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Offlinezeegos
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mycelium has halted
    #5731199 - 06/09/06 03:41 PM (17 years, 7 months ago)

Well its week 3 today and the jar is about 2/3 colonised. I have noticed over the past 2-3 days that there havent been any advances in the colonisation of the mycelium. Its getting plenty air exchange, its at the right temp and its in a dark incubator.
The only reason i can think of is because once i thought i had cobweb in the jar which i then attacked with 17.5% H2O2 which burnt it away at that section but then someone told me it wouldent have been cobweb.
Will it continue to grow?
Today i decomposed some H2O2 in a syringe and injected 10ml of pure oxygen to encourage growth.


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Offlinehyphae
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Re: mycelium has halted [Re: zeegos]
    #5731255 - 06/09/06 03:57 PM (17 years, 7 months ago)

Where is the 1/3 uncolonized substrate top, bottom? What size jars and what type substrate? Sounds to me possibly a water content issue. What doest yopur gas exchange consist of?


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Getting the most out of your casings!, A pinning strategy.
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Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinezeegos
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Re: mycelium has halted [Re: hyphae]
    #5731268 - 06/09/06 04:01 PM (17 years, 7 months ago)

the mycelium has colonised evenly in the cake (top and bottom), it isnt a CO2 densiy issue. I am using a 1/2 pint glass with tinfoil as a lid which has 4 open holes in it. I have flipped it over a week ago which helped the bottom to colonise a bit. Its just these past few days that i have noticed to further growth.


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OfflineRogerRabbitM
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Re: mycelium has halted [Re: zeegos]
    #5731298 - 06/09/06 04:12 PM (17 years, 7 months ago)

With a foil lid and no filter, there's a very good chance it has become contaminated with bacteria.
RR


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OfflineTweexican
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Re: mycelium has halted [Re: RogerRabbit]
    #5731311 - 06/09/06 04:15 PM (17 years, 7 months ago)

yeah bacterial contamination can take hold so much faster than mycelium.

For this reason mycelium will halt its "growing" mode and start fighting off the infection.


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Offlinezeegos
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Re: mycelium has halted [Re: Tweexican]
    #5731347 - 06/09/06 04:28 PM (17 years, 7 months ago)

sheisse
Im gonna chop off the uncolonised part, soak in h2o2 water and spawn to hpoo
good idea?


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Offlinehyphae
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Re: mycelium has halted [Re: zeegos]
    #5731599 - 06/09/06 06:11 PM (17 years, 7 months ago)

That will work. :wink:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinezeegos
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Registered: 04/03/06
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Re: mycelium has halted [Re: hyphae]
    #5733776 - 06/10/06 10:44 AM (17 years, 7 months ago)

oh wait, there she goes  :laugh:
Close call  :crazy:


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