Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineTinkal
Green Thumb
 User Gallery

Folding@home Statistics
Registered: 06/01/05
Posts: 257
Loc: Europe
Last seen: 2 years, 8 months
Liquid Culture w/ honey - Malabar
    #5722081 - 06/07/06 06:39 AM (17 years, 7 months ago)

This is a pictorial from my first LC w/ honey that I started with a friend a month ago. We used a small part of a Malabar print.



Used used Dettol (Chloroxylenon) for sterilizing the razor and other stuff.



These are the tools used for LC :

1. Sterile water
2. Wild honey
3. Jar
4. Steam Pot



The lid of the jar with a hole made with nail.



The honey only in the jar. After every action we always closed the jar with the first aluminum foil.



This is after the mixture with the sterile water.



We boiled the jar for about 45' and let it cool. After that we scraped spores from a print and covered the jar with the second aluminum foil.



The jar after 2 days.



The jar after 7 days.

After the seventh day I put the jar in the fridge.

I just wanted to ask, since it's the first time I do this process, are the cloud that is visible in the jar, sight of growth, or something else.
I have seen pictures in the past, that are circular white structures inside the liquid.


Edited by Tinkal (06/09/06 04:03 PM)


Extras: Filter Print Post Top
Offlinetallgreen
chillin like avillain
 User Gallery
Registered: 05/21/06
Posts: 293
Last seen: 17 years, 4 months
Re: Liquid Culture w/ honey - Malabar [Re: Tinkal]
    #5723350 - 06/07/06 03:59 PM (17 years, 7 months ago)

Today I'm also doing my first LC with honey and Malabar(syringe).  :cheers:

I'll be inoculating tonight.  I'll let you know how it goes.

I have heard that cloudiness indicates bacterial growth, but yours looks like that right after sterilization right?  I can see little chunks in your photo that look like mycelium.  Sorry, I'm not help I guess?


--------------------
Nothing you can know that isn't known.
Nothing you can see that isn't shown.
Nowhere you can be that isn't where you're meant to be.
It's easy.
All you need is love.
- The Beatles


Extras: Filter Print Post Top
OfflineFeelers
Anti-Myth-Rhythm-Rock-Shocker
Male User Gallery

Registered: 06/18/02
Posts: 1,806
Loc: Land of Oz
Last seen: 5 years, 8 months
Re: Liquid Culture w/ honey - Malabar [Re: tallgreen]
    #5723999 - 06/07/06 07:08 PM (17 years, 7 months ago)

Hey man from what Ive read you are only supposed to pressure cook for 15 mins MAX. Otherwise this caramelizes the sugars which cause mutations and screw up your culture.
15 mins is plenty for liquid to sterilise.

Also did you use the clear type honey? - I cant tell but make sure you use the light "treacle" type stuff.


Extras: Filter Print Post Top
OfflineAscension
Stranger

Registered: 01/13/05
Posts: 156
Loc: Still trying to work it o...
Last seen: 17 years, 7 months
Re: Liquid Culture w/ honey - Malabar [Re: Feelers]
    #5725890 - 06/08/06 06:54 AM (17 years, 7 months ago)

It sounds like you scraped the spores into the jar, THEN boiled it for 45 mins?

I hope you didnt!

Also this will prob be moved soon, but just letting you know for when it disappears, its been posted in the wrong forum and you'll be able to find it in the mush cultivation when its moved.

Cheers, hope it turns out okai. I stuffed up my first 3 LCs, but once you get it right, it makes everything so much more easier.


Extras: Filter Print Post Top
OfflineFeelers
Anti-Myth-Rhythm-Rock-Shocker
Male User Gallery

Registered: 06/18/02
Posts: 1,806
Loc: Land of Oz
Last seen: 5 years, 8 months
Re: Liquid Culture w/ honey - Malabar [Re: Ascension]
    #5727751 - 06/08/06 06:00 PM (17 years, 7 months ago)

I missed that!

Tinkal by putting the spores in first you end up sterilizing them too - they will all be dead.

Shit happens. :laugh:


Extras: Filter Print Post Top
OfflineTinkal
Green Thumb
 User Gallery

Folding@home Statistics
Registered: 06/01/05
Posts: 257
Loc: Europe
Last seen: 2 years, 8 months
Re: Liquid Culture w/ honey - Malabar [Re: Feelers]
    #5731407 - 06/09/06 04:58 PM (17 years, 7 months ago)

Sorry my fault. I didn't killed the spores. First I boiled the water and after cooling the jar I scraped the spores.

:smile:


--------------------
Ask me about my seed collection and trading!

"Of all the things I love the most, my mind is what I miss the most"


Extras: Filter Print Post Top
OfflineINFECTEDGOAT420
Stranger
Registered: 06/07/06
Posts: 6
Last seen: 17 years, 7 months
Re: Liquid Culture w/ honey - Malabar [Re: Tinkal]
    #5732625 - 06/09/06 11:21 PM (17 years, 7 months ago)

I am using a syringe. I need to know how many cc's of spores i need to inject in the culture.


Extras: Filter Print Post Top
Offlinekilroy
Hightimes
Male

Folding@home Statistics
Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit Flag
Last seen: 13 years, 3 months
Re: Liquid Culture w/ honey - Malabar [Re: INFECTEDGOAT420]
    #5732642 - 06/09/06 11:28 PM (17 years, 7 months ago)

okay lets clear some of this up 15 min max if you pc it, steaming you have to go longer. Infectedgoat 1 to 2cc is enough to innoc an LC. Tinkal did you pc or steam it, I ask this because that is the strangest pot I have seen.


--------------------
IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





Extras: Filter Print Post Top
OfflineTinkal
Green Thumb
 User Gallery

Folding@home Statistics
Registered: 06/01/05
Posts: 257
Loc: Europe
Last seen: 2 years, 8 months
Re: Liquid Culture w/ honey - Malabar [Re: kilroy]
    #5733251 - 06/10/06 04:35 AM (17 years, 7 months ago)

I steamed it kilroy. By the way, why it's the strangest pot ?

I've seen other LCs in shroomery :
http://www.shroomery.org/forums/files/05-41/906040667-pclc7.jpg

Mine is not so cloudy. What might happened? I saw white clouds but they were not so many like other cultures. Can I put the jar out of the fridge so the mycelium will multiply more ?


--------------------
Ask me about my seed collection and trading!

"Of all the things I love the most, my mind is what I miss the most"


Edited by Tinkal (06/10/06 04:40 AM)


Extras: Filter Print Post Top
OfflinePsiloman
member
Male
Registered: 04/11/03
Posts: 1,116
Loc: Europe
Last seen: 10 years, 4 months
Re: Liquid Culture w/ honey - Malabar [Re: Feelers]
    #5733674 - 06/10/06 10:07 AM (17 years, 7 months ago)

Hmmm, could one use glucose in the place of honey?

It can be found easily and its clear.

Tinkal incubate some more in a warm dark place and take again a photo of it.


Extras: Filter Print Post Top
Offlinekilroy
Hightimes
Male

Folding@home Statistics
Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit Flag
Last seen: 13 years, 3 months
Re: Liquid Culture w/ honey - Malabar [Re: Psiloman]
    #5733818 - 06/10/06 11:01 AM (17 years, 7 months ago)

Tinkal: Yes you can pull it out and it will start agn. you only put in frigde to stall its growth.
Psiloman: I do not beleive glucose is a good idea but you can do a search to find out, if you want clear then use Karo or any lite corn syrup with vanilla in it, most of them have it already in it.
GL u 2


--------------------
IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Pan cyan in petri dish plus giant pan & liquid culture memberjockey 1,508 4 03/02/18 10:09 AM
by meltdowner
* Liquid Culture injection problem Bloomer69 2,017 5 03/14/03 01:48 AM
by BikeCourier
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 52,763 43 07/08/23 07:04 AM
by Screwup
* Liquid Culture Challenges Leimana 9,559 4 05/31/05 11:33 PM
by Corporal Kielbasa
* 1st Liquid Culture, arrrgh! Seril 14,293 10 02/28/06 08:35 PM
by Limelight_Liver
* Liquid Culture Recipes MrMaddHatter 23,904 13 03/29/05 04:53 AM
by Sbealzebub
* how to use cardboard cultures to inoculate grain jars and how to use a liquid culture to inoculate lordoftheshroomz 6,509 12 05/08/05 03:55 PM
by lordoftheshroomz
* Clear liquid cultures ? mickywilliams2 3,665 11 10/12/05 05:02 PM
by Bamaman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,472 topic views. 28 members, 192 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.006 seconds on 12 queries.