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OfflineDestroyerOWorlds
Stranger

Registered: 11/28/05
Posts: 60
Last seen: 14 years, 8 months
Spores didnt germinate, can I reuse the substrate *DELETED* *DELETED*
    #5733111 - 06/10/06 02:45 AM (17 years, 9 months ago)

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InvisibleJoeMama
Registered: 04/28/06
Posts: 154
Re: Spores didnt germinate, can I reuse the substrate [Re: DestroyerOWorlds]
    #5733121 - 06/10/06 02:48 AM (17 years, 9 months ago)

I would say ditch the jars and make a new batch of PF. If you injected, then reinjected chances are you have jacked the moisture level within the jars. Give it a total of 2 weeks before calling it a total lost cause. I had this problem too but waiting seemed to be the answer. (For me anyways)
GL
-Me

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Invisibletoole
white-thumb (Onewhackmycophiliac)
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Registered: 05/01/06
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Loc: spore #1203 - bas 2.34 - ...
Re: Spores didnt germinate, can I reuse the substrate [Re: JoeMama]
    #5733204 - 06/10/06 03:39 AM (17 years, 9 months ago)

Reinjecting = Good way to contaminate your lot.

Don't get lazy with this, you'll just end up another victim to contams for hasty moves.

I learned very fast that if one step is stalled then just tuck it away(speaking of jars, placing them away in a warm, dark spot, like an unused drawer) and work on another mushie project.  You'll end up finding the jars weeks and months down the road, you'll have forgotten about them and you'll have 6 colonized jars ready to go..or 6 jars of green goop :laugh:

Either way, you just have to keep in mind that you can't get stuck waiting on some jars that arn't doing anything-flip them and if after a few more days/weeks whatever, they don't show signs of mycelium growth, either place them aside or reuse the jars.


--------------------
-the adventures of suse and prescott.9-

..and the neverending triscut of doom !

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OfflineDestroyerOWorlds
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Registered: 11/28/05
Posts: 60
Last seen: 14 years, 8 months
Re: Spores didnt germinate, can I reuse the substrate *DELETED* [Re: toole]
    #5733260 - 06/10/06 04:41 AM (17 years, 9 months ago)

Post deleted by DestroyerOWorlds

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Invisibletoole
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Registered: 05/01/06
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Loc: spore #1203 - bas 2.34 - ...
Re: Spores didnt germinate, can I reuse the substrate [Re: DestroyerOWorlds]
    #5733271 - 06/10/06 04:49 AM (17 years, 9 months ago)

I believe "whater" is water? Haha :wink:

Well in adding that water, I'm not sure if THAT amount will hurt the situation, but as the mousture levels are very important I'd doubt that adding water would be a smart thing to do-nor one to improve mycelia growth.

Did you start from scratch with new substrate in your cleaned jars?  Or just 'resterilize' the entire project?


--------------------
-the adventures of suse and prescott.9-

..and the neverending triscut of doom !

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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 month
Re: Spores didnt germinate, can I reuse the substrate [Re: toole]
    #5733330 - 06/10/06 06:06 AM (17 years, 9 months ago)

Depend's on what substrate you are useing, it may have contained fungicide's that are killing off your spores before they even have a chance to germinate.

And yes, you can re-use jar's you just need to re-sterilize them prior to re-innoculating them. Although you'll have lost abit of moisture it wont matter if your useing it as spawn.

Take care :smile:


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl

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OfflineDestroyerOWorlds
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Registered: 11/28/05
Posts: 60
Last seen: 14 years, 8 months
Re: Spores didnt germinate, can I reuse the substrate *DELETED* [Re: toole]
    #5733333 - 06/10/06 06:08 AM (17 years, 9 months ago)

Post deleted by DestroyerOWorlds

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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 month
Re: Spores didnt germinate, can I reuse the substrate [Re: DestroyerOWorlds]
    #5733337 - 06/10/06 06:20 AM (17 years, 9 months ago)

Not if you re-sterilized your jars prior to re-innoculating, what kind of substrate are you useing?


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl

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OfflineDestroyerOWorlds
Stranger

Registered: 11/28/05
Posts: 60
Last seen: 14 years, 8 months
Re: Spores didnt germinate, can I reuse the substrate *DELETED* [Re: DestroyerOWorlds]
    #5733369 - 06/10/06 07:15 AM (17 years, 9 months ago)

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OfflineMLBjammer
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Registered: 02/24/06
Posts: 560
Loc: Southeastern USA
Last seen: 7 years, 2 months
Re: Spores didnt germinate, can I reuse the substrate [Re: DestroyerOWorlds]
    #5733472 - 06/10/06 08:23 AM (17 years, 9 months ago)

Are you sure that your spore source is not the problem? Try another source--syringe, print, or whatever. And make some more jars--older jars have less life in them--re-injecting spore water or plain water is just inviting contams into your jars. GL.

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OfflineDestroyerOWorlds
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Registered: 11/28/05
Posts: 60
Last seen: 14 years, 8 months
Re: Spores didnt germinate, can I reuse the substrate *DELETED* [Re: MLBjammer]
    #5735159 - 06/10/06 06:40 PM (17 years, 9 months ago)

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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Spores didnt germinate, can I reuse the substrate [Re: DestroyerOWorlds]
    #5735181 - 06/10/06 06:50 PM (17 years, 9 months ago)

I say just wait it out.

a quick way to test viability of spores is with a Liquid Culture
or with Agar.

Use one or the other to test your spores out.
give jars 2 weeks for germination.
your problem is probably too high of an incubation temp.

just a guess of course.


--------------------


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