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Offlineshroomballa
ShroomBalla
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Registered: 03/26/06
Posts: 263
Loc: Montana
Last seen: 16 years, 6 months
Ahhhh! Invitro pin w/o full popcorn colonization! *PIC*
    #5721105 - 06/06/06 10:48 PM (17 years, 7 months ago)

A popcorn jar is ~70% colonized. It's been in the dark, away from FAE, with a polyfil lid. When examined today (it is every 3 days) I noticed a 1" pin!!!! shittt, what do I do? It's going to be spawned to hpoo, but when should I do it?
I'm under the impression that once pinning has been triggered, colonization stops. Is this correct? Is it hosed?
Also, the pin has a really dark cap, so i'm assuming it's an abort, but it's pretty tall for an abort.
First pic: pin

Second pic: back side

It's been colonizing for 5 weeks (this B+ has been a really slow colonizer. usually 4-5 weeks for a 1/2 pt BRF jar)


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Anything I say is fictional. Duh.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: shroomballa]
    #5721117 - 06/06/06 10:53 PM (17 years, 7 months ago)

just empty the jar and spawn it.

take out the pin and eat it.

you're fine.


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: monstermitch]
    #5721267 - 06/06/06 11:28 PM (17 years, 7 months ago)

Quote:

monstermitch said:
just empty the jar and spawn it.

take out the pin and eat it.

you're fine.



That's bullshit mitch. Why do you keep telling people all the wrong stuff in every damn thread? You don't know what the hell you're talking about so quit pretending.

Friend, that jar looks like it has wetspot bacterial contamination. That's the reason it pinned when it did. The survival mechanism of the fungi sensed danger and the natural reaction is to reproduce. That's why it fruited when it did. Even the parts of the popcorn that have mycelium, have a very weak looking growth. The greasy appearance of the grains, especially where they touch the glass give away bacillus.

Here's what a popcorn jar of mycelium should look like.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: RogerRabbit]
    #5721289 - 06/06/06 11:35 PM (17 years, 7 months ago)

my bad.

didn't even look at the pictures.
should know better.

you are correct as always.

and an asshole as usual.

guess you don't like me huh?
like I fucking care.

thanks for such a great attitude, appreciate it.


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Offlinemajlo
crazy
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Registered: 04/20/06
Posts: 168
Last seen: 14 years, 10 months
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: monstermitch]
    #5721926 - 06/07/06 03:44 AM (17 years, 7 months ago)

just listen to RR and you're on safe side :smile:

Keep it that way RR!


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Come see our country, but don't stay too long :smile:


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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: majlo]
    #5722131 - 06/07/06 07:31 AM (17 years, 7 months ago)

still a jerk eh rodger ?
lol
guess that's why
no one misses you
when you go away.
in all your puffed up strutting to show off
your vast knowledge
and humble poor mitch
you neglected to give
shroomballa any useful advice,
typical.

shroomballa
i'd dump your pcorn out in a tray and let it set 48 hours to
recover,
after that discard any uncolonized pcorn
and case the good stuff remaining.


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InvisibleArrakis
PsilocybinPsycho

Registered: 03/03/04
Posts: 276
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: Hippie3]
    #5722157 - 06/07/06 07:53 AM (17 years, 7 months ago)

I miss him; Roger Rabbit was actually my imaginary friend when I was 4.  :sad:


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Funniest shit I've read in a while:
http://www.shroomery.org/forums/showflat.php?Cat=&Number=886829&page=&view=&sb=5&o=&fpart

"He who controls the spice controls the universe!"


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Offlineshroomballa
ShroomBalla
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Registered: 03/26/06
Posts: 263
Loc: Montana
Last seen: 16 years, 6 months
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: shroomballa]
    #5722159 - 06/07/06 07:54 AM (17 years, 7 months ago)

So I soaked or simmered too long then..?


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Anything I say is fictional. Duh.


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Offlinemajlo
crazy
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Registered: 04/20/06
Posts: 168
Last seen: 14 years, 10 months
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: Arrakis]
    #5722161 - 06/07/06 07:55 AM (17 years, 7 months ago)

why is RR now the one to blame now hippe3 ?
anything i missed? or is it just you?

what if that is really a contam (i am not sure because i am not an expert)


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Come see our country, but don't stay too long :smile:


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OfflineChromeCrow
one ancient mutha
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Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: majlo]
    #5722210 - 06/07/06 08:38 AM (17 years, 7 months ago)

I can see where RR is coming from, it gets so frustrating continually fixing the bad advice that others give...

but after looking closely at the pic, then even blowing it up a bit in Photoshop, i have to disagree about this being a bacterial contam, and if it is, its at the first stages.

there is no tell tale sign of bacteria.

what i did notice tho, is the corn doesnt seem fully hydrated.. its hardley swollen at all .. it looks at most 50% to its max level..

its hard to get popcorn fully hydrated with out at least a minor bursting /crack in the corns shell.... i see NONE on the uncolonized corn


my advice is to give it a few days.. if it is bacterial it will definately show itself .. keep it isolated of course


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ISO: Orissa, Malabar, z strain


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Offlinesa_mull
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Registered: 05/24/06
Posts: 144
Loc: IO
Last seen: 6 years, 3 days
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: ChromeCrow]
    #5722232 - 06/07/06 08:57 AM (17 years, 7 months ago)

I know i'm new to this forum, but Rogers has already helped me out a few times. i appreiate it. don'tlisten to them Roger. I enjoy your knowledge you left through the threads. I think they are jsut jelous, that's why they attacked you. Keep the peace and wisdom


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OfflineEquilibriuM
dream stalker

Registered: 07/17/05
Posts: 2,323
Last seen: 16 years, 7 months
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: sa_mull]
    #5722236 - 06/07/06 09:01 AM (17 years, 7 months ago)

I did think that was kind of rude but I don't know the whole story.

I also think that wetspot is a good diagnosis.


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HELP!!!!!!!!!


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OfflineRogerRabbitM
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Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: ChromeCrow]
    #5722242 - 06/07/06 09:05 AM (17 years, 7 months ago)

The greasy appearance of the kernels is what indicates wetspot. I never recommend trying to 'save' a contaminated jar. That leads to more problems later. The jars aren't dry, or the invitro fruit couldn't have formed. A slightly dry popcorn jar will actually colonize faster than a wet one. A common mistake is to think that wetspot contamination is, or is caused by, wet jars.

I jumped poor mitch's ass because nobody can keep up with his post whoring bad advice in every thread. He's on his first grow and has never produced a crop, a grow log, or a single picture of a grow. His 1000+ posts in less than four months are almost entirely posting totally bad advice by misinterpreting what he reads in other people's posts. Pull up his posts and read them, starting from the first. You'll see the steady progression from asking noob questions, to answering with authority like someone with actual experience. Now open his journal and see all the 'monstermitch's teks'. They are cut and pasted from mine and other experienced growers posts, then he puts his name on them and calls them his own. That is plagiarism, plain and simple.

In several years on all the boards, this is the first time I've ever called anyone down for bullshitting. Well, that is except for you hippie3. I suppose that makes twice now. I apologize if I've offended anyone.
edited because I spelled plagiarism wrong the first time.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Edited by RogerRabbit (06/07/06 09:17 AM)


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: RogerRabbit]
    #5722334 - 06/07/06 10:09 AM (17 years, 7 months ago)

:hitler:

pardon me for just getting a camera.
didn't know pictures were a requirement.
first grow?  not even close.
did I know less when I came here?  Of course.

sorry, can't be god like you.

p.s.  my advice was very poor.
wether it is bacteria or not... do not spawn that jar.
despite what anyone else may say, I personally go with
RR's assesment.  Though right now I'd rather not.

I would toss that jar.
no reason to listen to me though.


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Offlinehyphae
born to grow
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Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: ChromeCrow]
    #5722561 - 06/07/06 11:40 AM (17 years, 7 months ago)

Quote:

ChromeCrow said:
I can see where RR is coming from, it gets so frustrating continually fixing the bad advice that others give...

but after looking closely at the pic, then even blowing it up a bit in Photoshop, i have to disagree about this being a bacterial contam, and if it is, its at the first stages.

there is no tell tale sign of bacteria.

what i did notice tho, is the corn doesnt seem fully hydrated.. its hardley swollen at all .. it looks at most 50% to its max level..

its hard to get popcorn fully hydrated with out at least a minor bursting /crack in the corns shell.... i see NONE on the uncolonized corn


my advice is to give it a few days.. if it is bacterial it will definately show itself .. keep it isolated of course




I have a tendency to agree here, I myself do not see any wetspot but time will tell. Personally I'd shake it and if it recovers there was never any infection and by shaking full colonization will easily occur if of course moisture content of the grain is right. GL


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Ahhhh! Invitro pin w/o full popcorn colonization! *PIC* [Re: RogerRabbit]
    #5726011 - 06/08/06 08:27 AM (17 years, 7 months ago)

Quote:

RogerRabbit said:In several years on all the boards, this is the first time I've ever called anyone down for bullshitting...
RR





liar.
:thumbdown:


--------------------
Admin @ mycotopia.net
Mycotopia


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