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Offlinethink_about_it
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Registered: 05/21/06
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How long after inoculation before you peek? *DELETED*
    #5737710 - 06/11/06 11:47 AM (17 years, 7 months ago)

Post deleted by think_about_it

Reason for deletion: No need.



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InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
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Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
Re: How long after inoculation before you peek? [Re: think_about_it]
    #5737737 - 06/11/06 11:55 AM (17 years, 7 months ago)

You wont see anything for a minimum of 6 days.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlinethink_about_it
Stranger
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Registered: 05/21/06
Posts: 107
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Re: How long after inoculation before you peek? *DELETED* [Re: Psychoslut]
    #5737751 - 06/11/06 12:00 PM (17 years, 7 months ago)

Post deleted by think_about_it

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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: How long after inoculation before you peek? [Re: think_about_it]
    #5737795 - 06/11/06 12:15 PM (17 years, 7 months ago)

you can look at your jars all you want.

looking changes the temperature in your incubator though.
The light won't hurt them.

So keep it closed and look once every week or so.
If you are impatient and what not, buy more jars and do
another batch to keep you occupied.
spend some time researching.

wait, then wait some more.

If you don't want to wait so long, make a good Liquid Culture.
It could easily cut your colonization time in half if done right.

You'll know a contam if you see one.
mycelium is white.
contams are everything but white.
only hope for white.

If you did things correctly you should have no contams, so don't worry about them yet. Just worry about remaining sterile.


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
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Re: How long after inoculation before you peek? [Re: think_about_it]
    #5739134 - 06/11/06 06:52 PM (17 years, 7 months ago)

Keeping them in the dark is a good lesson in patience and proper technique. If all the newbies were to follow their own advice there yields would drop off considerably through pure ignorance. Of course light isn't going to stop myc growth but you do loose a VERY USEFUL TRIGGER. Peeking every few days is fine keep fanning durations short (15-20 seconds), invitro pinning usually occurs after full colonization anyway which (full colonization) is a survival trigger which can be used with great success when combined with your other pinning triggers at the exact same time. GL


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinethink_about_it
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Registered: 05/21/06
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Re: How long after inoculation before you peek? *DELETED* [Re: hyphae]
    #5744862 - 06/13/06 03:55 AM (17 years, 7 months ago)

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Offlinedreadful
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Registered: 05/27/06
Posts: 24
Loc: SF bay area
Last seen: 17 years, 4 months
Re: How long after inoculation before you peek? [Re: think_about_it]
    #5744874 - 06/13/06 04:15 AM (17 years, 7 months ago)

In my very limited experience, I've sometimes seen germination in less than six days. Other times it's taken longer. Just sit tight =)

If you are really antsy waiting, why not mix up and inoculate another batch? Each batch takes such a god awful long time throughout its whole life cycle (incubation, waiting for pins, first flush, waiting again, etc.), it is nice to have several going at once to keep yourself amused. It won't be so painful to always be waiting.


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It's a grim kind of celebration, a dance on razor's edge ...
-- Scott O. Moore


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Offlinethink_about_it
Stranger
Male
Registered: 05/21/06
Posts: 107
Last seen: 16 years, 10 months
Re: How long after inoculation before you peek? *DELETED* [Re: dreadful]
    #5744875 - 06/13/06 04:18 AM (17 years, 7 months ago)

Post deleted by think_about_it

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OfflineMLBjammer
Invitro Freak
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Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 14 days
Re: How long after inoculation before you peek? [Re: think_about_it]
    #5744906 - 06/13/06 05:01 AM (17 years, 7 months ago)

I usually see some growth about day 4 or 5. Don't worry till about two weeks, then start over. Keep the jars at 80 degrees F.


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