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Offlinesoma_seeker
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First Attempt - PF Tek
    #5733070 - 06/10/06 02:25 AM (17 years, 7 months ago)

This is my first attempt at growing after a mate of mine took it up a few months back and had success. I sampled the fruits of his labour and new i had to get involved.

since then i've sourced everything i need for my grow (using PF tek);

- container in container (with aquarium heater) incubator
- large rubbermaid style terranium
- humidified by perlite and a large air-stone that runs the entire diagonal length of the base connected at each end to a nice strong aqurium pump.

I then went to my friend to obtain some spore prints to make some LC and at the same time he offered me a PF style cake (which he didn't need as he was packing up his grow for a while) that was ready to be birthed. this cake had been inoculated 6-7 wks earlier and still was not completely colonized, he doesn't use an incubator.

so i went and made my LC and setup my terranium (without the airstone) then birthed the cake. several hrs after birthing i removed it (picked me as a noob yet?) and dunked the cake for 14hrs and rolled it in oven-cooked verm, then misted, then back into the terranium.

it sat in there for 9 days b4 i began to worry that the humidity may not be high enough to induce pinning so i went out and bought the airstone and installed it. 3 further days have passed and still the cake shows no change.

what i want to know is, do u think it is just this cake is not pinning due to its age or do u think i need to alter the conditions in my terranium?? i've been using a digital hygrometer that can only read up to 90% RH and it takes an hr or so after being placed in the terranium to reach this value and the avg temp would be about 68F.

i'm not too phased if i get nothing off this cake but i want to know the terranium is adequate for when MY cakes are ready to be birthed. then again if u think i could salvage it in anyway i'll be more than happy to give it a go.

first pic is straight after birthing, subsequent pics are from today (12 days later)

thanks for any help u can offer.

Confused Noob












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Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!


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OfflineMLBjammer
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Re: First Attempt - PF Tek [Re: soma_seeker]
    #5733504 - 06/10/06 08:37 AM (17 years, 7 months ago)

Six to seven weeks is a long time to colonize a cake. If the walls of your fruiting chamber are dripping with condensation, your humidity is near 100%. Mist the walls daily with clean water (then fan for 20 seconds or so), and make sure to fan at least 3 times a day to get fresh air in there. High CO2 levels will keep the cake in a vegetative (non-fruiting) state--fresh air is conducive to fruiting. You also need about 12 hours of light per day (full spectrum or indirect sunlight). If you don't have pins in another week, dunk the cake for 24 hours. And definitely get that cake off the perlite (use a lid wrapped in foil or plastic). It's wicking moisture from the perlite and is more prone to contaminate sitting right on the perlite. At this point, that cake is probably overmoistened. I hope my ramblings helped some.


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OfflineSDP
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Re: First Attempt - PF Tek [Re: MLBjammer]
    #5733571 - 06/10/06 09:08 AM (17 years, 7 months ago)

Heeey... im not doubting your knowledge or research capabilities, HOWEVER, just in CASE,  i thought i would direct you here:

http://www.shroomery.org/index.php/par/23805

Just use a 50/50 mix of peatmoss w/ vermiculite. Cakes are great for every beginner! However, you might like the idea of turning several cakes into 1 breadpan casing, then lining the bottom of that there fruiting tupperware with the breadpans (on top of the perlite of course)... The casings provide a good microclimate for pins to form (your RH doesnt have to be as high, around 90%, avoids being "too humid") Just an idea to upgrade a bit after this phase, or now since your havin probs. Thought i would throw it out there for ya!

Btw: Lookin good! Nice roll! Sry its not workin out quite yet. MLBjammer is 100% right... and make sure you fan 3-4 times a day! FAE is a MUST with tubs like that! Good luck!  :thumbup: :crazy2: :tongue2:

-SDP :gethigh:


--------------------
Teonanacatl, open up my eyes
This sacrament, this prayer, beyond the world of lies
Guide me clearly through that which I dont understand
Give me strength to find the path
Help me fight any demons as you flow through me wholely
This is my prayer, that you protect me from evil, and bring me closer to peace
And open up my eyes, so i can see things as you do
Amen


Edited by SDP (06/10/06 09:11 AM)


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OfflineKyleBroflovski
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Registered: 06/05/06
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Re: First Attempt - PF Tek [Re: SDP]
    #5734432 - 06/10/06 02:03 PM (17 years, 7 months ago)

I am new and am getting ready to start my first project so take this with a grain of salt. My first suggestion would be to read all the info you can find on PF.

Also your TiT looks like you used clear tubs. You want solid tubs so light doesn't get in. Light during incubation is bad. You want your jars kept as dark as possible. Also you want to incubate at about 84F. 86F is best but keep in mind that mycelium produces heat as it grows.

Next as far as I can tell you want your FC to be in the low to mid 70's while fruiting. 68F might be a bit on the cold side.
Best thing I can tell you is read more PF teks.
Anyone feel free to correct me on anything if it is wrong. Just trying to help out and more or less regurgitating what I think I've learned. =)


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: First Attempt - PF Tek [Re: KyleBroflovski]
    #5734443 - 06/10/06 02:08 PM (17 years, 7 months ago)

Quote:

KyleBroflovski said:
I am new and am getting ready to start my first project so take this with a grain of salt.
your TiT looks like you used clear tubs. You want solid tubs so light doesn't get in. Light during incubation is bad. You want your jars kept as dark as possible.
Anyone feel free to correct me on anything if it is wrong. Just trying to help out and more or less regurgitating what I think I've learned. =)




I have my jars exposed to light most of the time.
I believe this darkness thing is not very true.
I've found no difference at all between jars colonized in the dark and in the light.


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Offlinesoma_seeker
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Re: First Attempt - PF Tek [Re: monstermitch]
    #5735942 - 06/10/06 10:20 PM (17 years, 7 months ago)

Thanks for all the advice guys.

I've moved the cake onto an aluminium foil covered jar lid and i mist the walls and lid of the terranium every time the condensation is visably decreasing, which is every second day.

As for fanning i was giving it 1 large fanning every day, but since i've added the airstone and a couple of small ventilation holes in the lid (4 days ago) i'm not bothering. The pump seems to supply quite a lot of fresh air, when the lid is open you can clearly here it bubbling away and if you place your hand above the ventilation holes you can feel a very slight pressure of air being pushed out.

I'm aiming for the 'semi-automated PMP' design so that i dont have to fan or mist. Do you think this is possible with my design or do u still recommend misting and fanning? If so, still the same recommended frequency?

Thanks for the link Jr. Mycologist, i have read some similar material. Thanks for the advice on the casings too, but i was hoping to just stick with cakes initially. I'll hopefully be a bit more daring after some initial success.

Cheers


--------------------
Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!


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Offlinesoma_seeker
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Re: First Attempt - PF Tek [Re: soma_seeker]
    #5736109 - 06/10/06 10:52 PM (17 years, 7 months ago)

sorry, i also meant to say that i do keep my incubator covered with a towel to keep the light out and it is held at a constant temp. of 81F (as described as the optimal temp. in the 'PF tek for simple minds').

as for the temp. i am not attempting to alter the terranium's temp. at all, 68F is merely the avg. room temp.

i've read quite a few PF tek pages but some of the info seems to contradict itself and its proving a bit more difficult than i thought to implement, the first cakes i prep'd also were contam'd within 24hrs due to contam'd LC (total noob). having a few initial setbacks but determined to make this work.


--------------------
Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!


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OfflineMLBjammer
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Re: First Attempt - PF Tek [Re: soma_seeker]
    #5737305 - 06/11/06 07:26 AM (17 years, 7 months ago)

I would suggest still fanning and occasionally misting. There's no substitute for hand fanning for fresh air. And keeping your jars exposed to light makes no difference, IME. The casing 101 tek is the best for a beginner, but I think you'll do better with cakes on your first grow. If you want to experiment make a one- or two-cake vermiculite casing, just so you don't put all your eggs in one basket. Vermiculite has no nutrients, so it contams. much less than coir or peat. Coir, by the way, is excellent for double-end casing.
SDP, thanks for the kind comments.


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Offlinesoma_seeker
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Re: First Attempt - PF Tek [Re: MLBjammer]
    #5738479 - 06/11/06 04:00 PM (17 years, 7 months ago)

Thanks for the advice MJB, i will continue to mist and fan. And my terranium receives at least 12hrs indirect light a day from natural and my room's fluorescent light.

was just wondering since i will be misting anyway, do u think it would be beneficial to add say 3% hydrogen peroxide to the sparay bottle as an effort to fight contams?

oh, and yeh thanks a lot for the kind support guys

Cheers


--------------------
Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!


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Offlinecoda
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Re: First Attempt - PF Tek [Re: monstermitch]
    #5738773 - 06/11/06 05:09 PM (17 years, 7 months ago)

Quote:

monstermitch said:
Quote:

KyleBroflovski said:
I am new and am getting ready to start my first project so take this with a grain of salt.
your TiT looks like you used clear tubs. You want solid tubs so light doesn't get in. Light during incubation is bad. You want your jars kept as dark as possible.
Anyone feel free to correct me on anything if it is wrong. Just trying to help out and more or less regurgitating what I think I've learned. =)




I have my jars exposed to light most of the time.
I believe this darkness thing is not very true.
I've found no difference at all between jars colonized in the dark and in the light.




the only problem with exposing your jars to light comes at the end. If you're not careful you can end up with some invitro pinning. I suppose it's not really bad, but it's some wasted energy as the cake can perform better in the FC


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.


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Offlinehyphae
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Re: First Attempt - PF Tek [Re: coda]
    #5738931 - 06/11/06 05:58 PM (17 years, 7 months ago)

Quote:

coda said:
Quote:

monstermitch said:
Quote:

KyleBroflovski said:
I am new and am getting ready to start my first project so take this with a grain of salt.
your TiT looks like you used clear tubs. You want solid tubs so light doesn't get in. Light during incubation is bad. You want your jars kept as dark as possible.
Anyone feel free to correct me on anything if it is wrong. Just trying to help out and more or less regurgitating what I think I've learned. =)




I have my jars exposed to light most of the time.
I believe this darkness thing is not very true.
I've found no difference at all between jars colonized in the dark and in the light.




the only problem with exposing your jars to light comes at the end.  If you're not careful you can end up with some invitro pinning.  I suppose it's not really bad, but it's some wasted energy as the cake can perform better in the FC



I agree this is basic mycology those that don't really understand the process will give that advice. Light initiation is a great trigger when used in coincidence with the other pinning triggers this cannot be over emphasized! This means those who know what their doing initiate all the triggers at the exact same time thus getting the best possible pin initiation. Read my pinning strategy to learn what this process actually consists of. GL :thumbup:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Invisiblemonstermitch
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Re: First Attempt - PF Tek [Re: hyphae]
    #5739116 - 06/11/06 06:49 PM (17 years, 7 months ago)

You guys are both right.
Of course.

I keep a close eye on my jars and don't let them sit in the
incubation room past full colonization.
yes, pins will appear in vitro if I leave the jars in the room colonized.

If some are ahead of the others, I take them from the room and put them in the fridge to wait for the others.

Once all jars are ready, I spawn them and begin the bulk process.

While in trays, I cover them and shield them from light while colonizing.
I don't expose colonizing trays to light, just jars.
once the trays/tubs are ready...

I initiate all pinning triggers at the same time.
Including 14 hours of light per day.

I guess until you get used to it, you can always colonize in the dark.
Just saying it is not mandatory, or all my stuff would have failed.


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Offlinehyphae
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Re: First Attempt - PF Tek [Re: monstermitch]
    #5739350 - 06/11/06 07:49 PM (17 years, 7 months ago)

What you suggest monstermitch will work just fine also. :wink:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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