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Offlinesplifner180
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Registered: 03/24/06
Posts: 1,288
Loc: USA, East Coast
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Possible Sterilization of Syringes
    #5688363 - 05/29/06 08:39 PM (17 years, 8 months ago)

Is it possible that my syringes are being inadvertently sterilized by the USPO or something? I'm three days into innoc and no growth. This isn't particularly surprising given the WBS jars but my LCs are just as clear as the day they went in.

I'm really beginning to wonder. This is after two (suspected) duds from a vendor and these are the replacements. And my vendor has a good rep around here.

No growth, even bad growth. Temps have been 83-85F without fail for the entire incubation period. WBS and LC jars were allowed to cool for 2.5 days and 1.5 days, respectively.

I'm completely baffled.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


Edited by splifner180 (05/29/06 08:47 PM)


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InvisibleJoeMama
Registered: 04/28/06
Posts: 154
Re: Possible Sterilization of Syringes [Re: splifner180]
    #5688395 - 05/29/06 08:48 PM (17 years, 8 months ago)

Patience... That is the only thing I can suggest man. Its taken me close to 7 days to see growth in my 2 jars I innoced up. They are growing now :smile: Woot. (These are my first 2 jars BTW)
-Me


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Offlinesplifner180
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Re: Possible Sterilization of Syringes [Re: splifner180]
    #5688403 - 05/29/06 08:49 PM (17 years, 8 months ago)

JoeMama, if my syringes are filled with non-viable spores, what good will patience do me?

The previous setup has been incubating for over a month with no myc. So patience doesn't quite seem to be cutting it.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


Edited by splifner180 (05/29/06 08:51 PM)


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InvisibleAtheist
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Re: Possible Sterilization of Syringes [Re: splifner180]
    #5688467 - 05/29/06 09:04 PM (17 years, 8 months ago)

Wait splif, did the syringes work in an LC or did you go straight to the jar?


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Offlinehyphae
born to grow
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Re: Possible Sterilization of Syringes [Re: Atheist]
    #5688485 - 05/29/06 09:10 PM (17 years, 8 months ago)

Three days and looking for myc? I agree patience is in order at least this time around.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineKaptKid
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Re: Possible Sterilization of Syringes [Re: splifner180]
    #5688490 - 05/29/06 09:11 PM (17 years, 8 months ago)

Were did you get your syringes from?


:sun:


--------------------
Child of the 60's, Tripping ever since.


:sun:


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Offlinesplifner180
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Re: Possible Sterilization of Syringes [Re: KaptKid]
    #5690033 - 05/30/06 09:10 AM (17 years, 7 months ago)

SpicyTunaRoll writes:
"Wait splif, did the syringes work in an LC or did you go straight to the jar? "

I have nine WBS jars and three LC jars. None have shown any growth whatsoever.

hyphae writes:
"Three days and looking for myc? I agree patience is in order at least this time around. "

I was under the impression that LC starts within three days. I would have more patience 'cept a month of patience turned up nothing on my first grow. And this grow is following the same trajectory as the last.

KaptKid writes:
"Were did you get your syringes from?"

Spores101. I purchased one syringe but they sent two. Both came up with nothing for whatever reason. So they replaced that syringe and I also won a syringe, so two came in the mail.

I knocked up my dozen jars on the 26th. The syringes, while in my custody, never exceeded 86F. And I shook the PISS out of those babies. Tapped them against a book for a full minute, too.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Offlinekilroy
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Re: Possible Sterilization of Syringes [Re: splifner180]
    #5690051 - 05/30/06 09:22 AM (17 years, 7 months ago)

I have noticed with my LC that if let it form alittle before you use magnetic stirrer it seems to speed it up alittle. I did one and put it on the magnetic stirrer right away and it took almost 6 days before I saw anything, then on my last one I waited 3 days to start seeing anything then put on the magnetic stirrer at a very low speed just a nough to see a nipple and it started producing alot faster. I feel for all the trouble you have been having with them. I remember my first posting was about sponsers and you had just gotten your first replacement from them. I know this isnt any help but maybe it is time to switch sponsers or matbe try trading something for some prints in the market place, it is what I have done. I will help in any way I can.


--------------------
IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





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Offlinezeegos
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Re: Possible Sterilization of Syringes [Re: kilroy]
    #5690056 - 05/30/06 09:26 AM (17 years, 7 months ago)

I knocked up some LC a couple of days ago and dont have a magnetic stirrer. Hell i cant even swirl it because its a square jar.
How long should first signs of mycelium growth take?
Hoping to get some hpoo jars on the go


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Offlinesplifner180
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Re: Possible Sterilization of Syringes [Re: kilroy]
    #5692073 - 05/30/06 07:18 PM (17 years, 7 months ago)

kilroy writes:
"I have noticed with my LC that if let it form alittle before you use magnetic stirrer it seems to speed it up alittle. I did one and put it on the magnetic stirrer right away and it took almost 6 days before I saw anything, then on my last one I waited 3 days to start seeing anything then put on the magnetic stirrer at a very low speed just a nough to see a nipple and it started producing alot faster."

This batch I stirred all three LCs on the magnetic stirrer for 5 minutes or so, just to get them worked in. They've been left off the stirrer since.

Just checked 'em again. Day 4. Nothing. Grr.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Offlinehyphae
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Re: Possible Sterilization of Syringes [Re: splifner180]
    #5692505 - 05/30/06 08:55 PM (17 years, 7 months ago)

Well I have personally "popped" stubborn spores a few times by raising the temps above 100F. give her a try if you like (overnight).


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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OfflineSnaggletooth
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Re: Possible Sterilization of Syringes [Re: hyphae]
    #5692536 - 05/30/06 09:01 PM (17 years, 7 months ago)

Have I missed this, what are your LCs made from.


--------------------


Atheist Chat


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Offlinesplifner180
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Re: Possible Sterilization of Syringes [Re: Snaggletooth]
    #5696673 - 05/31/06 09:03 PM (17 years, 7 months ago)

Snaggletooth writes:
"Have I missed this, what are your LCs made from."

A tablespoon of honey in 1 pt of water, PC'd for 15 minutes at 15 PSI. Removed, liquid is filtered through two cofee filters, then re-PC'd for another 15 minutes.

The lid itself is agar's tubing+polyfill+RTV style. Or at least I think it's agar's.

I STAND CORRECTED. Every one of my jars popped in the last day. Every one.

So 5 days total. Apparently patience is precisely what I needed.

splif


--------------------
First Grow: Ecuador -> LC -> HPoo/Straw -> Monotub
Build a Do-It-Yourself Magnetic Stirrer in thirty minutes with no money.


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Offlinekilroy
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Re: Possible Sterilization of Syringes [Re: splifner180]
    #5696719 - 05/31/06 09:12 PM (17 years, 7 months ago)

Good to go then, thats great splif, I was hoping this would start going for you.


--------------------
IS NOT THE JOURNEY OF THE TRIP JUST AS IMPORTANT AS REACHING THE DESTINATION.





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