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Invisibleshroomerite
Apprentice

Registered: 06/09/06
Posts: 513
Incubating LC's *DELETED*
    #5746936 - 06/13/06 07:23 PM (17 years, 7 months ago)

Post deleted by shroomerite

Reason for deletion: n



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  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Invisibleshroomerite
Apprentice

Registered: 06/09/06
Posts: 513
Re: Incubating LC's *DELETED* [Re: shroomerite]
    #5746983 - 06/13/06 07:32 PM (17 years, 7 months ago)

Post deleted by shroomerite

Reason for deletion: n



--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Offlinesplifner180
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Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
Re: Incubating LC's [Re: shroomerite]
    #5747492 - 06/13/06 09:42 PM (17 years, 7 months ago)

I'd suggest 1-3cc of spore solution for a 1 qt jar.

As far as the LC myc growth, maybe a cloud the size of a few green grapes? If you shake it before you aspirate the LC with a syringe you can break it up well. Even better is to use a magnetic stirrer becaues then the solution is very slurry-ish and you're guaranteed to get myc into every jar you use that syringe on.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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InvisibleTippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
Re: Incubating LC's [Re: shroomerite]
    #5747559 - 06/13/06 10:01 PM (17 years, 7 months ago)


6 or 7 days @ 78/80F did this. :grin:


--------------------
Perfection is attained by slow degrees; it requires the hand of time...
[


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Invisibleshroomerite
Apprentice

Registered: 06/09/06
Posts: 513
Re: Incubating LC's *DELETED* [Re: splifner180]
    #5747562 - 06/13/06 10:02 PM (17 years, 7 months ago)

Post deleted by shroomerite

Reason for deletion: c



--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Invisibleshroomerite
Apprentice

Registered: 06/09/06
Posts: 513
Re: Incubating LC's *DELETED* [Re: shroomerite]
    #5747653 - 06/13/06 10:20 PM (17 years, 7 months ago)

Post deleted by shroomerite

Reason for deletion: c



--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Offlinesplifner180
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Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
Re: Incubating LC's [Re: shroomerite]
    #5747787 - 06/13/06 10:58 PM (17 years, 7 months ago)

Nice job on the LC jars but I'm a bit concerned about the tubing. That looks like polyvinyl or something. That won't melt while PCing? Also, how did you seal the tube to the lid?

Good deal on the magstirrers. I LOVE mine.

shroomerite writes:
"How many quarts of WBS could I knock up with 4 oz of LC?"

4oz is over 100cc. If you figure each qt jar needs only two, maybe three cc each, a few dozen.

"Also does anyone know where to get longer needles for my syringe? "

Can't help you there but with that kind of jar setup you can just aspirate LC by tilting it to the side.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Invisibleshroomerite
Apprentice

Registered: 06/09/06
Posts: 513
Re: Incubating LC's *DELETED* [Re: splifner180]
    #5747807 - 06/13/06 11:04 PM (17 years, 7 months ago)

Post deleted by shroomerite

Reason for deletion: c



--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Invisibleshroomerite
Apprentice

Registered: 06/09/06
Posts: 513
Re: Incubating LC's [Re: shroomerite]
    #5747820 - 06/13/06 11:08 PM (17 years, 7 months ago)

eww, it says its vinyl tubing. Will that melt? or just get soft?


--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Invisiblefastfred
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Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: Incubating LC's [Re: shroomerite]
    #5747842 - 06/13/06 11:13 PM (17 years, 7 months ago)

> I'd suggest 1-3cc of spore solution for a 1 qt jar.

That's a waste of spore solution. 1 drop is plenty. Each drop contains thousands of spores, which is more than enough. You would do fine to use even less than that, but there's no easy way to measure less than one drop.

The more you inject the higher your chances of contamination unless you have a perfectly clean spore syringe, which is unlikely. Even good "uncontaminated" spore syringes will likely have a stray contam or two floating in them. The more you inject the more likely that you'll inject one of the few contams in a syringe.

As for longer needles... I wish you could get them easily, but I haven't ever found any. 1.5 inch is pretty standard and about the longest I've been able to find. You can get a cannula, which can be pretty long. I've seen those as long as 4-5 inches. They work great.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


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Invisibleshroomerite
Apprentice

Registered: 06/09/06
Posts: 513
Re: Incubating LC's *DELETED* [Re: fastfred]
    #5747872 - 06/13/06 11:22 PM (17 years, 7 months ago)

Post deleted by shroomerite

Reason for deletion: c



--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Invisibleshroomerite
Apprentice

Registered: 06/09/06
Posts: 513
Re: Incubating LC's [Re: shroomerite]
    #5747950 - 06/13/06 11:54 PM (17 years, 7 months ago)

OK, so I did a test. I put about 1.5 inches of water in a med size saucepan and boiled on HIGH for 15 min. The tubing was soft but still very strong. I see no problems with PC'ing the jars. Yay for me :smile: as those took me a while to make and I have no other tubing to use.


--------------------
  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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Offlinesplifner180
Student
 User Gallery

Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
Last seen: 1 year, 3 months
Re: Incubating LC's [Re: shroomerite]
    #5748823 - 06/14/06 07:32 AM (17 years, 7 months ago)

shroomerite writes:
"OK, so I did a test. I put about 1.5 inches of water in a med size saucepan and boiled on HIGH for 15 min. The tubing was soft but still very strong. I see no problems with PC'ing the jars. Yay for me as those took me a while to make and I have no other tubing to use."

Frankly, I'm more concerned about toxicity.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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