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zeegos
Shroomagator


Registered: 04/03/06
Posts: 827
Loc: bat country
Last seen: 3 years, 24 days
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mycelium has halted
#5731199 - 06/09/06 03:41 PM (17 years, 7 months ago) |
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Well its week 3 today and the jar is about 2/3 colonised. I have noticed over the past 2-3 days that there havent been any advances in the colonisation of the mycelium. Its getting plenty air exchange, its at the right temp and its in a dark incubator. The only reason i can think of is because once i thought i had cobweb in the jar which i then attacked with 17.5% H2O2 which burnt it away at that section but then someone told me it wouldent have been cobweb. Will it continue to grow? Today i decomposed some H2O2 in a syringe and injected 10ml of pure oxygen to encourage growth.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: mycelium has halted [Re: zeegos]
#5731255 - 06/09/06 03:57 PM (17 years, 7 months ago) |
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Where is the 1/3 uncolonized substrate top, bottom? What size jars and what type substrate? Sounds to me possibly a water content issue. What doest yopur gas exchange consist of?
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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zeegos
Shroomagator


Registered: 04/03/06
Posts: 827
Loc: bat country
Last seen: 3 years, 24 days
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Re: mycelium has halted [Re: hyphae]
#5731268 - 06/09/06 04:01 PM (17 years, 7 months ago) |
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the mycelium has colonised evenly in the cake (top and bottom), it isnt a CO2 densiy issue. I am using a 1/2 pint glass with tinfoil as a lid which has 4 open holes in it. I have flipped it over a week ago which helped the bottom to colonise a bit. Its just these past few days that i have noticed to further growth.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: mycelium has halted [Re: zeegos]
#5731298 - 06/09/06 04:12 PM (17 years, 7 months ago) |
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With a foil lid and no filter, there's a very good chance it has become contaminated with bacteria. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Tweexican
Clit Commander

Registered: 11/06/03
Posts: 657
Last seen: 5 years, 6 months
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Re: mycelium has halted [Re: RogerRabbit]
#5731311 - 06/09/06 04:15 PM (17 years, 7 months ago) |
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yeah bacterial contamination can take hold so much faster than mycelium.
For this reason mycelium will halt its "growing" mode and start fighting off the infection.
--------------------
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zeegos
Shroomagator


Registered: 04/03/06
Posts: 827
Loc: bat country
Last seen: 3 years, 24 days
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Re: mycelium has halted [Re: Tweexican]
#5731347 - 06/09/06 04:28 PM (17 years, 7 months ago) |
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sheisse Im gonna chop off the uncolonised part, soak in h2o2 water and spawn to hpoo good idea?
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 8 months
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Re: mycelium has halted [Re: zeegos]
#5731599 - 06/09/06 06:11 PM (17 years, 7 months ago) |
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That will work.
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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zeegos
Shroomagator


Registered: 04/03/06
Posts: 827
Loc: bat country
Last seen: 3 years, 24 days
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Re: mycelium has halted [Re: hyphae]
#5733776 - 06/10/06 10:44 AM (17 years, 7 months ago) |
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oh wait, there she goes  Close call
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