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Offlineorissacube
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To H2O2 or not H2O2
    #5713840 - 06/05/06 02:15 AM (17 years, 7 months ago)

OK I've done my search on this subject and can't seen to find a definitive answer.

ie is it safe to use H2O2 in my misting solution.

Some posts say this will burn the top of the mycelium and only use it in the incubation chamber where it doesn't come into contact with the mycelium.

Yet some people do use it to mist.

Whats the answer safe or not?


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Offlinehyphae
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Re: To H2O2 or not H2O2 [Re: orissacube]
    #5714021 - 06/05/06 05:56 AM (17 years, 7 months ago)

H2O2 retards growth slightly but myc recovers well depending on it's concentration. I personally only use "clean" water and h2o2 if ever in doubt (rarely). You can certainly use it but keep it weak.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineBabo911
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Re: To H2O2 or not H2O2 [Re: hyphae]
    #5714028 - 06/05/06 06:06 AM (17 years, 7 months ago)

Quote:

hyphae said:
I personally only use "clean" water and h2o2 if ever in doubt




I assume you mean distilled water?


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Offlinehyphae
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Re: To H2O2 or not H2O2 [Re: Babo911]
    #5714040 - 06/05/06 06:20 AM (17 years, 7 months ago)

Quote:

Babo911 said:
Quote:

hyphae said:
I personally only use "clean" water and h2o2 if ever in doubt




I assume you mean distilled water?



Distilled, sterilized or boiled.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleUnderNose
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Re: To H2O2 or not H2O2 [Re: hyphae]
    #5714046 - 06/05/06 06:26 AM (17 years, 7 months ago)

what about De-mineralized water for misting and LC's

Is says not suitable for drinking

Suggested uses
Laboratory solutions, Batteries, Car Radiators, Steam irons


--------------------
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Offlinetallgreen
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Re: To H2O2 or not H2O2 [Re: UnderNose]
    #5715209 - 06/05/06 02:16 PM (17 years, 7 months ago)

I water my plants with H2O2. It's only dangerous because of the oxidation that comes from the extra oxygen atom on the molecule, aside from that it's just water, which is what it becomes when it breaks down. So it's just oxygen we're talking about, not some kind of rare chemical burn. Just make sure it's extremely diluted and it should be fine. When I water my plants I use the 3% and maybe a ratio of 1:20 (H2O2:Water). That could be too concentrated since I have never used it on shrooms before, but the plants love it. My 2 cents.


--------------------
Nothing you can know that isn't known.
Nothing you can see that isn't shown.
Nowhere you can be that isn't where you're meant to be.
It's easy.
All you need is love.
- The Beatles


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OfflineCoolMojo
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Re: To H2O2 or not H2O2 [Re: tallgreen]
    #5715623 - 06/05/06 03:50 PM (17 years, 7 months ago)

Tallgreen: the extra oxygen molecule is weak and it breaks off, when it breaks off its kinda a mini explosion and it kills living tissue. A 3% solution diluted like u have will kill very little tissue and the mycelium easily recovers.

As for a rare chemical burn, well a 50% solution or better poured onto wood will instantly go up in flames. So yes it is kinda a rare chemical burn :o) but in the concentrations we use, nah nothing to worry about.


Edited by CoolMojo (06/05/06 03:51 PM)


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Offlinehyphae
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Re: To H2O2 or not H2O2 [Re: CoolMojo]
    #5716295 - 06/05/06 07:27 PM (17 years, 7 months ago)

Quote:

CoolMojo said:
Tallgreen: the extra oxygen molecule is weak and it breaks off, when it breaks off its kinda a mini explosion and it kills living tissue. A 3% solution diluted like u have will kill very little tissue and the mycelium easily recovers.

As for a rare chemical burn, well a 50% solution or better poured onto wood will instantly go up in flames. So yes it is kinda a rare chemical burn :o) but in the concentrations we use, nah nothing to worry about.


Glad to hear you know what your talking about! :thumbup:
Tallgreen rinse your mouth with 3% peroxide all the while remembering how fragile mycelium actually is. Active 3% will knock down myc far more than plain old clean water.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineRogerRabbitM
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Re: To H2O2 or not H2O2 [Re: hyphae]
    #5716485 - 06/05/06 08:15 PM (17 years, 7 months ago)

Here's a question for the science geeks. When pesticides are used routinely, the insects soon grow immune to them. I'm wondering if there's any chance that using peroxide as a matter of routine as opposed to as needed is going to select for resistance in bacteria and molds. After all, only the trich spores or cobweb that are tolerant of the peroxide are going to germinate, and then reproduce. This is the reason I personally only use peroxide to kill an active cobweb infestion, and of course it's good to dip cloned material in peroxide before placing on agar.

Also, note that using peroxide to kill bacteria on a casing is counterproductive.
RR


--------------------
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OfflineCoolMojo
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Re: To H2O2 or not H2O2 [Re: RogerRabbit]
    #5716510 - 06/05/06 08:22 PM (17 years, 7 months ago)

Roger: Since peroxide isn't actually poisoning anything probly not. Its not a matter of biology but of physics, but an organism could in theory develope a way around it. It woulnd't be a matter of immunity but more of a defense against the energy released when the bond between the weakly bonded oxygen molecule is released.

Essentially the asnwer is possibly, but not likly and not in the way your thinking. It would be more like us developing the ability to be bullet proof...yes it IS possible but...


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Offlinetallgreen
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Re: To H2O2 or not H2O2 [Re: hyphae]
    #5716634 - 06/05/06 08:47 PM (17 years, 7 months ago)

Quote:

hyphae said:
Quote:

CoolMojo said:
Tallgreen: the extra oxygen molecule is weak and it breaks off, when it breaks off its kinda a mini explosion and it kills living tissue. A 3% solution diluted like u have will kill very little tissue and the mycelium easily recovers.

As for a rare chemical burn, well a 50% solution or better poured onto wood will instantly go up in flames. So yes it is kinda a rare chemical burn :o) but in the concentrations we use, nah nothing to worry about.


Glad to hear you know what your talking about! :thumbup:
Tallgreen rinse your mouth with 3% peroxide all the while remembering how fragile mycelium actually is. Active 3% will knock down myc far more than plain old clean water.




Are you talking to me or CoolMojo?  I have rinsed my mouth with 3%, yeah, it's pretty volatile.  Ahh, nice clean, "just brushed" feeling.  I know mycelium is fragile, it would be crazy to spray 3% directly on, all I'm saying is I use it a lot in various things and when diluted, like I said 1:20 or more, it's fairly gentle.  One time My dog ate a pound of chocolate and I had to dump a bottle down her throat to induce vomiting.  :eek:

CoolMojo: yeah you're right I guess that is kinda rare.  I just meant it's not some unknown molecule that does who-knows-what patented by scjohnson or something.


--------------------
Nothing you can know that isn't known.
Nothing you can see that isn't shown.
Nowhere you can be that isn't where you're meant to be.
It's easy.
All you need is love.
- The Beatles


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Offlinehyphae
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Re: To H2O2 or not H2O2 [Re: tallgreen]
    #5716672 - 06/05/06 08:55 PM (17 years, 7 months ago)

Quote:

tallgreen said:
Quote:

hyphae said:
Quote:

CoolMojo said:
Tallgreen: the extra oxygen molecule is weak and it breaks off, when it breaks off its kinda a mini explosion and it kills living tissue. A 3% solution diluted like u have will kill very little tissue and the mycelium easily recovers.

As for a rare chemical burn, well a 50% solution or better poured onto wood will instantly go up in flames. So yes it is kinda a rare chemical burn :o) but in the concentrations we use, nah nothing to worry about.


Glad to hear you know what your talking about! :thumbup:
Tallgreen rinse your mouth with 3% peroxide all the while remembering how fragile mycelium actually is. Active 3% will knock down myc far more than plain old clean water.




Are you talking to me or CoolMojo?  I have rinsed my mouth with 3%, yeah, it's pretty volatile.  Ahh, nice clean, "just brushed" feeling.  I know mycelium is fragile, it would be crazy to spray 3% directly on, all I'm saying is I use it a lot in various things and when diluted, like I said 1:20 or more, it's fairly gentle.  One time My dog ate a pound of chocolate and I had to dump a bottle down her throat to induce vomiting.  :eek:

CoolMojo: yeah you're right I guess that is kinda rare.  I just meant it's not some unknown molecule that does who-knows-what patented by scjohnson or something.




I was referring to CoolMojo BTW. I also used diluted h2o2 in all my coolmists for quite some time molds still became present after time though. I then upgraded to the Homer2 with iodine.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineCoolMojo
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Re: To H2O2 or not H2O2 [Re: tallgreen]
    #5716686 - 06/05/06 09:01 PM (17 years, 7 months ago)

Yeah I peroxide the water in a higher conecentration when I dunk and I never have a problem. I didn't mean you shouldn't use it, I was just shedding some light on what is actually involved.


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Offlinetallgreen
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Re: To H2O2 or not H2O2 [Re: CoolMojo]
    #5716705 - 06/05/06 09:06 PM (17 years, 7 months ago)

I had wondered about iodine. We used to use it on backpacking trips when we ran out of clean water. I'm assuming you just put that in water that is evaporated, not sprayed, correct?


--------------------
Nothing you can know that isn't known.
Nothing you can see that isn't shown.
Nowhere you can be that isn't where you're meant to be.
It's easy.
All you need is love.
- The Beatles


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Offlinehyphae
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Re: To H2O2 or not H2O2 [Re: tallgreen]
    #5716716 - 06/05/06 09:09 PM (17 years, 7 months ago)

Correct


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineOatman2000
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Re: To H2O2 or not H2O2 [Re: hyphae]
    #5722301 - 06/07/06 09:49 AM (17 years, 7 months ago)

iodine huh? Interested....

Please tell me more.


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