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TrancedShroom
Mr. Hanky


Registered: 03/08/06
Posts: 8,002
Loc: Rippin Waves
Last seen: 12 years, 4 months
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How?
#5717292 - 06/05/06 11:09 PM (17 years, 7 months ago) |
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Awhile back, I bought a mushroom kit from a headshop. The kit contained a container, a substrate bag filled with a cottonswap covered with spores and rye, and compost.
The grow was quite successful but expensive.
I was thinking how could you just untwist the bag and let the cottonswap drop onto the rye when shaken and spawn mycelium without sterility?
If this is true, that they did not pre-serilize it, could I do the same thing?
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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crazy.
curious about more details.
detail out the materials and procedure a bit.
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TrancedShroom
Mr. Hanky


Registered: 03/08/06
Posts: 8,002
Loc: Rippin Waves
Last seen: 12 years, 4 months
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Fool proof:
Take out bag containing cottonswab and rye. The cottonswab and rye are seperated by a twisty wire. Undo twisty and the cottonswab will fall into the rye. Set in a dark place with temperature 75-80 degrees farenheit until bag is colonized.
Take compost and put into original container that has 1/8inch drilled holes(12 on each long side) and break rye berries into clumps and combine evenly with compost.
Put container in a 75-85 degree setting and do not open until fruiting.
Thats it. I gained a total of 1lb-2lb's of fresh shrooms from this.
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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their rye is sterilized the bag is sterilized the cottonswab is sterilized and placed into the bag in a sterile manner the compost is pasteurized (? no experience with compost)
this would be my guess anyway...
fool proof for the buyer maybe... I highly doubt that you can re-create a similar setup with no sterile practices.
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TrancedShroom
Mr. Hanky


Registered: 03/08/06
Posts: 8,002
Loc: Rippin Waves
Last seen: 12 years, 4 months
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How would you sterilize the bag and the rye?
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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make a rye bag and pressure cook it. place bag in front of flow hood and apply twist tie open top of bag and drop in sterile cottonswab (this was soaking in a sterile spore solution) impulse seal bag above the cotton swab. pasteurize compost and leave in bag/jar/whatever. add a container for fruiting.
can be done for sure. interesting idea.
has to have sterility in the process.
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Atheist
Stranger


Registered: 01/24/06
Posts: 13,705
Loc: USA
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whats cottonswab?
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: How? [Re: Atheist]
#5717531 - 06/06/06 12:18 AM (17 years, 7 months ago) |
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a cotton ball soaked in spore solution.
don't like using cotton though...
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Quote:
monstermitch said: make a rye bag and pressure cook it. place bag in front of flow hood and apply twist tie open top of bag and drop in sterile cottonswab (this was soaking in a sterile spore solution) impulse seal bag above the cotton swab. pasteurize compost and leave in bag/jar/whatever. add a container for fruiting.
can be done for sure. interesting idea.
has to have sterility in the process.
Perhaps you can teach us how one can grow mushrooms from a sterile spore solution. What good would that do? Spores by their very nature are not sterile. If you sterilze them, no mushrooms for you.
Homestead developed the process he's describing to use in their kits. The bag of rye is sealed with a twist tie just above the grains and PC'd. After it cools, a spore swab is dropped into the bag on TOP of the twist tie, then the bag is impulse sealed on the other side of the spore swab. When ready to use, simply remove the twist tie and the spores drop into the sterile grains.
There's also nothing wrong with cotton. It's not a bad substrate/casing material actually. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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man homestead kits have come a ways... i remember working with an old one years back (the original perhaps) that had you innoc agar plates from a print with a loop... transfer isolate to another plate... inoculate test tubes of grain... then spawn to their bag of compost... all the very things a newbie is scared of these days :-)
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Babo911
Pooper Scooper


Registered: 09/05/05
Posts: 1,745
Loc: Arizona Bay
Last seen: 10 years, 7 months
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I remember my first spores were from PF and they came with growing instructions.
I want to know what head shop sold spores with a kit.... and I don't think the ones he got in the kit were eatables
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Ronhero on the Growery is me
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Quote:
RogerRabbit said:
Quote:
monstermitch said: make a rye bag and pressure cook it. place bag in front of flow hood and apply twist tie open top of bag and drop in sterile cottonswab (this was soaking in a sterile spore solution) impulse seal bag above the cotton swab. pasteurize compost and leave in bag/jar/whatever. add a container for fruiting.
can be done for sure. interesting idea.
has to have sterility in the process.
Perhaps you can teach us how one can grow mushrooms from a sterile spore solution. What good would that do? Spores by their very nature are not sterile. If you sterilze them, no mushrooms for you.
Homestead developed the process he's describing to use in their kits. The bag of rye is sealed with a twist tie just above the grains and PC'd. After it cools, a spore swab is dropped into the bag on TOP of the twist tie, then the bag is impulse sealed on the other side of the spore swab. When ready to use, simply remove the twist tie and the spores drop into the sterile grains.
There's also nothing wrong with cotton. It's not a bad substrate/casing material actually. RR
okay, my bad. the water is sterile and the spores obviously are not. the cotton must be sterile too.
so everything but the spores is sterile.
sorry, I didn't get that perfect.
p.s. - killer picture
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Atheist
Stranger


Registered: 01/24/06
Posts: 13,705
Loc: USA
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kind of freaky lol
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monstermitch
Growing in Bags Doesn't Work


Registered: 02/10/06
Posts: 3,911
Loc: Arizona Bay
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Re: How? [Re: Atheist]
#5718368 - 06/06/06 09:50 AM (17 years, 7 months ago) |
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yeah, nice braclet too.
the man surely has a sense of humor.
gotta love it.
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