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Offlinehippie_cune
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Registered: 06/13/06
Posts: 166
Last seen: 16 years, 4 months
Double Inoculation
    #5748409 - 06/14/06 02:41 AM (17 years, 7 months ago)

is it possible to inoculate my cakes with more spores after ive been letting the jars sit for a few days? what would happen?

i know this seems like it may be a hasty way of tryin to cultivate the jars quicker, but a friend of mine asked, and i told him id ask here...

so here i am.

thanks


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Offlinedreadful
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Re: Double Inoculation [Re: hippie_cune]
    #5748553 - 06/14/06 03:58 AM (17 years, 7 months ago)

So you've already inoculated them, and you're considering inoculating them again?

I'm not sure if it would speed things up at all. The spores that have been in there for a few days are that much closer to germination, so they would probably get going before the new ones anyway. Probably a bad idea; the more needles you stick into the jars, the more likely they are to get contaminated.


--------------------
It's a grim kind of celebration, a dance on razor's edge ...
-- Scott O. Moore


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OfflineIGnosticAbhorI
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Registered: 11/06/04
Posts: 4,899
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Re: Double Inoculation [Re: dreadful]
    #5748679 - 06/14/06 05:25 AM (17 years, 7 months ago)

No it won't speed things up. It will just put more moisture in the jar.

If you want to speed up colonization, inoculate with an LC next time instead of multi-spore.

-Gnostic


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Offlinebombardier420
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Re: Double Inoculation [Re: IGnosticAbhorI]
    #5748971 - 06/14/06 09:08 AM (17 years, 7 months ago)

i had a bunk syringe of oxaca. re innoc'd (after several weeks mind you), and had success with my pesa syringe. in my (limited) experience, it will work. however, it is an uneccesary risk at this point. give it lots of time. if you see nothing after several weeks, including no contams, i say you might as well give it a shot. thats what i did. figured i had nothing to loose at that point anyways...


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Offlinebombardier420
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Re: Double Inoculation [Re: bombardier420]
    #5748976 - 06/14/06 09:11 AM (17 years, 7 months ago)

i may have answered your question wrong. if your just looking to speed things up, listen to IGnostic ^. if you feel your syringe is shootin blanks (and its only been a couple days, so how could you know that...??), then gofer it


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Offlinehippie_cune
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Registered: 06/13/06
Posts: 166
Last seen: 16 years, 4 months
Re: Double Inoculation [Re: bombardier420]
    #5749591 - 06/14/06 12:55 PM (17 years, 7 months ago)

ok thanks guys..

i dont think i was gonna do it.. my nextdoor neighbor(and best friend..we live in a duplex) we were baked and he just starts flowin out these questions.. most of em i knew the answer, but when that came up i didnt know...

id never even read anything about a second innoc.

Im still a NOOB so, what is a LC and a multi-spore?


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OfflineIGnosticAbhorI
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Re: Double Inoculation [Re: hippie_cune]
    #5749640 - 06/14/06 01:09 PM (17 years, 7 months ago)

Multi-spore = Syringe with spores in it.

LC = A liquid culture with mycelium in it, used to inoculate sub. with.

Easy LC tek for ya

:cheers:

-Gnostic


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Offlinehippie_cune
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Registered: 06/13/06
Posts: 166
Last seen: 16 years, 4 months
Re: Double Inoculation [Re: IGnosticAbhorI]
    #5749718 - 06/14/06 01:38 PM (17 years, 7 months ago)

thanks alot!!

btw.. the honey? what kind is best? should i try n find the purest honey i can.. or is it ok with perservatives?


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Offlinekilroy
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Re: Double Inoculation [Re: hippie_cune]
    #5749772 - 06/14/06 01:56 PM (17 years, 7 months ago)

go all natural if you can


--------------------
IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





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Offlinehippie_cune
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Registered: 06/13/06
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Re: Double Inoculation [Re: kilroy]
    #5749837 - 06/14/06 02:22 PM (17 years, 7 months ago)

...

i know of a beehive....

should i get that?


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OfflineTS10
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Registered: 11/02/05
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Re: Double Inoculation [Re: hippie_cune]
    #5749944 - 06/14/06 03:04 PM (17 years, 7 months ago)

Quote:

hippie_cune said:
...

i know of a beehive....

should i get that?




As long as you have a friend w/ a vidcam recording it, sure. :wink:


--------------------
Gee Elsie, how do you do it?


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OfflineOatman2000
-=Outa Space=-
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Re: Double Inoculation [Re: TS10]
    #5750009 - 06/14/06 03:26 PM (17 years, 7 months ago)

LOL.... i second that


--------------------
Spawning to COIR
:thumbup:  My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION
----------------------------

4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine


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Offlinehippie_cune
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Registered: 06/13/06
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Last seen: 16 years, 4 months
Re: Double Inoculation [Re: Oatman2000]
    #5750179 - 06/14/06 04:19 PM (17 years, 7 months ago)

hahaha

i would, but my cams battery is out and my bro lost the charger like 2 years ago :frown:

seriously.. would it be better than store bought "pure honey" or would store bought be better cause maybe its cleaner?


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Offlineslitheen
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Re: Double Inoculation [Re: hippie_cune]
    #15302988 - 10/31/11 08:56 AM (12 years, 2 months ago)

Quote:

hippie_cune said:
hahaha

i would, but my cams battery is out and my bro lost the charger like 2 years ago :frown:

seriously.. would it be better than store bought "pure honey" or would store bought be better cause maybe its cleaner?




At the risk of being called a thread necromancer, I'd just like to say... Wooosh!


--------------------
"Take all that money we spend on weapons and defences each year and instead spend it feeding and clothing and educating the poor of the world, which it would pay for many times over, not one human being excluded, and then we could explore space, together, both inner and outer... forever... in peace."
Bill Hicks (Comedian, wise man and fellow psychonaut).



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OfflineDodongo
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Registered: 10/16/11
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Re: Double Inoculation [Re: hippie_cune]
    #15303870 - 10/31/11 01:30 PM (12 years, 2 months ago)

I just did this, in my first grow no less, and it seems to be working so far. Made up some jars of WBS and inoculated with a free syringe I got from another member, waited for two weeks and no sign of anything. I made up a few syringes of my own from a large and dark print, re-inoculated a few jars and after only a few days saw signs of growth.

Now a couple days later, there's tons of mycelium up and down each side that I shot the new syringe.

If your sterilization technique is good, I'd say it's safe. But who knows, maybe I threw off the moisture content by doing this and will not achieve full colonization? I'll keep you posted.


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun


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OfflineDodongo
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Registered: 10/16/11
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Re: Double Inoculation [Re: slitheen]
    #15303884 - 10/31/11 01:33 PM (12 years, 2 months ago)

Wow, didn't realize it was such an old thread.. any reason for bringing it back?


--------------------
And you may see me tonight with an illegal smile
It don't cost very much, but it lasts a long while
Won't you please tell the man I didn't kill anyone
No I'm just tryin' to have me some fun


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