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Offlineno_satori
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Registered: 05/22/06
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Last seen: 17 years, 4 months
Clarification (LC)
    #5716050 - 06/05/06 06:08 PM (17 years, 7 months ago)

First time working with cultures, and I have a quick question.

Spawining substrate from a spent cake or casing is ineffective.
What about making an LC from a spent cake or casing? Seems like it would have the same issues...


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: Clarification (LC) [Re: no_satori]
    #5716065 - 06/05/06 06:13 PM (17 years, 7 months ago)

I wouldn't drop a dirty piece of substrate into my LC.

Just start over with spores.
You did make prints, right?
Next time look into cloning as well.

put your substrate outside with a layering technique.


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OfflineBPJohnny
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Re: Clarification (LC) [Re: monstermitch]
    #5716083 - 06/05/06 06:18 PM (17 years, 7 months ago)

yes it will have the same issues. however you could maybe drop a pin
into an LC? in a sterile matter of course


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Offlineno_satori
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Re: Clarification (LC) [Re: monstermitch]
    #5716084 - 06/05/06 06:18 PM (17 years, 7 months ago)

Thx


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: Clarification (LC) [Re: BPJohnny]
    #5716086 - 06/05/06 06:19 PM (17 years, 7 months ago)

Quote:

BPJohnny said:
yes it will have the same issues. however you could maybe drop a pin
into an LC? in a sterile matter of course




worth a shot.
I think I'll try soon just because.


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OfflineBPJohnny
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Re: Clarification (LC) [Re: monstermitch]
    #5716091 - 06/05/06 06:21 PM (17 years, 7 months ago)

RR got me thinking about that, wanna try it with LC and skip the agar


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Invisiblemusher_420
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Re: Clarification (LC) [Re: BPJohnny]
    #5716113 - 06/05/06 06:28 PM (17 years, 7 months ago)

Instead of using a pin I'd probably use the inside tissue of a larger mushroom's stock (stipe) that hasn't been exposed to outside contams. Just as an extra precaution. Rip apart the stock instead of cutting it. This is so that contams from the outside of the stock aren't drawn to your clone tissue on the knife blade. I suppose an H2O2 dunk would be helpful if using a pin.

I once took a chunk of stock and put it into a sterile half pint jar half full of sterile water that fit the blade assembly of my blender. Gave it a quick blend, then inside my glove box sucked up the blueish solution with a syringe and injected it into a pf cake. After about 5 days I could see growth eventually the jars colonized fully contam free and fruited the same as the original cakes.

There's a pictoral of the procedure here on the shroomery but I haven't been able to find it.


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Edited by musher_420 (06/05/06 06:34 PM)


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OfflineBPJohnny
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Re: Clarification (LC) [Re: musher_420]
    #5716181 - 06/05/06 06:53 PM (17 years, 7 months ago)



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