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Offlineetam
it's a hobby...
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Karo LC - Carmelization problem.
    #5717892 - 06/06/06 03:14 AM (17 years, 7 months ago)

I've been attempting to use Agar's baby bottle LC tek. It seems so fool proof I'm really feeling a bit dumb with this.

I've been PC'ing the baby bottles containing 200ml water and 6ml light karo at 15 PSI for 15 minutes.

Every single time without fail the solution has carmelized and turned a brownish color.

What can I do besides shortening the PC time to prevent this from happening?


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Invisiblecreamcorn
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Re: Karo LC - Carmelization problem. [Re: etam]
    #5718499 - 06/06/06 10:53 AM (17 years, 7 months ago)

well there's gonna be people that disagree with me but i still say the bit of yellowing isn't carmelization at all, it doesn't make sense that it is from a chemistry point of view, and i've never had problems with growth in a yellowed solution. i even kind of like the yellowing because they seem to go clear as the mycelium grows, giving a hint you might soon be running low on nutes and its time to store in the fridge or whatnot...

that aside, you don't have to PC LC. i used to, but after steam sterilizing many and having 0% contam rates doing so, i don't bother (my PC's valve sticks and makes me paranoid i'm gonna blow the place up, so i avoid it when i can!) you do have to steam sterilize longer - 30 to 45 minutes, i'd go closer to the 45 minute point to be safe with 200ml, but 30 mins never failed me on half pints with 100ml of solution... you'll get a perfectly clear karo LC that way.


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InvisibleAtheist
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Registered: 01/24/06
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Re: Karo LC - Carmelization problem. [Re: creamcorn]
    #5718517 - 06/06/06 11:01 AM (17 years, 7 months ago)

i put 3ccs in 100ml doing the same baby bottle tek

my bottles were half the size

and I PCed at 15psi for 20 minutes

they were crystal clear

no color at all


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