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Ascension
Stranger

Registered: 01/13/05
Posts: 156
Loc: Still trying to work it o...
Last seen: 17 years, 7 months
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Not PC'ing LC causes no growth?
#5743381 - 06/12/06 07:55 PM (17 years, 7 months ago) |
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Ive been having heaps of trouble with LC's, I think it may be because im using tap water so ive just knocked up some more using filtered water.
But because ive been having heaps of trouble im not bothering to put them in the PC because I just want to get a method down that works. Once I get my method working then ill do a proper sterelized batch, but my concern is, could in any would not PC'ing the LC jars cause them not to grow?
I highly doubt it, but just incase I got to ask
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Liquidkick
H2O
Registered: 05/03/02
Posts: 2,635
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Re: Not PC'ing LC causes no growth? [Re: Ascension]
#5743399 - 06/12/06 07:58 PM (17 years, 7 months ago) |
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I use tap water for everything, and i pc them.
I think you need to sterilize the LC before you try and knock them up with spores.
Thats how i do it.
MUST STERILIZE!!!
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Not PC'ing LC causes no growth? [Re: Ascension]
#5743411 - 06/12/06 08:00 PM (17 years, 7 months ago) |
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you'll get growth if you dont PC, you just wont like what comes from it, dont waste the spores, give us more info like the amount of time in the PC the amount of culture media to water and so on.
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JoeMama
Registered: 04/28/06
Posts: 154
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Re: Not PC'ing LC causes no growth? [Re: Liquidkick]
#5743418 - 06/12/06 08:01 PM (17 years, 7 months ago) |
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I used plastic microwavable containers with Karo syrup and plastic wrap on the tops (with the lid over that) and micrwave them for 5 minutes. I havent had an issue yet. If you do PC them, dont do it for very long. You run the chance of the sugars carmelizing. -Me
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liamtheloser
Advanced Idiot

Registered: 06/07/06
Posts: 1,453
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Re: Not PC'ing LC causes no growth? [Re: JoeMama]
#5743741 - 06/12/06 09:12 PM (17 years, 7 months ago) |
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if you don't sterlize (microwave/boil/pc) then you're going to get either bacterial or fungal contams... or possibly both. that's one sure way to ruin some good spores.
i just boil a jar with 2 holes in it, pull it out with tongs, put a little honey until it tints the water slightly yellow, then screw the lid on, put foil over it, and boil it for about 30 minutes. worked wonders, i have a jar right now that's 30% colonized after 4 days.
don't forget to incubate your LC, that could be another problem you're having.
oh... and if you're using JUST tap water, with no honey/karo/molasses/mae, then you're probably just going to have a jar of contam'd spore that will never colonize... because spores need simple carbohydrates to feed on.
-2cents
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