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OfflineDucky
Governor ofRhythm
Male

Registered: 05/09/06
Posts: 71
Loc: on a boat somewhere
Last seen: 17 years, 5 months
Shitty syringe
    #5736198 - 06/10/06 11:12 PM (17 years, 7 months ago)

6 pint jars, BRF substrate, EQ, over a week at 84 deg., nothing.

No signs of contamination, no signs of anything! Zip. WTF?? Impatient, maybe, but this seems lame. Little dark clumps of spores are even visible stuck to the side of the glass in places, so they def. got in there. Anyone ever heard of fake syringes? Dead/expired syringes? I'm posting for a friend btw..


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"Whoa! ...did you just see that too?"


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OfflineKryptik
Enjoy the Magic

Registered: 04/05/06
Posts: 136
Last seen: 9 years, 3 months
Re: Shitty syringe [Re: Ducky]
    #5736341 - 06/10/06 11:48 PM (17 years, 7 months ago)

There are a lot of factors that could effect this...so there is no direct answer. Wait another week, then trash them. If the Syringe is from a Shroomery sponsor, I wouldn't worry about it being "shitty" /bunk.

If BRF is made wrong, innoc'd when hot, innoc'd in the center......it can either have killed or stunted the growth...


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Hex45 78 70 6c 6f 72 65 20 74 68 65 20 4d 61 67 69
63 2e 20 4b 69 6c 6c 20 79 6f 75 72 20 65 67 6f 21


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OfflineFeelers
Anti-Myth-Rhythm-Rock-Shocker
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Registered: 06/18/02
Posts: 1,806
Loc: Land of Oz
Last seen: 5 years, 8 months
Re: Shitty syringe [Re: Kryptik]
    #5736375 - 06/10/06 11:57 PM (17 years, 7 months ago)

Make sure your temp reading is accurate. You could be cooking them perhaps? I did this and didnt see any growth for ages. Ask the sponsor about it.


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Offlinekilroy
Hightimes
Male

Folding@home Statistics
Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit Flag
Last seen: 13 years, 3 months
Re: Shitty syringe [Re: Feelers]
    #5736574 - 06/11/06 12:31 AM (17 years, 7 months ago)

My first jars took 2 weeks before I saw anything in them. if you made the jars aliitle on the damp side or if yuo packed the to much then these things will also slow it down. Relax and be patient, we all have gone through this but you are not screwed yet by any means.


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IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





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OfflineFestivus
Resident Lurker
Male

Registered: 03/24/06
Posts: 142
Last seen: 15 years, 1 month
Re: Shitty syringe [Re: kilroy]
    #5736654 - 06/11/06 12:47 AM (17 years, 7 months ago)

BRF jars can take a while, especially with spore innocs. Next time, make an LC and stand back! :smile:

Wait another week and see how it goes. For now, I wouldn't worry about it too much.


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InvisibleAtheist
Stranger
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Registered: 01/24/06
Posts: 13,705
Loc: USA
Re: Shitty syringe [Re: Festivus]
    #5736844 - 06/11/06 01:29 AM (17 years, 7 months ago)

give them time

you shouldv done a liquid culture

PM me if you have any questions :thumbup:


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Offlinefrankthetank87
THE TANK
Registered: 07/01/05
Posts: 120
Last seen: 16 years, 10 months
Re: Shitty syringe [Re: Atheist]
    #5737372 - 06/11/06 09:41 AM (17 years, 7 months ago)

hey bro I know someone who did the same exact thing, it may take 5 weeks to complete the process. his pint jars took about 2 weeks, maybe a few days over. same strain/substrate too.


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-frank the tank-
Anything said above by the user frankthetank87 is completely fictional and used for entertainment purposes only.


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Offlinegyzmo
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Registered: 05/03/06
Posts: 37
Last seen: 16 years, 4 months
Re: Shitty syringe [Re: frankthetank87]
    #5738501 - 06/11/06 04:06 PM (17 years, 7 months ago)

Can I have my syringe stored at room temp for example half a year? or must have in a fridge


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Invisibletoole
white-thumb (Onewhackmycophiliac)
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Folding@home Statistics
Registered: 05/01/06
Posts: 500
Loc: spore #1203 - bas 2.34 - ...
Re: Shitty syringe [Re: gyzmo]
    #5738509 - 06/11/06 04:07 PM (17 years, 7 months ago)

I kept a syringe at room temp-even small flucuations of above average temps for about 4 to 5 months and it still worked just fine.


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-the adventures of suse and prescott.9-

..and the neverending triscut of doom !


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Offlinecoda
Banjo Goiter
Male

Registered: 03/20/01
Posts: 8,750
Last seen: 10 months, 3 days
Trusted Cultivator
Re: Shitty syringe [Re: toole]
    #5738731 - 06/11/06 05:01 PM (17 years, 7 months ago)

#1 rule to mycology

learn some fucking patience!  A week is not a long time to be waiting, some people dont see germination until about the second week, others see a month before any signs.

There are way too many factors to determine the problem, on average BRF cakes perform poorly because of poor water content or compacted substrate.  It's not uncommon to see long colonization times when following the PF method.  At the 2nd week mark it's a good idea to get some other jars whipped up, if the first grow fails you have others on the way and won't lose much time.

good luck :smile:


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To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
Trusted Cultivator
Re: Shitty syringe [Re: Ducky]
    #5739408 - 06/11/06 08:01 PM (17 years, 7 months ago)

As coda said and I'll reiterate first lesson in mycology is "Patience is a Virtue"! Germination can often take up to 3 weeks, personally I would not wait any longer than that as my time will be put to better use. GL bro


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineDucky
Governor ofRhythm
Male

Registered: 05/09/06
Posts: 71
Loc: on a boat somewhere
Last seen: 17 years, 5 months
Re: Shitty syringe [Re: hyphae]
    #5739618 - 06/11/06 09:12 PM (17 years, 7 months ago)

Wow, thanks for all the responses!  I've just gotten back after a day of recovery at an all night party at a rad club..good house music.  I totally agree that patience is virtue, I just haven't learned any yet :wink:  Don't think it's a temp. issue..the jars cooled for about 16 hours, overnight and while at work the next day.  Substrate was hardly tamped down, like really not more than a quarter inch, maybe 3/8 inch.  Water content basically what most BRF guides suggest, possibly 5-8% higher...don't think that would make a negative difference.  I will be patient. :whistling:

ps hyphae: $2/lb oyster shell flour = ripoff!!  Dude, my ladyfriend works at a little nursery owned by this rad guy who's got almost every psychotropic plant (that's legal) that grows in my region.  He sells it for 50 cents a lb, and bat guano for like 1.50/lb, lots of shiet, look around, you can find a better deal!


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"Whoa! ...did you just see that too?"


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Offlinehyphae
born to grow
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Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
Trusted Cultivator
Re: Shitty syringe [Re: Ducky]
    #5740809 - 06/12/06 06:12 AM (17 years, 7 months ago)

50 cents a pound would hardly cover costs for an online business besides it does go a long way. If you have a cheaper online source post it otherwise please she isn't ripping off anyone she is providing a service at a reasonable price IMO.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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