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OfflineTrancedShroom
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How?
    #5717292 - 06/05/06 11:09 PM (17 years, 7 months ago)

Awhile back, I bought a mushroom kit from a headshop. The kit contained a container, a substrate bag filled with a cottonswap covered with spores and rye, and compost.

The grow was quite successful but expensive.

I was thinking how could you just untwist the bag and let the cottonswap drop onto the rye when shaken and spawn mycelium without sterility?

If this is true, that they did not pre-serilize it, could I do the same thing?


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: How? [Re: TrancedShroom]
    #5717318 - 06/05/06 11:15 PM (17 years, 7 months ago)

crazy.

curious about more details.

detail out the materials and procedure a bit.


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OfflineTrancedShroom
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Re: How? [Re: monstermitch]
    #5717363 - 06/05/06 11:25 PM (17 years, 7 months ago)

Fool proof:

Take out bag containing cottonswab and rye. The cottonswab and rye are seperated by a twisty wire. Undo twisty and the cottonswab will fall into the rye. Set in a dark place with temperature 75-80 degrees farenheit until bag is colonized.

Take compost and put into original container that has 1/8inch drilled holes(12 on each long side) and break rye berries into clumps and combine evenly with compost.

Put container in a 75-85 degree setting and do not open until fruiting.


Thats it. I gained a total of 1lb-2lb's of fresh shrooms from this.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: How? [Re: TrancedShroom]
    #5717408 - 06/05/06 11:38 PM (17 years, 7 months ago)

their rye is sterilized
the bag is sterilized
the cottonswab is sterilized and placed into the bag in a sterile manner
the compost is pasteurized (? no experience with compost)

this would be my guess anyway...

fool proof for the buyer maybe...
I highly doubt that you can re-create a similar setup with no sterile practices.


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OfflineTrancedShroom
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Re: How? [Re: monstermitch]
    #5717428 - 06/05/06 11:44 PM (17 years, 7 months ago)

How would you sterilize the bag and the rye?


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: How? [Re: monstermitch]
    #5717431 - 06/05/06 11:45 PM (17 years, 7 months ago)

make a rye bag and pressure cook it.
place bag in front of flow hood and apply twist tie
open top of bag and drop in sterile cottonswab
(this was soaking in a sterile spore solution)
impulse seal bag above the cotton swab.
pasteurize compost and leave in bag/jar/whatever.
add a container for fruiting.

can be done for sure.
interesting idea.

has to have sterility in the process.


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InvisibleAtheist
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Re: How? [Re: monstermitch]
    #5717507 - 06/06/06 12:04 AM (17 years, 7 months ago)

whats cottonswab?


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: How? [Re: Atheist]
    #5717531 - 06/06/06 12:18 AM (17 years, 7 months ago)

a cotton ball soaked in spore solution.

don't like using cotton though...


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OfflineRogerRabbitM
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Re: How? [Re: monstermitch]
    #5718112 - 06/06/06 07:13 AM (17 years, 7 months ago)

Quote:

monstermitch said:
make a rye bag and pressure cook it.
place bag in front of flow hood and apply twist tie
open top of bag and drop in sterile cottonswab
(this was soaking in a sterile spore solution)
impulse seal bag above the cotton swab.
pasteurize compost and leave in bag/jar/whatever.
add a container for fruiting.

can be done for sure.
interesting idea.

has to have sterility in the process.




Perhaps you can teach us how one can grow mushrooms from a sterile spore solution. What good would that do? Spores by their very nature are not sterile. If you sterilze them, no mushrooms for you.

Homestead developed the process he's describing to use in their kits. The bag of rye is sealed with a twist tie just above the grains and PC'd. After it cools, a spore swab is dropped into the bag on TOP of the twist tie, then the bag is impulse sealed on the other side of the spore swab. When ready to use, simply remove the twist tie and the spores drop into the sterile grains.

There's also nothing wrong with cotton. It's not a bad
substrate/casing material actually.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisiblecreamcorn
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Re: How? [Re: RogerRabbit]
    #5718118 - 06/06/06 07:18 AM (17 years, 7 months ago)

man homestead kits have come a ways... i remember working with an old one years back (the original perhaps) that had you innoc agar plates from a print with a loop... transfer isolate to another plate... inoculate test tubes of grain... then spawn to their bag of compost... all the very things a newbie is scared of these days :-)


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OfflineBabo911
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Re: How? [Re: creamcorn]
    #5718128 - 06/06/06 07:28 AM (17 years, 7 months ago)

I remember my first spores were from PF and they came with growing instructions.

I want to know what head shop sold spores with a kit.... and I don't think the ones he got in the kit were eatables


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
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Re: How? [Re: RogerRabbit]
    #5718357 - 06/06/06 09:46 AM (17 years, 7 months ago)

Quote:

RogerRabbit said:
Quote:

monstermitch said:
make a rye bag and pressure cook it.
place bag in front of flow hood and apply twist tie
open top of bag and drop in sterile cottonswab
(this was soaking in a sterile spore solution)
impulse seal bag above the cotton swab.
pasteurize compost and leave in bag/jar/whatever.
add a container for fruiting.

can be done for sure.
interesting idea.

has to have sterility in the process.




Perhaps you can teach us how one can grow mushrooms from a sterile spore solution.  What good would that do?  Spores by their very nature are not sterile. If you sterilze them, no mushrooms for you. 

Homestead developed the process he's describing to use in their kits.  The bag of rye is sealed with a twist tie just above the grains and PC'd.  After it cools, a spore swab is dropped into the bag on TOP of the twist tie, then the bag is impulse sealed on the other side of the spore swab.  When ready to use, simply remove the twist tie and the spores drop into the sterile grains.

There's also nothing wrong with cotton.  It's not a bad
substrate/casing material actually.
RR





okay, my bad. 
the water is sterile and the spores obviously are not.
the cotton must be sterile too.

so everything but the spores is sterile.

sorry, I didn't get that perfect.

p.s.  -  killer picture :thumbup:


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InvisibleAtheist
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Re: How? [Re: monstermitch]
    #5718364 - 06/06/06 09:49 AM (17 years, 7 months ago)

kind of freaky lol


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Registered: 02/10/06
Posts: 3,911
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Re: How? [Re: Atheist]
    #5718368 - 06/06/06 09:50 AM (17 years, 7 months ago)

yeah, nice braclet too.

the man surely has a sense of humor.

gotta love it.


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