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Offlinesa_mull
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To shake or not to shake (pic)
    #5685705 - 05/29/06 06:52 AM (17 years, 8 months ago)

I forgot to Shake the jar at the inital inoculation. its been about 4 days. should i shake now or wait till it's 30-40% growth?


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Offlinesa_mull
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5685709 - 05/29/06 06:54 AM (17 years, 8 months ago)

sorry he's the pic!!



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OfflineRogerRabbitM
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5685711 - 05/29/06 06:55 AM (17 years, 8 months ago)

I'd shake now, and you probably won't need to again. You also need to remove the foil.
RR


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Offlinesa_mull
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Re: To shake or not to shake (pic) [Re: RogerRabbit]
    #5685744 - 05/29/06 07:33 AM (17 years, 8 months ago)

why remove the foil? won't thatopen it up for contams? the lid is lossened and there is a big hole cut in the top,prolly 1/2 inch. i did this following the "From syringe to print using rye"
but asi look at it now they dochange lids there.. should i have any holes in the lid.

thanx RR


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OfflineBabo911
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5685829 - 05/29/06 08:43 AM (17 years, 8 months ago)

what kind of lid are you useing ?


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Offlinehyphae
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5685849 - 05/29/06 08:48 AM (17 years, 8 months ago)

If you cracked the lids loose then your fine. I actually never used any filters for years during incubating I only loosened the lids a bit never had one single contam! People never like to keep things simple they always think theres a better way, it a shame really. The only thing is remembering to tighten the lids before shaking then reloosening. GL and Welcome


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Getting the most out of your casings!, A pinning strategy.
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Not what is overlay but rather what overlay is
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Offlinesa_mull
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Re: To shake or not to shake (pic) [Re: hyphae]
    #5685905 - 05/29/06 09:16 AM (17 years, 8 months ago)

thanx for theinfo.. i'm just using aregular mason jar lid. with and inoculation hole and a bigger hole punched into it.. it seems to be doing fine. i just shook it about 30 mins ago. i'll keep u posted


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Offlinehyphae
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5685930 - 05/29/06 09:32 AM (17 years, 8 months ago)

Are you using lids with holes and thats it besides the foil?  :whoa:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinesa_mull
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Re: To shake or not to shake (pic) [Re: hyphae]
    #5685932 - 05/29/06 09:34 AM (17 years, 8 months ago)

yes, there isnothing between them, and only now do i see my error. what should i do.i'veonly shook them once and that was about 45 mins ago


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Offlinesa_mull
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5685980 - 05/29/06 10:11 AM (17 years, 8 months ago)

ok i just switched the lids to normal mason jar lids. no hole, lossened


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Offlinehyphae
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5685987 - 05/29/06 10:14 AM (17 years, 8 months ago)

Well theres no guarantee they won't contam now so say a prayer to our shroom God. Best of luck bro!


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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InvisibleOptx
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Re: To shake or not to shake (pic) [Re: hyphae]
    #5686243 - 05/29/06 12:23 PM (17 years, 8 months ago)

tyvek is your friend. foil is dumb.


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Offlinedudefromaz
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Re: To shake or not to shake (pic) [Re: Optx]
    #5686412 - 05/29/06 01:07 PM (17 years, 8 months ago)

lol dude, just go to teh post office and pick up a priority envelope and cut a big circle and screw the jar lid onto it and then walla....filter fo dem bastard mofo contams


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Offlinenugjug
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Re: To shake or not to shake (pic) [Re: dudefromaz]
    #5686459 - 05/29/06 01:17 PM (17 years, 8 months ago)

Just make sure you hold your hand over the tyvek before you shake if you're going to do it that way. When you cut an exact circle expecially when they first come out of the pressure cooker it's really easy to shake it and have the cover come off.


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OfflineNeurogenesis
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Re: To shake or not to shake (pic) [Re: nugjug]
    #5686469 - 05/29/06 01:19 PM (17 years, 8 months ago)

Wouldn't there be an issue with moisture loss using just tyvek?


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Offlinehyphae
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Re: To shake or not to shake (pic) [Re: Neurogenesis]
    #5687213 - 05/29/06 04:26 PM (17 years, 8 months ago)

Quote:

Neurogenesis said:
Wouldn't there be an issue with moisture loss using just tyvek?



Yes there can be IME.


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinehyphae
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Re: To shake or not to shake (pic) [Re: Optx]
    #5687220 - 05/29/06 04:27 PM (17 years, 8 months ago)

Quote:

Optx said:
tyvek is your friend. foil is dumb.



Ah no it's not especially when using lids that have lots of gas exchange, the foil helps the culture hold it's moisture content nicely. WORD!


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinesa_mull
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Re: To shake or not to shake (pic) [Re: hyphae]
    #5692755 - 05/30/06 09:48 PM (17 years, 7 months ago)

Alright i need some helpnow... can u tell me if these jar need to be thrown out? these are theones with the crappy lids that i had to change. they have grown crazy in the last day and a half. but doesn't look like mycelium. maybe cobweb mold. please help.








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Offlinenuk1m
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5692839 - 05/30/06 10:09 PM (17 years, 7 months ago)

that IS mycelium, doesn't look contamed to me your good to go :laugh:


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Invisibledog
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Re: To shake or not to shake (pic) [Re: sa_mull]
    #5693701 - 05/31/06 02:24 AM (17 years, 7 months ago)

Quote:

that IS mycelium, doesn't look contamed to me your good to go




:thumbup:


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Offlinesa_mull
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Re: To shake or not to shake (pic) [Re: dog]
    #5693980 - 05/31/06 06:45 AM (17 years, 7 months ago)

So now do I wait till It's fully colonized, then shake again. or that's it. im thinking i'm going to try to do a casing with one. and theother 2 i'm gonna colonize a few more jars.

thanx for the help guys :smile:

sa_mull


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Shop: Mushroom-Hut Liquid Cultures, Substrate Mix   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


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