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OfflineShaqAttack
JarHead

Registered: 06/18/04
Posts: 187
Loc: Armpit of the North East
Last seen: 9 years, 8 months
LC Question?
    #5720900 - 06/06/06 09:52 PM (17 years, 7 months ago)

Hi - How's it going? I like to do my LC in sterile multi cc vials. I've read posts where air exchange is not vital for LC and I've also read where air exchange is helpful. My question is if I leave the vent needle in the rubber stopper and bottle above the liquid line will or how does it the whole shabang stay sterile? Would the needle or does the needle "wick" contams in any way? And does polyfill keep out bacteria?



Thanks
Shaq


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: LC Question? [Re: ShaqAttack]
    #5720912 - 06/06/06 09:55 PM (17 years, 7 months ago)

it stays sterile with the poly-fil filter.

keep the poly-fil dry and you are good.

don't leave the needle in for any longer than a week at most.

the self-healing port will then be compromised.


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OfflineShaqAttack
JarHead

Registered: 06/18/04
Posts: 187
Loc: Armpit of the North East
Last seen: 9 years, 8 months
Re: LC Question? [Re: monstermitch]
    #5720963 - 06/06/06 10:08 PM (17 years, 7 months ago)

Cool - Thanks MM!!! :thumbup:


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Invisiblemonstermitch
Growing in Bags Doesn't Work

Folding@home Statistics
Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay Flag
Re: LC Question? [Re: ShaqAttack]
    #5721085 - 06/06/06 10:42 PM (17 years, 7 months ago)

sure.

hope all turns out well.


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