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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
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Last seen: 16 years, 1 day
Liquid Culture - Carimilized but devloped - Help pls
    #5697897 - 06/01/06 01:18 AM (17 years, 7 months ago)

Hi, So here's my predicament. I added 15g's of dextrose to a 500ml jar (dont ask why so much dextrose)

I pc'd with two jars of rye at 10psi for a hour and a half, well, it carimilized abit (the water turned abit yellowish) but I decided to innoc with B+ anyways.

I seen signs of myc within the first 48 hours of innoculating and I continue to shake the jars on a daily basis but from what I can see there hasn't been anymore significant growth. I can count about 30-50 small peices of myc, if the LC is demonstrating weak growth like this one will it be ok to innoc a few jars with?

It was being incubated at 28c but tommorow im just going to throw it in the fridge and innoc up a tester jar...good idea?


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It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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InvisibleRahz
Alive Again
Male

Registered: 11/10/05
Posts: 9,230
Re: Liquid Culture - Carimilized but devloped - Help pls [Re: Pr0_X]
    #5697964 - 06/01/06 01:32 AM (17 years, 7 months ago)

Hi Pro. Give it 10-14 days before you give up on it. If conditions aren't perfect, growth can be slow. You could test, but I'd leave it out of the fridge until deciding on what to do with it.

I PC LCs 20 minutes, much longer and caramelizing a good bet. 2 birds I know, but 1.5 hours is too much.

Rahz


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rahz

comfort pleasure power love truth awareness peace


"You’re not looking close enough if you can only see yourself in people who look like you." —Ayishat Akanbi


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Offlinekilroy
Hightimes
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Folding@home Statistics
Registered: 04/26/06
Posts: 768
Loc: Deep within my spirit Flag
Last seen: 13 years, 3 months
Re: Liquid Culture - Carimilized but devloped - Help pls [Re: Rahz]
    #5698752 - 06/01/06 08:29 AM (17 years, 7 months ago)

a much easier LC is 1 teaspoon of honey to every 100ml of water, pc for 15 min at 15psi. I have wonderfull results with this one. Rahz is also right 10-14 days is not unheard of for an LC. Also from what I have read to much stuff in an LC can slow down production as well, meaning to thick of an LC. GL


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IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





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