06/14/06 11:14 AM (17 years, 7 months ago) |
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Heat has nothing to do with no germination at inoculation point. Don't worry if you don't see growth at all 4 points.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten)
 Don't fuck with the laughing jesus.
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