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 frogsheath
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 Stalled jars - NO MORE! UPDATE 
#571774 - 03/06/02 05:48 PM (23 years, 1 month ago) |
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Originally my problem was stalled jars. They were inoculated on 1-29
and were a little more than 1/2 colonized at best. I asked for
suggestions for salvaging or expediting the colonization process.
You all responded with great tips. I also had recently lost 12 other
jars (inoculated w/ Mazatec on 2-21) due to some kind of yellow-tinted
bacteria that settled in before spores germinated (I thought).
I inoculated 10 more jars w/ Mazatec on 1-2 and feared I was starting
to see the awful yellow tint taking place again! My syringes were kind
of old (received 'em by mail in november). I thought that may have
been a potential cause. Go to the end of this thread for new pics
of the situation. I will put them up right now. Thanks.
Peace.
Edited by frogsheath (03/20/02 12:39 PM)
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 Hippie3
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 Re: Stalled jars [Re: frogsheath]
#572274 - 03/07/02 05:58 AM (23 years, 1 month ago) |
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the ones done 1/29 are most likely contam'd or they'd be finished by now.
are you using a pressure cooker to sterilize ?
syringes from november should still be fine,
assuming they were good to begin with.
--------------------
Admin @ mycotopia.net
Mycotopia
Edited by Hippie3 (03/07/02 05:59 AM)
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frogsheath
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 Re: Stalled jars [Re: Hippie3]
#572330 - 03/07/02 08:42 AM (23 years, 1 month ago) |
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No sir (or Ma'am). With a large fairly tight-lidded steamer/kettle (Martha Stewart/K-Mart brand) substrate was steamed w/lids loosened at the lowest possible boil for an hour and some. The jars were individually covered w/ tin foil to fend off water seepage. 3 jars were steamed at a time. That number was raised to 6 after the realization that jars could be stacked in the kettle. This could be a problem me thinks. I don't like it. It seems all my jars have (and are) colonizing exceedingly slow. Thanks for the reply Hippy3. Then my last 2 syringes (Hawaiian) should be good? Time for a change in tek.
Peace.
Edited by frogsheath (03/08/02 03:41 AM)
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BeppoMarx
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Re: Stalled jars [Re: frogsheath]
#572335 - 03/07/02 08:53 AM (23 years, 1 month ago) |
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is that yellow tint appearing at innoculatio points or all over? it MIGHT just be water deprived mycellium... have any pics?
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MAIA
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Re: Stalled jars [Re: frogsheath]
#572338 - 03/07/02 09:02 AM (23 years, 1 month ago) |
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Try using a PC for sterilizing jars.
As for your jars, untape holes and flip them for a better air exchange.
Peace,
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
 
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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frogsheath
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 Re: Stalled jars [Re: MAIA]
#572462 - 03/07/02 12:05 PM (23 years, 1 month ago) |
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Yes BeppoMarx, the yellow tint occurs at inoculation points and then it develops something around the edges of this that looks like mycelium only splotchier and irregular.  . Thanks for the idea MAIA. I will try untaping and flipping.
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DrAbeLincoln
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Re: Stalled jars [Re: frogsheath]
#573407 - 03/06/02 08:55 PM (23 years, 1 month ago) |
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I'd pitch those and start over...try picking up a pressure cooker at Goodwill...I got an 18 quart all american pressure cooker from goodwill for 30 bucks
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frogsheath
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DrAbe,
yeah, that's what I'm thinkin'. An 18 quart All American from Goodwill for $30 is the ideal sterilizer. But I'll take what I can get at this point. I've had some success with the Martha Stewart stock pot. I can only move up from here. Trip in the Outdoors. Peace.
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LSD_4me
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 Re: Stalled jars [Re: frogsheath]
#574034 - 03/09/02 05:08 AM (23 years, 1 month ago) |
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id be willing to say that yellow is almost definitley contam and not damaged mycellium you can tell becaue the mycellium hasnt even grown there yet :-)
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BeppoMarx
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Re: Stalled jars [Re: frogsheath]
#574110 - 03/09/02 09:05 AM (23 years, 1 month ago) |
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i dunno. ive had something very similar to this before.
did that yellow just appear or was it white then it turned yellow?
i wanna say that it was sterilized ok; but your jars are too dry and theyre drying out after using up all the water. and if youre getting repeated jars like this; your mixture very well be off. what recipe exactly are you using and how do you sterilize and cool the jars?
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frogsheath
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Re: Stalled jars [Re: BeppoMarx]
#574225 - 03/09/02 12:48 PM (23 years, 1 month ago) |
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Yeah Bep, the yellow just appeared there at the inoculation points. It happened in approximately 5-7 days. It slowly faded in and then there was this white splotchy mycelium-like growth that appeared in irregular patches around the fucking yellow shit(excuse me). So my recipe is straight PF. I uh, lets see, uh I grind up some organic brwn rice (1/4 cup), add 50ccs of mineral water, then 1/2 cup vermiculite , blend until cohesive, and steam sterilize in my Martha Stewart stock-pot (which is prolly the problem). But this happened twice in row with Maz spores. I hang my head in sorrow. I'll put up sum more pics of this tragedy.
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frogsheath
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 Re: Stalled jars [Re: frogsheath]
#574240 - 03/09/02 01:18 PM (23 years, 1 month ago) |
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These pictures aren't too good either but can you see the yellowing? It seems like it fits the area where spore solution came out of the needle. This has nothing to, do with the original post which was my stalled jars however. That is another problem. MAIA gave me some help there and it seems to be working. I'll put up some pics of those jars too.   There is the splotchy mycelium-like growth beginning on this one. These were inoculated on the 2nd with Mazatec spores.
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BeppoMarx
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Re: Stalled jars [Re: frogsheath]
#574255 - 03/09/02 01:47 PM (23 years, 1 month ago) |
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so youre saying that this only happened with the maztec strain? was there 1 syringe used for both innocculation dates; or 2 seperate syringes? and you have another strain or batch of jars that are growing ok? if so its probably the syringe not your method; that crock pot should work fine.
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girlskater
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 Re: Stalled jars [Re: frogsheath]
#574258 - 03/09/02 01:48 PM (23 years, 1 month ago) |
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i have the same problems. the recent batch i made were stained... almost flouresant yellow... some grow partcially... now the mycellium is pulling away from the jar. i've been doing what i've been doing all along. in the past i've never had this problem. i recently took about 3 out... broke them up and cased them in a tray. strange thing about the staining is that not all is contamination.
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frogsheath
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Re: Stalled jars [Re: girlskater]
#574295 - 03/09/02 02:34 PM (23 years, 1 month ago) |
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That's weird girlskater. You say some of the mycelia is healthy? I wouldn't call mine fluorescent. It's just an amber tint. This happened to my last tewo batches of 12 and 10 jars respectively. They were from two Mazatec syringes. When I tossed the first batch of 12 I can't say there was much of an odor, and there was no dust-cloud of spores when I slammed 'em out. This was about a week or so after I first noticed the yellow stains. There was some splotchy mycelia-like stuff starting to colonize but I wasn't patient enough to see what would happen. It just looked sketchy. You have any pics?
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frogsheath
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Re: Stalled jars [Re: BeppoMarx]
#574327 - 03/09/02 03:16 PM (23 years, 1 month ago) |
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Thanks Bep, I'm still gonna get a pressure-cooker though 'cause I've got other problems i.e. stalled jars which may also be contaminated. These are inoculated 1-29. However, MAIA gave me the untape and flip tek and LOOK!  after a few days these old jars are O'colonizin' again. I also put them in a warmer room thaen where they were before.
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MAIA
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Re: Stalled jars [Re: frogsheath]
#575553 - 03/11/02 03:52 AM (23 years, 1 month ago) |
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Nice! I hope everything turns out well. Keep'em going and post pics to see how's dread contam evolving, hope it doens't.
Peace,
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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frogsheath
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Re: Stalled jars *DELETED* [Re: MAIA]
#580418 - 03/16/02 07:41 AM (23 years, 1 month ago) |
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Post deleted by frogsheath
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frogsheath
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Re: Stalled jars *DELETED* [Re: frogsheath]
#580510 - 03/16/02 10:55 AM (23 years, 1 month ago) |
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Uh, I guess I messed up this thread by trying to edit my original post. So, here's the "stalled jars"  The red lines show where the mycelium stalled before. The untape and flip tek worked nice. Here's 2 of the Mazatapec jars   Girlskater, you said you had the same problem. How are yours turning out?
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frogsheath
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Re: Stalled jars - NO MORE! (update) [Re: frogsheath]
#584162 - 03/20/02 11:05 AM (23 years, 1 month ago) |
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Hey all!
The Mazatapec 1/2 pints are now all colonizing like
mad (with very healthy looking mycelium). I am
referring to the ones with the weird yellow stain
at the inoculation points. I am baffled. What
was the yellow stain? Anybody else have this?
The other "stalled jars" are quickly finishing now.
I have much condensation - will probably use the
Hippie straw tek - but the mycelium is looking strong.
Thanks MAIA! I really almost threw in the towel for
those jars (4 of them were inoculated 1-29 !).
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MAIA
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Re: Stalled jars - NO MORE! (update) [Re: frogsheath]
#584929 - 03/21/02 03:07 AM (23 years, 1 month ago) |
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Best wishes for that batch!
What do you have in mind for a terrarium ?
Peace,
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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frogsheath
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 Re: Stalled jars - NO MORE! (update) [Re: MAIA]
#584973 - 03/21/02 04:42 AM (23 years, 1 month ago) |
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Good question. I've been thinking about going with
a big Rubbermaid one. I use a 10-gallon aquarium
now and it works fine for about 5 cakes. It is high-
maintenance too. I'll be birthing 9 cakes in the next
couple weeks, then 10 more, then (hopefully) 17
more (inoculated 3-17). I did purchase a pc and
am very pleased with it. I doubt if I'm quite ready
for casing. My biggest problem (in my hut) seems
to be regulating temperature. I live in Chicago. By
the way MAIA those contamination posts (yeast)
you did were interesting.
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MAIA
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Re: Stalled jars - NO MORE! (update) [Re: frogsheath]
#585100 - 03/21/02 09:14 AM (23 years, 1 month ago) |
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Why don't you consider using some kind of heat source, like an aquarium heater inside a jar or something. If you use the search option you'll find an interesting threat by searching with "The problem of heat". If i can remember Anno showed us two overlaped rubbermaid bins with water between them and a aquarium heater, that was for an incubator, but i'm thinking about adapting and transforming that design and make a terrarium out of it, it's very easy, instead of jars, put 2" perlite, 1" h2o2+h20 (1:9 ratio), a transparent cover (the top must allow light to reach the interior) and an air pump(could be potional), cakes inside and lets rock!!!
Peace,
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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frogsheath
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 Re: Stalled jars - NO MORE! (update) [Re: MAIA]
#585570 - 03/21/02 06:10 PM (23 years, 1 month ago) |
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Yeah, I saw a description of that incubator. I believe it
was by Roadkill. That would solve my incubation temp
issue. As far as the fruiting chamber, I don't really have
an issue with temps too much (yet). Right now in Chicago
we have temps in the teens (nice spring weather). My
landlord is always messing with the heat (bless his heart)
and he rarely gets it "right". I adjust the level in here by
cracking windows or turning on the stove, and I can pretty
much maintain between 68 and 78 degrees. That seems
o.k. for fruiting but not incubating. However, after spring
Chicago is notorious for hellish summers. THAT might be
a problem. I'm hoping to finish growing this batch before
the heatwave comes (or perhaps it won't come this year-
you never can tell here) So, your idea is interesting. Perhaps
some modifications for high external temps- maybe venting
a small air-conditioner to the fruiting chamber- are in order.
I doubt if I'm gonna go that far - but never say never.
Anyway, thanks for the input (and the search idea- I'm
fairly new at computers too).
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frogsheath
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 Re: Stalled jars - NO MORE! (update) [Re: MAIA]
#586638 - 03/22/02 06:18 PM (23 years, 1 month ago) |
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HOLY FUCKIN' SHIT!!! Those Maz jars are FLYING through
colonization! The other jars are almost ready to birth! Yeah!!
And I noc'ed up 17 jars of Hawaiian and there takin' off like
gangbusters (a colloqial expression from here) too!! I think
the difference is from when I moved 'em into my bedroom and
put 'em next to the radiator there. Heat IS important! Of
course the UNTAPE and FLIP tek turned around those other jars
from the get-go.
Peace out
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noisycricket
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Re: Stalled jars - NO MORE! (update) [Re: MAIA]
#846205 - 08/27/02 06:32 AM (22 years, 7 months ago) |
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This is great. Ive always wanted to know what to do if jars stall. 
--------------------
I'm just a field researcher for Hitchhikers Guide to The Galaxy.
My entry so far.... "shrooms: Mostly harmless"
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kintos
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Re: Stalled jars - NO MORE! (update) [Re: frogsheath]
#7121720 - 07/02/07 10:05 PM (17 years, 9 months ago) |
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hey im having a similar problem with my jars. the myc is all splotchy and greyish. how did you fix your jars? just wait them out or inject more water?
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laughingbuddha
That's freakinAWESOME!
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Re: Stalled jars - NO MORE! (update) [Re: kintos]
#7121794 - 07/02/07 10:28 PM (17 years, 9 months ago) |
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zenthor, did you notice that this thread is five years old? the last post was in 2002.
anyways, if you have a contam problem you'll want to post it in the contamination forum. a picture really helps.
the mycelium is greyish? mycelium should be fluffy white. if your jar is some other color, it probably got contaminated somewhere along the line. i might wait it out another week or two and see what happens. but remember, if your jar turns black, through the whole thing out or at least dump the black stuff outside, downwind from you. apparently black mold is the kind that will kill you. good luck.
***===LB===***
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"The more I read, the more I meditate; and the more I meditate, the more certain I am that I know nothing." -Voltaire
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