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Invisibleliamtheloser
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Registered: 06/07/06
Posts: 1,453
A few experiments
    #5725173 - 06/07/06 11:40 PM (17 years, 7 months ago)

Ok, so I'm fairly new to the scene, but I've got my first few successful batches done. But now I've started to experiment... Both with substrates and LCs. I just wanted to share my experiments with everyone, because i thought i got fairly interesting results.

first, my experimental substrate, not innoc'd but soon:

I cooked up 1 cup of whole grain organic white rice along with 1/4 cup of whole grain oatmeal (not quick oats), and added 1/4 cup of brown sugar (i know, high contam chance, but hey, its an experiment).

At the time I cooked this all up, my PC was out of commission (corroded seal) so I decided to use a sort of tub in tub microwave method. I strained and placed the substrate into a 1/2qt ziplock screwtite container and put that into a pyrex dish full of water which nearly enveloped the ziplock container (water was about 1/4 inch from the lid). the lid had 4 small holes just like a glass jar. I microwaved this setup for 10 minutes, and the rice stayed moist, not overcooked, no sign of burning. It's been almost 2 weeks, i haven't innoced yet due to fear of contam. but i've kept it in my growth chamber at 86*f and there isn't any signs of contam... Should I innoc? or give more time to check for contams.

LC: The juice from the rice/oatmeal/brown sugar was collected and placed in a zip-lock bag, which i innoc'd with 2cc's of mazaptec spores, i placed it in my growth chamb 2 days ago, but no signs of myc yet. no signs of contams either though... anyone think this will work?



my incubation/growth chamber is a 32 litre black rubbermaid container. It's just sitting on a heating pad, and if i keep a towel over my jars, the whole thing stays at exactally 86 degrees fahrenheit.


any comments/suggestions would be cool... and i love this place, this is my first post, but i've read all the faq's and teks, and i've probably ready every post for the last 3 months or so... i love everyone here and hope i have a long and fruitful (no pun intended) relationship with everyone here! thanks for all the help.

-- liam


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Invisibleliamtheloser
Advanced Idiot
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Registered: 06/07/06
Posts: 1,453
Re: A few experiments [Re: liamtheloser]
    #5725193 - 06/07/06 11:48 PM (17 years, 7 months ago)

Ok, i just realized i hadn't checked my LC today, and when i checked, there was a huge cloud of myc in it. HUGE, so i just shook up my ziplock bag and i guess we'll see what happens in the next few days!


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Offlinemycofiliac
Known before asSpaceman
Male
Registered: 05/31/06
Posts: 77
Loc: Children of the corn
Last seen: 16 years, 10 months
Re: A few experiments [Re: liamtheloser]
    #5725230 - 06/07/06 11:59 PM (17 years, 7 months ago)

Good luck, it's good to see people doing research and trying things out for the first time. Please let me know what becomes of it.

See you around
Space


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Swimming in the environment


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