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soma_seeker
Stranger in aStrange Land


Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 3 months, 20 days
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LC contaminated?
#5729514 - 06/09/06 03:10 AM (17 years, 7 months ago) |
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this is my first attempt at growing and yesterday i inoculated two sets of four half pint jars; 4 with 'KSSS' and 4 with 'maxi-pal' (apologies for the ignorance with naming).
the KSSS jars i prep'd several days before sterilizing (steam-sterilized in a large pot), whereas the maxi-pal jars i prep'd, then immediatly sterilized the night before innoculating.
anyway when i checked my incubator this evening all four of the KSSS were stained bright pink with contam that quite obviously only covered the area touched by the injected LC.
my question is do you think it is likely that the LC is contaminated (and not just the jars) as i want to use it when i prep the jars again??
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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Thai_connection
Siam


Registered: 10/01/05
Posts: 247
Loc: Thailand
Last seen: 13 years, 3 months
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most likely the lc, since you said it follows the injection sites.
peace siam
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RemainRandom50
Do You Need ToKnow Me?
Registered: 01/15/06
Posts: 1,695
Last seen: 14 years, 9 months
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steam sterilization via no pc.....always a problem IMO and from expierence. i would targe that first.
-------------------- At times I get consumed by my everyday life and will leave the Shroomery. Yet, every time drugs come falling into my life for fun.....I always think about the Shroomery and then I'm back!
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soma_seeker
Stranger in aStrange Land


Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 3 months, 20 days
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cheers guys. i prep'd and sterilized the jars again last night and inoculated again this morning. i inoculated one with the troublesome LC to see how it goes. as for the PC, i tried to source one but were too expensive, thought i'd give this method a good try first.
is it common for jars to become very obviously contam'd in only 24hrs?
Thanks
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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Thai_connection
Siam


Registered: 10/01/05
Posts: 247
Loc: Thailand
Last seen: 13 years, 3 months
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let us know...also if you go to the thrift stores or goodwill stores they may have some pc cheap! peace siam
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soma_seeker
Stranger in aStrange Land


Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 3 months, 20 days
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yeh i will and yeh i tried a few goodwill stores and no luck, did manage to get a really good tall pot with a tight fitting lid though so it was a decent consilation.
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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Thai_connection
Siam


Registered: 10/01/05
Posts: 247
Loc: Thailand
Last seen: 13 years, 3 months
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some use fractional sterlization, comes out good as pc'ing but takes a bit time. still think its the lc
peace siam
Edited by Thai_connection (06/10/06 12:51 AM)
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soma_seeker
Stranger in aStrange Land


Registered: 04/02/06
Posts: 367
Loc: Down under
Last seen: 3 months, 20 days
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you were right it was the LC. Only 12 hrs after inoculation and it showed up in the repeated jar again. Oh well, making another LC of that strain now as i'm still keen to try and grow both at once.
Cheers for the help
-------------------- Taking a psychedelic is analogous to life, if you dwell on reaching the end you'll never enjoy the trip!
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