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Offlinephungi
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Registered: 06/03/06
Posts: 219
Loc: Canada (not really)
Last seen: 17 years, 2 months
Newbie...plagued with contams
    #5740863 - 06/12/06 07:27 AM (17 years, 7 months ago)

O.K. out of 21 pf jars, I think that maybe 2 or 3 are not contaminated, the rest are not so hot. I'm 8 days from inoculation, and I have quite a bit of mycelium germination, but many are sickly looking and I have a lot of reds and pinks.....bummer right?

I did my sterile work in the kitchen, and I was very carful, but this is my first time.

I am going to create a new space and inoculate agian, I have an ozone generator, and a pressure cooker. And I can build a glove box if nessecary. I have 2 more syringes.

I am fishing for advise on setting my new "lab" I guess, cause I followed all the advise on the various pf teks which are supposed to be almost fool proof. I hope that I'm not a fool.

Seriously, I've got all the tools, and I am patient, I'm not in a hurry, I just really want to get it right. These are the types of hobbies that I live for!!

The mycelium that I have produced (little though it may be) is very exciting to me.

Phungi


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OfflineMLBjammer
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Registered: 02/24/06
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Re: Newbie...plagued with contams [Re: phungi]
    #5740916 - 06/12/06 08:21 AM (17 years, 7 months ago)

How did you inoculate? That's the most common way to invite contams into a jar. If you didn't inoculate in a still air space, with lots of alcohol for the syringe needle, that may be your problem. But a glove box is a good idea and will come in handy for lots of other tasks. But just retrace how you followed the tek to figure out where you erred. And there is an off-chance that your syringes were contam'ed. At least you got some mycelium going. GL.


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Offlinephungi
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Registered: 06/03/06
Posts: 219
Loc: Canada (not really)
Last seen: 17 years, 2 months
Re: Newbie...plagued with contams [Re: MLBjammer]
    #5741080 - 06/12/06 09:24 AM (17 years, 7 months ago)

yea I'm stoked about the mycelium. Even if I harvest no usable materials at all off of this run, I've learned some lessons already and I am now on the learning curve, gotta love it. plus i've gathered all the shit that i need so now its just a matter of working out the details. Thanx for the response.

phungi


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"Don't Steal. The government hates competition!!"


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Offlinephungi
BullshitDetector

Registered: 06/03/06
Posts: 219
Loc: Canada (not really)
Last seen: 17 years, 2 months
Re: Newbie...plagued with contams [Re: phungi]
    #5741098 - 06/12/06 09:35 AM (17 years, 7 months ago)

Oh yea, to answer your question. I followed all recamended sterile tech in my kitchen, covered myself properly,(resperator, rubber gloves,shower cap,) flamed the needle red hot, and used about 1/4 cc per hole. Flamed the needle about every 4th jar, and did 21 jars, 2 syringes worth. oh yea i kept 2 jars as a control, nothing growing in them at all. If all of my jars are contaminated I will be thinking that the contam came in the syringes. But we'll see. I'll wait a week before i give it to much more thought.

phungi.


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OfflineRogerRabbitM
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Re: Newbie...plagued with contams [Re: phungi]
    #5742480 - 06/12/06 04:27 PM (17 years, 7 months ago)

Reds and pinks are definitely contamination. A kitchen is the worst place I can think of to do sterile work. There is more bacteria and fecal matter in a kitchen than your toilet. Clean out a closet to use, or better yet, build a still air glovebox. Flame your needle red hot, then insert it into the jar while still red hot. The verm and substrate will cool it most of the way, and the first few drops of solution will finish cooling the needle, so the rest of your spore solution can flow contaminant free.
RR


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