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OfflineDucky
Governor ofRhythm
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Registered: 05/09/06
Posts: 71
Loc: on a boat somewhere
Last seen: 17 years, 5 months
Playing with Agar
    #5728690 - 06/08/06 09:59 PM (17 years, 7 months ago)

A friend made up an agar substitute and put a piece of mycellium approx the diameter of a quarter of a pencil eraser head in the middle of each two nights ago. Tonight, having kept them at room temperature the whole time, they are both approx one centimeter across, and bright white! No sterility precautions after cooking it all up were taken, except incorporating H2O2 into the mixture.

The recipe goes:
2 packets unflavored jelatin
1 tbsp bee pollen kernels
slightly less than one cup of buckwheat flour
40ml H2O2
enough water to make the mixture about 1 liter

It was mixed together with hot water to ensure that everything got nice and melted and gooped up, and then actually the H2O2 was added around 110 deg.F. The cultures are growing in non-sterilized containers of various sorts, mainly what was available.

Also, a little clump of perlite with a few little knots which had just formed (taken from another pf style cake) were added to their own bit of the buckwheat jello, and have now turned brown on their tips and are continuing to grow.

Has anyone heard of or tried doing a similar agar sub?


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OfflineBabo911
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Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
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Re: Playing with Agar [Re: Ducky]
    #5728829 - 06/08/06 10:32 PM (17 years, 7 months ago)

I don't think that you could concider this agar with out actually adding agar hehe. Agar is an extract of sea weed if I am not mistaken.

It seems like the myc is eating through the knox jellotin or what ever you used, I know jellotin is primarly made of bone so that might be somehitng to look into.

One last thing.... I have never heard of myc growing on perlite.... you mean verm?


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Invisiblecreamcorn
mad scientist
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Registered: 03/13/06
Posts: 2,962
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Re: Playing with Agar [Re: Babo911]
    #5728881 - 06/08/06 10:50 PM (17 years, 7 months ago)

yeah, gelatin and pectin are cheap agar alternatives when sterility isnt of concern and are used sometimes in certain lab situations. they do the same thing as agar, but agar holds up to the higher temps of PC'ing, whereas had you PC'd your gelatin it wouldn't have gelled up, as you would have destroyed the protiens.


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OfflineDucky
Governor ofRhythm
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Registered: 05/09/06
Posts: 71
Loc: on a boat somewhere
Last seen: 17 years, 5 months
Re: Playing with Agar [Re: creamcorn]
    #5728936 - 06/08/06 11:06 PM (17 years, 7 months ago)

interesting about the proteins..makes sense. they hardly can hold up in this summer weather!

ya..perlite. I know, it's weird, but a mini cake used for an experimental solitary grow chamber has a few pins and other, bigger guys growing not only off of it, but also from the surrounding perlite, yes perlite. vermiculite was used in the cake tho yes.


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Offlineguest1
Mycena
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Registered: 05/25/09
Posts: 852
Last seen: 1 year, 2 months
Re: Playing with Agar. - Ans: (jello doesn't work) [Re: Ducky]
    #10609860 - 07/02/09 01:30 PM (14 years, 6 months ago)

"The gelatin is consumed by the mycelium, turning the jello back to liquid.  It's been done hundreds of times already with total failure.  It doesn't work.  In addition, it gets really stinky.
RR "

http://www.shroomery.org/forums/showflat.php/Number/10113151#10113151

Brown Rice Flour (Agar Alternative)
http://www.shroomery.org/8514/Agar-substitute

I thought this thread should be resolved instead of forever a dead end that leaves people to more searching for the answer about using jello instead of agar.


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Edited by guest1 (07/02/09 01:37 PM)


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OfflineElderlyone
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I'm a teapot User Gallery

Registered: 06/08/09
Posts: 222
Last seen: 6 months, 15 days
Re: Playing with Agar. - Ans: (jello doesn't work) [Re: guest1]
    #10610028 - 07/02/09 01:58 PM (14 years, 6 months ago)

bumb guest


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