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kilroy
Hightimes



Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit
Last seen: 13 years, 3 months
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Something for newbies
#5703222 - 06/02/06 08:16 AM (17 years, 7 months ago) |
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I myself am a newbie, but have done alot of reading and pming and chatting with the more experienced people of this wonderful type of study. I have one suggestion for all of you just starting out. Make up only 3 jars at a time to start. Reason #1 It will save on the syringe as you work out your tek. If you mess up with your on your ingredient then you still have plenty to try another batch. Reason #2 You can do other teks like LC with some of the remainder. I strongly suggest that you try LC anyways for it cuts down on incubating time and you can make 1cc into 20cc easy. I found that the honey LC is the easiest to try first. 1 teaspoon of honey to every 100ml of water PC for 15min at 15psi. If no pc then there is a tek by a member named Otto that shows how to do it by boiling.
I hope this helps some of the new people who are just starting this wonderful study, and the last big advice I can give you is patients, lots and lots of patients. You are among IMO the best teachers of this type of study, now just remember to try and be good students and listen, read, and research your study. I wish you all GL in your studies
-------------------- IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.
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SDP
ChronicAficionado



Registered: 01/21/05
Posts: 1,297
Last seen: 5 months, 28 days
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Re: Something for newbies [Re: kilroy]
#5703442 - 06/02/06 10:05 AM (17 years, 7 months ago) |
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He does not lie... all hail the way of the liquid culture! 
-SDP
-------------------- Teonanacatl, open up my eyes This sacrament, this prayer, beyond the world of lies Guide me clearly through that which I dont understand Give me strength to find the path Help me fight any demons as you flow through me wholely This is my prayer, that you protect me from evil, and bring me closer to peace And open up my eyes, so i can see things as you do Amen
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Something for newbies [Re: SDP]
#5703533 - 06/02/06 10:32 AM (17 years, 7 months ago) |
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I wouldn't totally agree.
I like to use an entire syringe in one "session", its just a me thing maybe, but I don't feel comfortable hanging on to partially used syringes. You get substrate on/in your needle (especially if doing PF tek where you're jamming the needle right into your mix) and that leaves a prime situation for contams to grow on the needle itself, and upward into the syringe. Maybe it doesn't really happen that way, like I say its a me thing... but once a syringe is used i finish it off all at once. at first when you start spores seem like gold, you went through effort to get them and you don't want to mess them up, but even if you manage to grow a couple mushrooms from it you can make a hundred more where that came from in a few weeks, so don't stress it so much.
I say for your first time make a dozen jars like so:
8 standard substrate jars (1 cc each) 3 liquid culture jars (little less than 1cc each, you only need a few drops) 1 control jar (prepare with the others but no spores at all! treat it like all the others though, even "pretend" its been inoculated, and incubate it along side the other ones. label it... and in fact get into the habit of labelling everything you do, dates, and i like to use a little "id number" that matches up to a journal i keep, with exact recipes used... comes in handy later when you start mixing things up and playing with recipes, keeping track of which cakes/cases are on which flush, etc.)
this covers all of your bases.
with 8 half pinters you got plenty if all goes right, and even if you have to chuck a few you still got some to work with. if you're still paranoid about screwing them all up, prepare them in two separate batches of 4. you'd be a little peeved if you did everything perfect and the end result was 3 measly half pints. 
3 LC's because odds are good at least one will work. if they all work you've got enough LC to shoot another 50-100 jars and won't need to even think about spores again for a long time. karo is even easier for a newbie than honey. honey is more prone to carmelization at PC temps (PC'ing isn't necessary for LC IMO, as you mentioned. i steam sterilize for 30 minutes and save myself the trouble of digging out and setting up the PC.) karo doesn't leave behind any sediments either like honey does, which can be confusing to a first timer who's paranoid about contams.
1 control jar to serve as your indicator. this is super useful in troubleshooting at the beginning. your control jar should do nothing! if it contams, you know you didn't sterilize right. if it stays clean, yet your others contam, you know either your syringe was at fault (unlikely if a vendor syringe, but possible) or your inoculation method was at fault (more likely)
Edited by creamcorn (06/02/06 10:37 AM)
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Something for newbies [Re: kilroy]
#5703647 - 06/02/06 11:01 AM (17 years, 7 months ago) |
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You guys are making my eyes hurt! 
Once you get an LC or two going, experiment all you want! No need to say "Well, I'll use 2 jars for this, 1 jar for that, 1cc for this...."
--------------------
Quick WBS Prep
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creamcorn
mad scientist


Registered: 03/13/06
Posts: 2,962
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Re: Something for newbies [Re: FooMan]
#5703673 - 06/02/06 11:10 AM (17 years, 7 months ago) |
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haha... exactly what i meant, when starting off people tend to be miserly with their spores because they're afraid things won't work. a single successful LC is all you need. 
i do think my idea is a good and efficient plan of attack though for somebody just starting. but then again i'm biased and think all my ideas are good ones.
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Jim


Registered: 04/07/04
Posts: 20,922
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Re: Something for newbies [Re: creamcorn]
#5703676 - 06/02/06 11:11 AM (17 years, 7 months ago) |
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I can stretch a 12cc syringe through 2 dozen pints...
grain transfers are much easier imo once you get a few jars done...
-------------------- Use the Fucking Reply To Feature You Lazy Pieces of Shit! afoaf said: Jim, if you were in my city, I would let you fuck my wife.
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Something for newbies [Re: Jim]
#5703705 - 06/02/06 11:19 AM (17 years, 7 months ago) |
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Quote:
Jim said: grain transfers are much easier imo once you get a few jars done...
Yeah, but G2G transfers require either a glove box or a seriously clean work area. I can do Lc's easily in just about any environment with a little lysol and rubbing alcohol.
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Quick WBS Prep
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Jim


Registered: 04/07/04
Posts: 20,922
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Re: Something for newbies [Re: FooMan]
#5703710 - 06/02/06 11:20 AM (17 years, 7 months ago) |
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maybe i should be ashamed to say I dont fuck with the liquid cultures....
-------------------- Use the Fucking Reply To Feature You Lazy Pieces of Shit! afoaf said: Jim, if you were in my city, I would let you fuck my wife.
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