Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinekehoe
Stranger
Registered: 03/05/06
Posts: 19
Last seen: 15 years, 9 months
inoculation
    #5748907 - 06/14/06 08:24 AM (17 years, 7 months ago)

Hey,

Currently my jars are in my incubator growing mycelium, but my question is what did I do wrong because out of 8 jars, only 1 have mycelium grow on all four inoculation points. Like 5 out of the 8 jars only grew at three points. By now they are about 80% colonized, but I was just wondering what I can do next time to get it to work at all four points.

What I did was sterilize the needle for ~5 seconds or so until it turned red, waited a second without the flame, and then injected. Should I have shot some extra through the needle before I inoculated?


thanks


Extras: Filter Print Post Top
Offlinebombardier420
Unrated. Now with more nudity!
Male

Folding@home Statistics
Registered: 03/02/06
Posts: 70
Loc: somewhere outside Barstow...
Last seen: 13 years, 7 months
Re: inoculation [Re: kehoe]
    #5748952 - 06/14/06 08:56 AM (17 years, 7 months ago)

thats what i do. but, then again, with the jars i have going now (about 95% along), all four of them only started from one inoc point. just be glad you didn't have to wait that one out....lol


Extras: Filter Print Post Top
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: inoculation [Re: bombardier420]
    #5748982 - 06/14/06 09:15 AM (17 years, 7 months ago)

You probably heat killed the solution inside the needle and then didn't run enough solution through it to cool it down.

The needle doesn't need to get quite so hot. You can also wipe it with an alcohol wipe before injecting to cool it down.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


Extras: Filter Print Post Top
Offlinekehoe
Stranger
Registered: 03/05/06
Posts: 19
Last seen: 15 years, 9 months
Re: inoculation [Re: fastfred]
    #5749178 - 06/14/06 10:32 AM (17 years, 7 months ago)

Quote:

fastfred said:
You probably heat killed the solution inside the needle and then didn't run enough solution through it to cool it down.

The needle doesn't need to get quite so hot. You can also wipe it with an alcohol wipe before injecting to cool it down.


-FF




i figured I killed it with the heat, thanks for the tip on the alcohol wipe


Extras: Filter Print Post Top
Invisiblecreamcorn
mad scientist
Male User Gallery

Registered: 03/13/06
Posts: 2,962
Trusted Cultivator
Re: inoculation [Re: kehoe]
    #5749204 - 06/14/06 10:50 AM (17 years, 7 months ago)

be sure to shake up your syringe real well at the beginning, and again every few jars so the spores are well distributed... especially with a light or clear syringe, there's still plenty of spores but you want to be sure some get out in every shot.

im a little doubtful that solution travelling quickly through a hot needle is going to heat the spores to a temperature that will instantly kill them, they need to be subjected to a bit of heat for a few minutes to kill them, the needle cools so quickly on its own let alone once you're squirting juice through it... but the alcohol wipe is a safe bet, and works fine on its own without flaming at all.

other than that it just happens sometimes, one missed inoculation point doesn't slow you down too much because the ones surrounding it as im sure you've observed agressively come colonizing around the corner anyway :smile:


Extras: Filter Print Post Top
Offlinecoda
Banjo Goiter
Male

Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
Trusted Cultivator
Re: inoculation [Re: creamcorn]
    #5749289 - 06/14/06 11:14 AM (17 years, 7 months ago)

Heat has nothing to do with no germination at inoculation point. Don't worry if you don't see growth at all 4 points.


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Sterilizing with Heat or Fire, versus wiping with alcohol (needle and others) Anonymous 15,251 8 01/14/05 03:56 PM
by Anonymous
* inoculating birdeed needle wont reach sides Northernsoul 1,102 5 01/25/02 01:26 AM
by Northernsoul
* water content vs injection amount. Brady 796 6 11/03/04 11:02 PM
by scatmanrav
* does alcohol kill spores jalfonso 10,855 15 08/21/02 09:33 AM
by Anonymous
* Easy to make Inoculation hood lysergic 6,542 11 07/10/03 03:58 PM
by lysergic
* v.low risk syringe injection technique. doktor_alternate 1,457 8 11/26/03 04:35 AM
by YesItsMe
* Keeping needles clean made easy. Magash 1,635 11 04/08/04 03:04 PM
by mickywilliams2
* Injecting water? RedeyedElf 1,005 2 04/11/02 12:25 AM
by sixpac440

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
538 topic views. 31 members, 217 guests and 41 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.008 seconds on 14 queries.